Detailed information NTG0165972

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: AUC44_RS01055
• Genome accession: NZ_CP013910
• Coordinates: 219061..221739 (-)
• Length: 892 a.a.
• NCBI ID: WP_062157008.1
• Uniprot ID: -
• Organism: Deinococcus actinosclerus strain BM2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 214061..226739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AUC44_RS01045 (AUC44_01035)	-	214689..217367 (-)	2679	WP_082688896.1	DUF11 domain-containing protein	-
AUC44_RS01050 (AUC44_01040)	pilT	217744..219018 (-)	1275	WP_062157007.1	type IV pilus twitching motility protein PilT	Machinery gene
AUC44_RS01055 (AUC44_01045)	pilB	219061..221739 (-)	2679	WP_062157008.1	type II/IV secretion system protein	Machinery gene
AUC44_RS01060 (AUC44_01050)	-	221803..222330 (-)	528	WP_062157009.1	YqeG family HAD IIIA-type phosphatase	-
AUC44_RS01065 (AUC44_01055)	pgeF	222464..223192 (-)	729	WP_231724495.1	peptidoglycan editing factor PgeF	-
AUC44_RS01070 (AUC44_01060)	-	223373..224167 (+)	795	WP_062157011.1	enoyl-ACP reductase FabI	-
AUC44_RS01075 (AUC44_01065)	-	224221..224574 (-)	354	WP_062157012.1	winged helix-turn-helix transcriptional regulator	-
AUC44_RS01080 (AUC44_01070)	-	224753..225706 (+)	954	WP_197408561.1	NADP-dependent oxidoreductase	-
AUC44_RS01085 (AUC44_01075)	-	225786..226199 (+)	414	WP_062157013.1	isochorismatase family protein	-

Sequence

Protein

• Length: 892 a.a.
• Molecular weight: 97534.54 Da
• Isoelectric Point: 5.0666

>NTDB_id=165972 AUC44_RS01055 WP_062157008.1 219061..221739(-) (pilB) [Deinococcus actinosclerus strain BM2]
MALSIGDRRLGAILLEQGYVNDTDLQKALVRHAEVGGRLAEILIDSGLVGEKRIARAIEEALGIPLVNLLVVNPEPGALQ
AVRAQTALQHQAFPFALDGQTLRVAIVDPLSSMAIEALEDDSGLNIEVYQALRDQIMWAIATYYPELHLTAELPSEAEGG
PAGGMLGQRLIGRGLITDAQLQVALDAQQQTGEPLGSTLIAQRIISEDQLYEVLAEQTNAVFLRNPRDFQPSEDVLGSML
RADALRLTAVPVDETDHGVTVVASDPRKREDIEALVGRPVQIVLAKPRDIETLIERFYPQRGRLGEQMVQQGTLSREQLR
EALQVQAREGRVKPLGEVIVELGFAAADEIDSALQKQNSGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPVQNPPD
NKVALMIPETTARRYGVVPVRLQGESLVVAMKDPRNVFALDDLKLITGREIIPAVMAEKDITRLIERYFGSKDMANLNQQ
LAKESKERENANKRQDAEDLSAGLDDNAVVRVVDNIIREAALQEASDIHVEPTETALKVRYRVDGILREQNELPKGSSQS
ILARIKIMGHLDISERRIPQDGRIRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSEHNYQRYLDTIH
KPNGIFLVTGPTGSGKSFTSFSTLKRIAVPEKNTTTIEDPVEYEIPGIIQSQVNNAAGMTFARALRAFLRQDPDIIFVGE
IRDTETAKIAVEAALTGHLVLATLHTNDAPGAIVRLEEMGVEHFNIGAAVVGVVAQRLVRKVCPDCRAPTNADPDVLRRL
GITERDLRGAQLMRGAGCNRCGGTGYKGRMGIHELMVIDEALRVAIGSGKNATEITEVAVNQSGMKTLRQDGIEKALKGI
TTLEEVLAVTSK

