Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AUC44_RS01050 Genome accession   NZ_CP013910
Coordinates   217744..219018 (-) Length   424 a.a.
NCBI ID   WP_062157007.1    Uniprot ID   -
Organism   Deinococcus actinosclerus strain BM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 212744..224018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AUC44_RS01040 (AUC44_01030) - 213305..214606 (+) 1302 WP_062157005.1 thymidine phosphorylase -
  AUC44_RS01045 (AUC44_01035) - 214689..217367 (-) 2679 WP_082688896.1 DUF11 domain-containing protein -
  AUC44_RS01050 (AUC44_01040) pilT 217744..219018 (-) 1275 WP_062157007.1 type IV pilus twitching motility protein PilT Machinery gene
  AUC44_RS01055 (AUC44_01045) pilB 219061..221739 (-) 2679 WP_062157008.1 type II/IV secretion system protein Machinery gene
  AUC44_RS01060 (AUC44_01050) - 221803..222330 (-) 528 WP_062157009.1 YqeG family HAD IIIA-type phosphatase -
  AUC44_RS01065 (AUC44_01055) pgeF 222464..223192 (-) 729 WP_231724495.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 45926.20 Da        Isoelectric Point: 7.0810

>NTDB_id=165971 AUC44_RS01050 WP_062157007.1 217744..219018(-) (pilT) [Deinococcus actinosclerus strain BM2]
MTQPAADITDILRFAADKGASDVIITVGLAPQFKLQGVYDSQGFTELAPTDTRKLMYSMMNEKQQRTFEERRELDFSFAL
GEKARFRVNAFMQRGNVGGVLRLIPTKIKSAQEMGLPANVIEIANAPRGLVLVTGPTGSGKSTTLAAMIDHINTTKRLHI
MTIEDPIEFMHTHKQSIINQREVGADTMSFNDALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRLDGQGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMV
TMDAFLANLYRRRVISFDTGVERAVDSKEFARLANDPSIATAGGAASMPAGYGQAPVQGFGATVTPAQGGYASGRSDFGR
GNSSGDARTTSTPETNGGGGYGRR

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=165971 AUC44_RS01050 WP_062157007.1 217744..219018(-) (pilT) [Deinococcus actinosclerus strain BM2]
ATGACCCAGCCTGCCGCCGACATCACCGACATCCTGCGTTTCGCCGCCGACAAGGGCGCCTCCGACGTGATCATCACGGT
CGGGCTCGCGCCGCAGTTCAAGCTGCAGGGCGTATACGACTCGCAGGGCTTCACGGAGCTCGCCCCGACCGACACCCGCA
AACTGATGTACTCCATGATGAACGAGAAGCAGCAGCGGACCTTCGAGGAACGCCGCGAGCTGGACTTCTCGTTCGCGCTG
GGCGAGAAGGCCCGCTTCCGCGTGAACGCCTTCATGCAGCGCGGCAACGTGGGCGGCGTGCTGCGCCTGATTCCCACGAA
GATCAAGAGCGCCCAGGAGATGGGCCTGCCGGCCAACGTCATCGAGATCGCCAACGCCCCGCGCGGCCTGGTGCTCGTGA
CCGGCCCGACCGGCTCGGGCAAGTCCACGACGCTCGCGGCGATGATCGACCACATCAACACCACCAAGCGGCTGCACATC
ATGACCATCGAGGATCCCATCGAGTTCATGCACACGCACAAGCAGTCGATCATCAACCAGCGTGAGGTCGGCGCGGACAC
CATGAGCTTCAACGACGCGCTGCGCGCCGTGCTGCGTCAGGCGCCCGACGTGATCCTGGTGGGCGAAATGCGCGACTACG
AGACCATCAAGGCCGCCGTGACCGCCGCCGAGACCGGGCACCTCGTGATGGGCACCCTGCACACGAACAGTGCTCCGGAA
TCCATCGACCGTATCGTGGACGTGTTCCCCGAAGAGCAGCAGGAGCAGATCCGCGTGCAGCTCGCGAACAACCTCGTGGC
GGTCATGACGCAGCAGCTGCTGCCGCGCCTGGACGGGCAAGGCCGCATCCTGGCGTACGAGCTGCTCATCGCCAACCCGG
CCGTGCGCGCCCTGATCCGCGAGGGCAAGACGTACCAGATCACGTCCGTCATGCAGACCGGCGCCCGCGAGGGCATGGTC
ACCATGGACGCCTTCCTGGCGAACCTGTACCGCCGCCGCGTGATCTCCTTCGACACCGGTGTCGAGCGCGCCGTGGACAG
CAAGGAATTCGCCCGACTCGCGAACGACCCCAGCATCGCCACGGCGGGCGGCGCGGCCAGCATGCCCGCCGGGTACGGCC
AGGCGCCCGTGCAGGGCTTCGGCGCGACCGTCACCCCCGCCCAGGGCGGCTACGCCTCGGGCCGCAGCGACTTCGGGCGC
GGCAACAGCAGCGGGGACGCCCGCACCACCAGCACCCCCGAAACGAACGGCGGCGGAGGCTACGGCCGGCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

75.355

99.528

0.75
  pilT Neisseria meningitidis 8013

50.862

82.075

0.417
  pilT Pseudomonas stutzeri DSM 10701

50.581

81.132

0.41
  pilT Neisseria gonorrhoeae MS11

50.581

81.132

0.41
  pilT Pseudomonas aeruginosa PAK

50

81.132

0.406
  pilT Acinetobacter baumannii strain A118

52.121

77.83

0.406
  pilT Acinetobacter baumannii D1279779

52.121

77.83

0.406
  pilT Acinetobacter nosocomialis M2

52.121

77.83

0.406
  pilT Acinetobacter baylyi ADP1

50.737

79.953

0.406
  pilT Legionella pneumophila strain ERS1305867

49.128

81.132

0.399
  pilT Legionella pneumophila strain Lp02

49.128

81.132

0.399
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

77.594

0.382
  pilT Vibrio cholerae strain A1552

49.24

77.594

0.382
  pilU Acinetobacter baylyi ADP1

44.253

82.075

0.363
  pilU Pseudomonas stutzeri DSM 10701

44.606

80.896

0.361

Multiple sequence alignment