Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AXX16_RS06535 Genome accession   NZ_CP014474
Coordinates   1417824..1418816 (+) Length   330 a.a.
NCBI ID   WP_061322351.1    Uniprot ID   -
Organism   Serratia rubidaea strain 1122     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1412824..1423816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXX16_RS06505 (AXX16_1313) hemW 1413638..1414789 (-) 1152 WP_054305190.1 radical SAM family heme chaperone HemW -
  AXX16_RS06510 (AXX16_1314) - 1414779..1415375 (-) 597 WP_015673799.1 XTP/dITP diphosphatase -
  AXX16_RS06515 (AXX16_1315) yggU 1415393..1415683 (-) 291 WP_015673798.1 DUF167 family protein YggU -
  AXX16_RS06520 (AXX16_1316) - 1415680..1416234 (-) 555 WP_015673797.1 YggT family protein -
  AXX16_RS06525 (AXX16_1317) proC 1416254..1417075 (-) 822 WP_054305191.1 pyrroline-5-carboxylate reductase -
  AXX16_RS06530 (AXX16_1318) - 1417096..1417803 (-) 708 WP_061322350.1 YggS family pyridoxal phosphate-dependent enzyme -
  AXX16_RS06535 (AXX16_1319) pilT 1417824..1418816 (+) 993 WP_061322351.1 type IV pilus twitching motility protein PilT Machinery gene
  AXX16_RS06540 (AXX16_1320) - 1418961..1419593 (+) 633 WP_071889385.1 response regulator transcription factor -
  AXX16_RS06545 (AXX16_1321) - 1419586..1423122 (+) 3537 WP_082763314.1 ATP-binding protein -
  AXX16_RS06550 (AXX16_1322) - 1423224..1423667 (+) 444 WP_071889386.1 DUF1090 domain-containing protein -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35697.08 Da        Isoelectric Point: 7.2383

>NTDB_id=171775 AXX16_RS06535 WP_061322351.1 1417824..1418816(+) (pilT) [Serratia rubidaea strain 1122]
MDIDEFVALSVKHNASDLHLCTGQPLMMRIDGELQPLAGGAVLPAEAVQRLCAALLDEPQQRQLRRAGQLDCALTTTGGT
RLRANFFYQRAGVSLALRRIAQCSPTLEQLQAPAIIPALTEREEGLILVTGATGSGKSTTLAAMIDHINRHQRRHILTLE
DPIEFIHHSALSLIQQRELGRDTRSFDAALSAALREDPDVILVGELRDSLSIRLALTAAETGHLVLATLHTRNAPQAVER
VIDVFPAEEKAYVRAQLASGLQAVIAQKLLRRAEGGRTAVFEILTATPAVSSMIREGKTHQLASVLQSGALAGMQTFAQG
MQQRIAQGIL

Nucleotide


Download         Length: 993 bp        

>NTDB_id=171775 AXX16_RS06535 WP_061322351.1 1417824..1418816(+) (pilT) [Serratia rubidaea strain 1122]
ATGGATATCGATGAATTTGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTACCGGCCAACCGTTGAT
GATGCGCATCGATGGTGAACTTCAGCCGCTGGCGGGCGGCGCCGTCCTGCCCGCCGAGGCGGTGCAGCGGCTGTGCGCGG
CGCTGCTTGATGAGCCTCAGCAGCGGCAATTACGGCGTGCCGGGCAGCTGGATTGCGCATTGACAACCACGGGCGGCACG
CGGCTGCGCGCCAACTTTTTTTATCAGCGCGCCGGCGTGTCGCTGGCGCTGCGCCGCATTGCGCAATGCAGTCCGACGCT
GGAGCAGCTGCAGGCGCCGGCGATTATCCCGGCGCTCACCGAGCGGGAGGAAGGGCTGATTCTGGTAACGGGCGCGACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATCGACCATATCAACCGTCATCAGCGGCGGCATATTCTGACGCTGGAA
GACCCGATTGAGTTTATCCACCACAGCGCGCTGTCGCTGATTCAGCAGCGCGAGCTGGGGCGCGACACCCGCAGCTTCGA
CGCGGCGCTGAGCGCCGCGCTGCGCGAAGATCCCGACGTGATTCTGGTCGGCGAGCTGCGCGACAGCCTCAGCATCCGGC
TGGCGCTGACCGCCGCGGAAACCGGCCACCTGGTGCTGGCGACCCTGCACACCCGCAACGCGCCACAGGCGGTCGAGCGG
GTGATCGACGTCTTCCCGGCGGAGGAGAAAGCCTACGTGCGCGCCCAGTTGGCCAGCGGTCTGCAGGCGGTTATCGCGCA
GAAATTACTGCGCAGGGCCGAAGGCGGCAGAACGGCGGTGTTTGAGATCCTGACGGCGACGCCGGCGGTAAGCAGCATGA
TCCGTGAAGGGAAAACGCATCAGCTGGCCAGCGTACTGCAAAGCGGTGCGCTGGCGGGCATGCAAACCTTTGCTCAGGGA
ATGCAGCAGCGTATTGCGCAGGGAATTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.727

100

0.527
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.727

100

0.527
  pilT Acinetobacter baylyi ADP1

49.697

100

0.497
  pilT Acinetobacter baumannii strain A118

49.249

100

0.497
  pilT Acinetobacter baumannii D1279779

49.249

100

0.497
  pilT Acinetobacter nosocomialis M2

48.949

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.503

100

0.491
  pilT Neisseria meningitidis 8013

48.204

100

0.488
  pilT Pseudomonas stutzeri DSM 10701

47.447

100

0.479
  pilT Pseudomonas aeruginosa PAK

46.547

100

0.47
  pilT Legionella pneumophila strain ERS1305867

46.97

100

0.47
  pilT Legionella pneumophila strain Lp02

46.97

100

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.484

96.364

0.458
  pilU Vibrio cholerae strain A1552

41.358

98.182

0.406
  pilU Pseudomonas stutzeri DSM 10701

39.818

99.697

0.397
  pilU Acinetobacter baylyi ADP1

39.319

97.879

0.385

Multiple sequence alignment