Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AV541_RS00810 Genome accession   NZ_CP014141
Coordinates   138507..139592 (+) Length   361 a.a.
NCBI ID   WP_060384024.1    Uniprot ID   -
Organism   Thermus parvatiensis strain RL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 133507..144592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AV541_RS00785 (AV541_00785) - 133571..133777 (+) 207 WP_008634012.1 cold-shock protein -
  AV541_RS00790 (AV541_00790) surE 133834..134589 (+) 756 WP_060384022.1 5'/3'-nucleotidase SurE -
  AV541_RS00795 (AV541_00795) pgeF 134586..135311 (+) 726 WP_060384023.1 peptidoglycan editing factor PgeF -
  AV541_RS00800 (AV541_00800) - 135344..135828 (+) 485 Protein_153 YqeG family HAD IIIA-type phosphatase -
  AV541_RS00805 (AV541_00805) pilB 135825..138494 (+) 2670 Protein_154 type IV pilus assembly ATPase PilB -
  AV541_RS00810 (AV541_00810) pilT 138507..139592 (+) 1086 WP_060384024.1 type IV pilus twitching motility protein PilT Machinery gene
  AV541_RS00815 (AV541_00815) gatB 139603..141012 (+) 1410 WP_060384025.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  AV541_RS00820 (AV541_00820) purM 141018..142019 (+) 1002 WP_008634005.1 phosphoribosylformylglycinamidine cyclo-ligase -
  AV541_RS00825 (AV541_00825) - 142016..142648 (+) 633 WP_008634004.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 39960.27 Da        Isoelectric Point: 7.9601

>NTDB_id=168666 AV541_RS00810 WP_060384024.1 138507..139592(+) (pilT) [Thermus parvatiensis strain RL]
MAKAPDVVDLLTLAVERGASNLVITVGLPPMIKVDGEFHPTEYEPLTPQETRRLMYALMDEKQQRIFEEEKELDFSFNLP
GKGRFRVNVFLQRGSVGGVLRVVPSAVKSFEELGLPKSIAEIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKACHIV
TIEDPIEFFHKHKRAIVNQREIGSDTKNFHRALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIVDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMIT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGAPEGARR

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=168666 AV541_RS00810 WP_060384024.1 138507..139592(+) (pilT) [Thermus parvatiensis strain RL]
ATGGCCAAAGCCCCAGACGTGGTGGACCTTTTGACGCTCGCGGTGGAACGGGGGGCGAGCAACCTCGTGATCACCGTGGG
CCTGCCCCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAACAGCGGATCTTTGAGGAGGAGAAAGAACTAGACTTCTCCTTCAACCTCCCG
GGCAAGGGGCGCTTCCGGGTGAACGTCTTCCTGCAACGGGGAAGCGTGGGCGGGGTCTTACGGGTGGTGCCGAGCGCTGT
GAAGAGCTTTGAGGAGCTCGGCCTCCCCAAGAGCATCGCCGAGATCGCCATGAGCCCAAGGGGCCTCGTCCTGGTCACGG
GGCCCACGGGAAGCGGCAAGAGCACCACGCTGGCCTCCATGATCGACTACATCAACGAACGCAAAGCCTGCCACATCGTC
ACCATCGAGGACCCCATAGAGTTTTTCCACAAGCACAAGCGGGCCATTGTGAACCAACGGGAGATCGGCTCCGACACCAA
GAACTTCCACCGGGCGCTCCGCTCGGTCCTCCGCCAGGCCCCCGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCCGCCATCACCGCCGCCGAGACGGGGCACCTGGTCATGGGCACCCTCCACACCAACTCCGCTCCCGAGACC
ATTGACCGCATCGTGGACGTCTTCCCCGAAAACCAGCAGGAGCAGGTCAGGGTGCAGCTTTCCAACAACCTGGTGGCCGT
CCTCACGCAACAGCTCCTGCCCAAGGCTTTTGGCGGCGGGCGGGTGCTGGCCTATGAGCTCATGATCGCCACCCCGGCCG
TGAGGGCCCTGATCCGGGAGGGCAAGAGCCACCAGCTAAGGAGCGTGATCCAGACCGGGGGCCAGTACGGGATGATCACC
ATGGATGCCTGCCTCGCCGATCTGTACAAGCGCAAGCTCATCACCTACGAGATGGGGCTCGCCAGGGCCGTGGACCCCAA
GGAGTTCATGAGGCTCGCGGGCGCGCCCGAGGGCGCCAGGCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.422

97.784

0.659
  pilT Pseudomonas aeruginosa PAK

54.36

95.291

0.518
  pilT Pseudomonas stutzeri DSM 10701

54.07

95.291

0.515
  pilT Acinetobacter baylyi ADP1

54.07

95.291

0.515
  pilT Acinetobacter baumannii strain A118

53.043

95.568

0.507
  pilT Acinetobacter nosocomialis M2

53.043

95.568

0.507
  pilT Acinetobacter baumannii D1279779

53.043

95.568

0.507
  pilT Neisseria meningitidis 8013

52.367

93.629

0.49
  pilT Neisseria gonorrhoeae MS11

51.632

93.352

0.482
  pilT Legionella pneumophila strain Lp02

50.581

95.291

0.482
  pilT Legionella pneumophila strain ERS1305867

50.581

95.291

0.482
  pilT Vibrio cholerae strain A1552

51.064

91.136

0.465
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.064

91.136

0.465
  pilU Pseudomonas stutzeri DSM 10701

46.356

95.014

0.44
  pilU Vibrio cholerae strain A1552

44.012

92.521

0.407
  pilU Acinetobacter baylyi ADP1

42.029

95.568

0.402

Multiple sequence alignment