Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AL479_RS12840 Genome accession   NZ_CP014015
Coordinates   2649106..2650086 (+) Length   326 a.a.
NCBI ID   WP_061076328.1    Uniprot ID   -
Organism   Citrobacter amalonaticus strain FDAARGOS_122     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2644106..2655086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL479_RS12810 (AL479_12200) ansB 2644617..2645663 (+) 1047 WP_061076324.1 L-asparaginase 2 -
  AL479_RS12815 (AL479_12205) hemW 2645782..2646918 (-) 1137 WP_061076325.1 radical SAM family heme chaperone HemW -
  AL479_RS12820 (AL479_12210) - 2646911..2647504 (-) 594 WP_061076326.1 XTP/dITP diphosphatase -
  AL479_RS12825 (AL479_12215) yggU 2647512..2647802 (-) 291 WP_044255664.1 DUF167 family protein YggU -
  AL479_RS12830 (AL479_12220) - 2647799..2648365 (-) 567 WP_042998061.1 YggT family protein -
  AL479_RS12835 (AL479_12225) - 2648384..2649088 (-) 705 WP_061076327.1 YggS family pyridoxal phosphate-dependent enzyme -
  AL479_RS12840 (AL479_12230) pilT 2649106..2650086 (+) 981 WP_061076328.1 type IV pilus twitching motility protein PilT Machinery gene
  AL479_RS12845 (AL479_12235) ruvX 2650100..2650516 (-) 417 WP_061077975.1 Holliday junction resolvase RuvX -
  AL479_RS12850 (AL479_12240) - 2650516..2651079 (-) 564 WP_061076329.1 YqgE/AlgH family protein -
  AL479_RS12855 (AL479_12245) gshB 2651254..2652201 (-) 948 WP_061076330.1 glutathione synthase -
  AL479_RS12860 (AL479_12250) rsmE 2652214..2652945 (-) 732 WP_061076331.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AL479_RS12865 (AL479_12255) endA 2653025..2653732 (-) 708 WP_061076332.1 deoxyribonuclease I -
  AL479_RS12870 (AL479_12260) - 2653824..2654324 (-) 501 WP_061076333.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35662.70 Da        Isoelectric Point: 6.8299

>NTDB_id=167218 AL479_RS12840 WP_061076328.1 2649106..2650086(+) (pilT) [Citrobacter amalonaticus strain FDAARGOS_122]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGGMERAPFTVADITGLLADWLDEGQQAALRQNGQLDFAVSLGDNQRLRA
SAFHQRQGTSLALRLLPTQCPTLETLGTPSVLPELLRSENGLILVTGATGSGKSTTLAAMVAWLNQHIDGHILTLEDPIE
YVYASQRCLIQQREVGLHCTSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLESDKQEGRVALYELLINTPAAGNLIREGKSHQLPHVIQTGQQMGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=167218 AL479_RS12840 WP_061076328.1 2649106..2650086(+) (pilT) [Citrobacter amalonaticus strain FDAARGOS_122]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGCAAACGGGGGGGCATGGAGCGTGCGCCCTTCACCGTAGCCGACATCACCGGACTGCTGGCGGACTGGCTGGATG
AGGGGCAGCAGGCGGCGTTACGGCAAAACGGGCAACTGGATTTCGCCGTTTCGCTTGGGGATAACCAGCGCCTGCGGGCC
AGCGCCTTTCACCAGCGGCAGGGGACGTCGCTGGCGCTGCGGCTGCTGCCGACGCAGTGCCCGACGCTGGAAACGCTGGG
GACGCCATCCGTACTGCCCGAACTGCTCCGCAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGCAGCGGAAAAT
CAACCACCCTGGCGGCGATGGTCGCGTGGCTGAATCAGCATATCGATGGACATATTTTGACGCTGGAAGATCCCATCGAG
TATGTCTACGCCAGCCAGCGCTGCCTGATTCAGCAGCGTGAAGTGGGACTGCACTGCACCTCATTTGCCGCCGGATTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGAGAGTTACGCGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGTGCCGCGCAGGCAATCGAGCGACTGATTGATTCT
TTTCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCAGGCAGTCTGCGCGCCGTGCTGTCGCAGAAACTGGAGTC
CGACAAGCAGGAGGGGCGCGTTGCGCTGTATGAACTGCTGATCAACACCCCGGCGGCGGGGAATTTGATTCGGGAAGGGA
AGTCCCATCAGTTACCCCATGTCATTCAGACCGGGCAGCAGATGGGGATGATGACCTTTGCCCAAAGCCTGCAACAGCGA
CAGGCGCAAGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.75

98.16

0.429
  pilU Vibrio cholerae strain A1552

43.548

95.092

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383

Multiple sequence alignment