Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AL539_RS09255 Genome accession   NZ_CP014053
Coordinates   1650783..1651823 (-) Length   346 a.a.
NCBI ID   WP_104975598.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FDAARGOS_108     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1645783..1656823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL539_RS09225 (AL539_17455) endA 1645927..1646622 (+) 696 WP_017635582.1 deoxyribonuclease I -
  AL539_RS09230 (AL539_17460) rsmE 1646736..1647467 (+) 732 WP_104975597.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AL539_RS09235 (AL539_17465) gshB 1647481..1648431 (+) 951 WP_005381021.1 glutathione synthase -
  AL539_RS09240 (AL539_17470) - 1648560..1649123 (+) 564 WP_104976024.1 YqgE/AlgH family protein -
  AL539_RS09245 (AL539_17475) ruvX 1649159..1649584 (+) 426 WP_053304143.1 Holliday junction resolvase RuvX -
  AL539_RS09250 (AL539_17480) pilU 1649646..1650752 (-) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AL539_RS09255 (AL539_17485) pilT 1650783..1651823 (-) 1041 WP_104975598.1 type IV pilus twitching motility protein PilT Machinery gene
  AL539_RS09260 (AL539_17490) - 1651852..1652553 (+) 702 WP_005384521.1 YggS family pyridoxal phosphate-dependent enzyme -
  AL539_RS09265 (AL539_17495) proC 1652713..1653531 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  AL539_RS09270 (AL539_17500) - 1653584..1654141 (+) 558 WP_005381012.1 YggT family protein -
  AL539_RS09275 (AL539_17505) yggU 1654141..1654431 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  AL539_RS09280 (AL539_17510) - 1654560..1654991 (+) 432 WP_104975599.1 DUF4426 domain-containing protein -
  AL539_RS09285 (AL539_17515) - 1655110..1655712 (+) 603 WP_005381009.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38531.04 Da        Isoelectric Point: 6.6198

>NTDB_id=168081 AL539_RS09255 WP_104975598.1 1650783..1651823(-) (pilT) [Vibrio alginolyticus strain FDAARGOS_108]
MDITELLNFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=168081 AL539_RS09255 WP_104975598.1 1650783..1651823(-) (pilT) [Vibrio alginolyticus strain FDAARGOS_108]
ATGGATATCACTGAGTTACTGAATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTATTTCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
TGCTTTGCGTAGTGCATTGCGCGAAGACCCAGACGTTATTCTGGTTGGTGAGATGCGTGACAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCACACCAGCTCGGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTTCCTGGTAGTGATAAAGATATGGTCCGCTCGATGCTATCTGAGTCCCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATTATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.018

96.532

0.396
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364

Multiple sequence alignment