Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DSM2777_RS23300 Genome accession   NZ_CP014608
Coordinates   4969451..4970467 (+) Length   338 a.a.
NCBI ID   WP_061555257.1    Uniprot ID   A0AA91EJ37
Organism   Obesumbacterium proteus strain DSM 2777     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4964451..4975467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DSM2777_RS23265 (DSM2777_23265) - 4964805..4965434 (+) 630 WP_211271125.1 fimbria/pilus periplasmic chaperone -
  DSM2777_RS23270 (DSM2777_23270) - 4965437..4965949 (+) 513 WP_061555253.1 CS1 type fimbrial major subunit -
  DSM2777_RS23275 (DSM2777_23275) hemW 4966009..4967139 (-) 1131 WP_061555254.1 radical SAM family heme chaperone HemW -
  DSM2777_RS23280 (DSM2777_23280) rdgB 4967132..4967725 (-) 594 WP_061555255.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  DSM2777_RS23285 (DSM2777_23285) yggU 4967779..4968081 (-) 303 WP_046458209.1 DUF167 family protein YggU -
  DSM2777_RS23290 (DSM2777_23290) - 4968087..4968641 (-) 555 WP_025798710.1 YggT family protein -
  DSM2777_RS23295 (DSM2777_23295) - 4968715..4969428 (-) 714 WP_061555256.1 YggS family pyridoxal phosphate-dependent enzyme -
  DSM2777_RS23300 (DSM2777_23300) pilT 4969451..4970467 (+) 1017 WP_061555257.1 type IV pilus twitching motility protein PilT Machinery gene
  DSM2777_RS23305 (DSM2777_23305) - 4970532..4970783 (+) 252 WP_061555258.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  DSM2777_RS23310 (DSM2777_23310) - 4970776..4971030 (+) 255 WP_046458213.1 Txe/YoeB family addiction module toxin -
  DSM2777_RS23315 (DSM2777_23315) ruvX 4971036..4971458 (-) 423 WP_046458214.1 Holliday junction resolvase RuvX -
  DSM2777_RS23320 (DSM2777_23320) - 4971458..4972021 (-) 564 WP_061555259.1 YqgE/AlgH family protein -
  DSM2777_RS23325 (DSM2777_23325) gshB 4972227..4973180 (-) 954 WP_061555260.1 glutathione synthase -
  DSM2777_RS23330 (DSM2777_23330) rsmE 4973190..4973921 (-) 732 WP_061555261.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DSM2777_RS23335 (DSM2777_23335) endA 4974030..4974740 (-) 711 WP_061555262.1 deoxyribonuclease I -
  DSM2777_RS23340 (DSM2777_23340) - 4974870..4975403 (-) 534 WP_061555263.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37059.45 Da        Isoelectric Point: 6.7475

>NTDB_id=172634 DSM2777_RS23300 WP_061555257.1 4969451..4970467(+) (pilT) [Obesumbacterium proteus strain DSM 2777]
MDFCDLLARSVKQNASDLHLCTGYPPVLRIDGALQTVEAEPLTSRQIDSWLAMHLPTEADTLWRQQHQVDFALNLHGGIR
LRVNAFQQLHGPSLALRIIPNTIPTLHDLSAPTALPNLLEAGSGLILVCGATGSGKSTTLAAMLNHINHHQPWHIITLED
PLEFIHIPQQSLIQQREIGAHVPSFNQALRAALREDPDVILLGELRDAESIRLALTAAETGHLVLATLHTRGAAQAVERL
IDVFPAEEKNFVRTQIAGSLRAVLAQQLLPKQGGGRVAAFEFLLNTPAVSNLIREEKAHQIATVLQTGQRQGMLSFEQDI
ARLNAQGILGRIKNDHNH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=172634 DSM2777_RS23300 WP_061555257.1 4969451..4970467(+) (pilT) [Obesumbacterium proteus strain DSM 2777]
ATGGATTTTTGTGATTTATTGGCTCGTAGTGTAAAGCAAAACGCATCAGATCTGCATCTTTGCACTGGATATCCACCCGT
TCTTCGTATCGACGGCGCATTGCAGACGGTCGAAGCAGAGCCGCTAACGTCCCGCCAGATTGACTCTTGGCTGGCGATGC
ACCTGCCTACCGAAGCCGATACGCTATGGCGACAGCAGCATCAGGTAGATTTCGCCTTAAACCTACATGGGGGCATCCGG
CTCAGAGTCAATGCTTTCCAGCAATTACATGGCCCCTCTCTGGCCCTGCGCATTATTCCAAACACGATCCCGACGCTGCA
TGATTTATCTGCGCCAACGGCACTGCCTAATCTTCTTGAGGCGGGCTCTGGGCTCATTTTGGTCTGTGGTGCAACCGGCA
GCGGAAAATCCACAACGCTTGCCGCCATGCTGAACCATATAAACCACCATCAGCCATGGCACATTATTACGCTGGAGGAC
CCTTTGGAATTTATCCATATCCCGCAGCAATCGCTTATCCAACAGCGTGAAATTGGCGCTCATGTTCCCAGCTTTAACCA
AGCGCTACGCGCCGCGCTGCGAGAGGATCCCGATGTCATTTTACTGGGTGAATTACGTGATGCGGAATCCATTCGTTTAG
CCCTCACGGCAGCAGAGACGGGCCATCTCGTATTAGCGACGCTACACACGCGAGGTGCCGCGCAAGCGGTAGAACGGCTG
ATTGATGTCTTTCCTGCCGAAGAGAAAAATTTTGTACGTACACAAATCGCAGGCAGCCTGCGGGCGGTGCTGGCACAGCA
GCTTTTGCCCAAACAGGGCGGAGGAAGAGTCGCCGCGTTTGAGTTTCTGCTCAATACACCTGCCGTCAGTAACTTAATCC
GTGAAGAAAAAGCGCACCAGATAGCTACCGTACTGCAAACGGGGCAACGACAAGGAATGCTAAGTTTTGAGCAGGATATT
GCTCGATTAAACGCACAAGGAATACTTGGGAGGATAAAAAATGACCATAACCACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.152

97.337

0.488
  pilT Vibrio cholerae strain A1552

50.152

97.337

0.488
  pilT Neisseria meningitidis 8013

49.245

97.929

0.482
  pilT Neisseria gonorrhoeae MS11

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

47.72

97.337

0.464
  pilT Acinetobacter baumannii strain A118

47.112

97.337

0.459
  pilT Acinetobacter baumannii D1279779

47.112

97.337

0.459
  pilT Acinetobacter nosocomialis M2

46.809

97.337

0.456
  pilT Legionella pneumophila strain ERS1305867

46.526

97.929

0.456
  pilT Legionella pneumophila strain Lp02

46.526

97.929

0.456
  pilT Pseudomonas stutzeri DSM 10701

46.526

97.929

0.456
  pilT Pseudomonas aeruginosa PAK

46.224

97.929

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.444

95.858

0.426
  pilU Acinetobacter baylyi ADP1

41.538

96.154

0.399
  pilU Pseudomonas stutzeri DSM 10701

39.634

97.041

0.385
  pilU Vibrio cholerae strain A1552

39.506

95.858

0.379

Multiple sequence alignment