Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AL515_RS20285 Genome accession   NZ_CP014030
Coordinates   4127700..4128680 (-) Length   326 a.a.
NCBI ID   WP_061068546.1    Uniprot ID   -
Organism   Citrobacter sp. FDAARGOS_156 strain FDAARGOS_155     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4122700..4133680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL515_RS20255 (AL515_19260) - 4123547..4124044 (+) 498 WP_061068543.1 SprT family zinc-dependent metalloprotease -
  AL515_RS20260 (AL515_19265) endA 4124139..4124846 (+) 708 WP_061068544.1 deoxyribonuclease I -
  AL515_RS20265 (AL515_19270) rsmE 4124921..4125652 (+) 732 WP_061068545.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AL515_RS20270 (AL515_19275) gshB 4125665..4126612 (+) 948 WP_003027083.1 glutathione synthase -
  AL515_RS20275 (AL515_19280) - 4126724..4127287 (+) 564 WP_048213499.1 YqgE/AlgH family protein -
  AL515_RS20280 (AL515_19285) ruvX 4127287..4127703 (+) 417 WP_061068855.1 Holliday junction resolvase RuvX -
  AL515_RS20285 (AL515_19290) pilT 4127700..4128680 (-) 981 WP_061068546.1 type IV pilus twitching motility protein PilT Machinery gene
  AL515_RS20290 (AL515_19295) - 4128698..4128868 (+) 171 Protein_3825 YggS family pyridoxal phosphate enzyme -
  AL515_RS20295 (AL515_19300) - 4128874..4129293 (-) 420 WP_048213194.1 DUF3828 domain-containing protein -
  AL515_RS20300 (AL515_19305) - 4129293..4129652 (-) 360 WP_061068548.1 hypothetical protein -
  AL515_RS20305 (AL515_23360) - 4130005..4130130 (-) 126 Protein_3828 type IV pili twitching motility protein PilT -
  AL515_RS20310 (AL515_19310) - 4130148..4130852 (+) 705 WP_061068549.1 YggS family pyridoxal phosphate-dependent enzyme -
  AL515_RS20315 (AL515_19315) - 4130871..4131437 (+) 567 WP_003825417.1 YggT family protein -
  AL515_RS20320 (AL515_19320) yggU 4131434..4131724 (+) 291 WP_005123286.1 DUF167 family protein YggU -
  AL515_RS20325 (AL515_19325) - 4131732..4132325 (+) 594 WP_061068550.1 XTP/dITP diphosphatase -
  AL515_RS20330 (AL515_19330) hemW 4132318..4133454 (+) 1137 WP_061068551.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36015.01 Da        Isoelectric Point: 6.2788

>NTDB_id=167598 AL515_RS20285 WP_061068546.1 4127700..4128680(-) (pilT) [Citrobacter sp. FDAARGOS_156 strain FDAARGOS_155]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRHGKIENAPFTAPDVENLLMCWLSEQQQVQWREQGQVDFAVTLTDSRRLRA
SAFAHQQGTSLALRLLPLDCPHLDDLQTPEAIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYASQRCLIQQREVGVHCVSFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAASEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=167598 AL515_RS20285 WP_061068546.1 4127700..4128680(-) (pilT) [Citrobacter sp. FDAARGOS_156 strain FDAARGOS_155]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCCGCGCG
TTGGCGCAGACATGGAAAAATCGAAAATGCGCCGTTTACCGCGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAGCAAGGGCAGGTTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGCCCATCAGCAAGGAACGTCACTGGCGCTAAGACTGCTACCGCTGGATTGTCCTCATTTAGACGATCTCCA
GACTCCCGAGGCCATACCTGAACTGTTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCCACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGACACATTCTGACGCTGGAAGATCCTATTGAA
TACCGCTACGCCAGCCAGCGTTGTCTCATTCAACAGCGGGAGGTGGGCGTACACTGCGTCTCTTTCGCCGCCGGTTTGCG
CGGTGCGTTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTAACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGCTACACACGCGTGGGGCTGCACAAGCCATTGCGCGGCTGGTGGATTCC
TTTGCTGCATCAGAGAAAGATCCGGTGCGTAATCAACTGGCAGACAGCCTGCGGGCGGTTCTTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAGCTGCTCGTTAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATACAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTGCAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368

Multiple sequence alignment