Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   APT61_RS03980 Genome accession   NZ_CP013990
Coordinates   832340..833317 (+) Length   325 a.a.
NCBI ID   WP_059307230.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain USDA-ARS-USMARC-60222     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 827340..838317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APT61_RS03950 (APT61_03950) - 828582..828944 (-) 363 WP_059307226.1 endonuclease domain-containing protein -
  APT61_RS03955 (APT61_03955) hemW 829008..830150 (-) 1143 WP_059307227.1 radical SAM family heme chaperone HemW -
  APT61_RS03960 (APT61_03960) - 830143..830736 (-) 594 WP_059307228.1 XTP/dITP diphosphatase -
  APT61_RS03965 (APT61_03965) yggU 830740..831036 (-) 297 WP_032613939.1 DUF167 family protein YggU -
  APT61_RS03970 (APT61_03970) - 831033..831599 (-) 567 WP_032613937.1 YggT family protein -
  APT61_RS03975 (APT61_03975) - 831621..832322 (-) 702 WP_059307229.1 YggS family pyridoxal phosphate-dependent enzyme -
  APT61_RS03980 (APT61_03980) pilT 832340..833317 (+) 978 WP_059307230.1 type IV pilus twitching motility protein PilT Machinery gene
  APT61_RS03985 (APT61_03985) - 833417..834106 (+) 690 WP_032613957.1 IclR family transcriptional regulator domain-containing protein -
  APT61_RS03990 (APT61_03990) ruvX 834145..834561 (-) 417 WP_032613930.1 Holliday junction resolvase RuvX -
  APT61_RS03995 (APT61_03995) - 834561..835121 (-) 561 WP_032613928.1 YqgE/AlgH family protein -
  APT61_RS04000 (APT61_04000) gshB 835213..836160 (-) 948 WP_059307231.1 glutathione synthase -
  APT61_RS04005 (APT61_04005) rsmE 836180..836911 (-) 732 WP_032613955.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  APT61_RS04010 (APT61_04010) endA 837023..837730 (-) 708 WP_032613924.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35671.82 Da        Isoelectric Point: 6.7692

>NTDB_id=166945 APT61_RS03980 WP_059307230.1 832340..833317(+) (pilT) [Leclercia adecarboxylata strain USDA-ARS-USMARC-60222]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMELAPFPGPDVVVLLKSWLSDEQQGAWCAQGQVDFAVTLESHRLRAS
AFAHTQGCSLALRLLPEQCPQLHSLGTPRAIPELLTRDNGLILVTGATSSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNQQGRVALFELLINTPAAANLIREGKTYQLPGVIQTGLQAGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=166945 APT61_RS03980 WP_059307230.1 832340..833317(+) (pilT) [Leclercia adecarboxylata strain USDA-ARS-USMARC-60222]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACGAGGAAAGATGGAATTGGCGCCTTTTCCGGGGCCGGATGTGGTGGTGCTGCTGAAAAGCTGGCTCAGCG
ATGAACAACAGGGGGCATGGTGTGCTCAGGGGCAGGTCGATTTTGCCGTCACTCTGGAGAGCCACCGGCTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCAGAACAGTGCCCGCAATTGCATAGTCTGGGTAC
GCCGCGGGCGATCCCCGAGCTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGGGCCACCAGCAGCGGCAAGTCCA
CCACGCTTGCGGCAATGGTCGACTTTCTTAATCACCACAGCGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGTAACGCCTTTGCCGATGCGCTGCGGGC
GGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAACTGCGCGATAGCGAAACGATCCGCCTTGCCCTCACCGCCG
CAGAGACCGGGCATCTGGTACTGGCGACCCTGCATACCCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAACTGGCCGGCAGTTTGCAGGCGGTGCTGGCGCAAAAACTCGAAAGGGA
CAACCAGCAGGGGCGCGTCGCGCTGTTTGAGTTACTCATTAACACCCCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGCGTGATCCAGACCGGGCTGCAGGCAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.489
  pilT Vibrio cholerae strain A1552

48.624

100

0.489
  pilT Legionella pneumophila strain Lp02

47.826

99.077

0.474
  pilT Legionella pneumophila strain ERS1305867

47.826

99.077

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.265

97.538

0.471
  pilT Acinetobacter baylyi ADP1

46.789

100

0.471
  pilT Neisseria meningitidis 8013

46.646

100

0.471
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.468
  pilT Pseudomonas aeruginosa PAK

47.634

97.538

0.465
  pilT Acinetobacter baumannii D1279779

47.335

98.154

0.465
  pilT Acinetobacter baumannii strain A118

47.335

98.154

0.465
  pilT Acinetobacter nosocomialis M2

47.335

98.154

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.312

96.615

0.418
  pilU Vibrio cholerae strain A1552

39.937

97.846

0.391
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.382
  pilU Acinetobacter baylyi ADP1

36.42

99.692

0.363

Multiple sequence alignment