Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AL541_RS13205 Genome accession   NZ_CP014040
Coordinates   2644963..2646069 (-) Length   368 a.a.
NCBI ID   WP_064368776.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FDAARGOS_110     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2639963..2651069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL541_RS13175 (AL541_21545) - 2640596..2641093 (+) 498 WP_104979704.1 SprT family zinc-dependent metalloprotease -
  AL541_RS13180 (AL541_21550) endA 2641244..2641939 (+) 696 WP_017635582.1 deoxyribonuclease I -
  AL541_RS13185 (AL541_21555) rsmE 2642053..2642784 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AL541_RS13190 (AL541_21560) gshB 2642798..2643748 (+) 951 WP_104979706.1 glutathione synthase -
  AL541_RS13195 (AL541_21565) - 2643877..2644440 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  AL541_RS13200 (AL541_21570) ruvX 2644476..2644901 (+) 426 WP_104979707.1 Holliday junction resolvase RuvX -
  AL541_RS13205 (AL541_21575) pilU 2644963..2646069 (-) 1107 WP_064368776.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AL541_RS13210 (AL541_21580) pilT 2646100..2647140 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  AL541_RS13215 (AL541_21585) - 2647169..2647870 (+) 702 WP_104979709.1 YggS family pyridoxal phosphate-dependent enzyme -
  AL541_RS13220 (AL541_21590) proC 2648030..2648848 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  AL541_RS13225 (AL541_21595) - 2648901..2649458 (+) 558 WP_005381012.1 YggT family protein -
  AL541_RS13230 (AL541_21600) yggU 2649458..2649748 (+) 291 WP_005381011.1 DUF167 family protein YggU -
  AL541_RS13235 (AL541_21605) - 2649876..2650307 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  AL541_RS13240 (AL541_21610) - 2650426..2651028 (+) 603 WP_104979710.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41284.39 Da        Isoelectric Point: 6.5943

>NTDB_id=167836 AL541_RS13205 WP_064368776.1 2644963..2646069(-) (pilU) [Vibrio alginolyticus strain FDAARGOS_110]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTSSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=167836 AL541_RS13205 WP_064368776.1 2644963..2646069(-) (pilU) [Vibrio alginolyticus strain FDAARGOS_110]
ATGGATCTAGATAAATTTCTCGAAGGCATGTTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTTACCGAGAGCGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCAAATTTTGCCATTGTGAGAGATTGTGGCCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACCAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCAATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGATCGGGTA
AATCGACCACCATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCATATTTTGACGGTCGAAGATCCGATC
GAATTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGCGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGTTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGCAAATGATCTGCGCTTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGAGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361

Multiple sequence alignment