Nucleotide

• Length: 2679 bp

>NTDB_id=165972 AUC44_RS01055 WP_062157008.1 219061..221739(-) (pilB) [Deinococcus actinosclerus strain BM2]
ATGGCTCTTTCCATCGGTGACCGGCGCCTGGGCGCCATCCTGCTCGAACAGGGGTACGTGAACGACACGGACCTGCAAAA
AGCCCTCGTCCGTCACGCCGAGGTCGGCGGCCGACTCGCGGAGATCCTGATCGACTCCGGCCTGGTGGGCGAGAAACGCA
TCGCGCGCGCCATCGAGGAGGCGCTAGGCATCCCGCTCGTGAACCTGCTGGTCGTGAATCCCGAACCCGGCGCGCTGCAG
GCCGTGCGGGCGCAGACGGCCCTGCAACACCAGGCGTTCCCGTTCGCGCTGGACGGGCAGACGCTGCGCGTGGCGATCGT
CGATCCGCTCTCGAGCATGGCGATCGAGGCGCTGGAGGACGACAGCGGCCTGAACATCGAGGTGTATCAGGCGCTGCGTG
ACCAGATCATGTGGGCCATCGCCACGTACTACCCCGAACTGCATCTGACGGCCGAGCTGCCCAGCGAGGCCGAGGGCGGC
CCGGCGGGCGGGATGCTGGGGCAGCGCCTGATCGGGCGCGGCCTGATCACCGACGCGCAGCTGCAGGTGGCGCTCGACGC
GCAGCAGCAGACCGGCGAGCCGCTGGGCAGCACCCTGATCGCGCAGCGGATCATCAGTGAGGATCAGCTGTACGAGGTGC
TGGCCGAGCAGACCAACGCGGTGTTCCTGCGCAACCCGCGGGATTTCCAGCCCAGCGAGGACGTGCTGGGCAGCATGCTG
CGCGCCGACGCGCTGCGCCTGACGGCGGTACCGGTGGATGAAACCGATCACGGCGTGACCGTGGTTGCCAGCGACCCCCG
CAAACGCGAGGACATCGAGGCGCTGGTGGGCCGCCCCGTACAGATCGTGCTGGCCAAACCGCGCGACATCGAGACGCTGA
TCGAGCGCTTCTACCCGCAGCGTGGGCGGCTGGGCGAGCAGATGGTGCAGCAGGGCACCCTGTCGCGCGAGCAGCTGCGC
GAGGCGCTGCAGGTGCAGGCCCGCGAGGGCCGCGTCAAGCCGCTGGGCGAGGTCATCGTGGAGCTGGGCTTCGCGGCCGC
GGACGAGATCGACAGCGCCCTGCAGAAACAGAACTCCGGGGGCGGACGCCTGGAAGACACGCTGGTGCAGTCCGGGAAAC
TCAGCCCGGAGATGCTGGCGCGCTCCCTGGCCGCGCAGCTCGGCTACGAGTTCCTGGACCCGGTTCAGAACCCACCGGAC
AACAAGGTCGCGCTGATGATCCCCGAGACGACCGCCCGCCGCTACGGCGTGGTCCCGGTCCGGTTGCAGGGCGAATCGCT
GGTCGTGGCGATGAAGGACCCGCGCAACGTGTTCGCGCTGGACGACCTGAAGCTGATCACCGGGCGCGAGATCATCCCGG
CGGTCATGGCGGAAAAGGACATCACCCGCCTGATCGAGCGGTACTTCGGCAGCAAGGACATGGCGAACCTGAACCAGCAG
CTCGCCAAGGAGAGCAAGGAGCGCGAGAACGCCAACAAGCGCCAGGACGCCGAGGACCTGTCGGCCGGGCTGGACGACAA
CGCCGTGGTGCGCGTGGTGGACAACATCATCCGCGAGGCGGCCCTCCAGGAGGCCAGCGACATCCACGTCGAGCCGACCG
AGACGGCCCTGAAGGTGCGCTACCGGGTCGACGGCATCCTGCGCGAGCAGAACGAGCTGCCCAAGGGCAGTTCGCAGAGC
ATCCTGGCGCGCATCAAGATCATGGGGCACCTGGATATCAGCGAGCGCCGCATCCCCCAGGACGGCCGCATCCGCTTCAA
GAAGGGCAGCATCGACCTCGACCTGCGACTCTCCACGCTGCCCACCGTGTACGGCGAGAAGGCTGTGATGCGTCTGCTGC
AGAAGGCCAGCAACATCCCCGAGGTCGAGCAGCTGGGCTTTTCCGAGCACAACTACCAGCGTTACCTGGACACCATCCAC
AAGCCCAACGGCATCTTCCTGGTGACCGGCCCCACCGGCTCGGGGAAATCCTTCACGTCGTTCTCGACCCTCAAGCGCAT
CGCGGTGCCCGAGAAGAACACCACCACCATCGAGGACCCGGTCGAATACGAGATTCCGGGCATCATCCAGTCGCAGGTGA
ACAACGCCGCAGGCATGACCTTCGCCCGCGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCTTCGTGGGCGAG
ATCCGCGACACCGAGACCGCCAAAATCGCCGTGGAGGCCGCGCTGACCGGTCACCTCGTGCTGGCTACGCTGCACACCAA
CGACGCGCCGGGCGCCATCGTGCGTCTGGAGGAGATGGGCGTCGAGCACTTCAACATCGGCGCGGCCGTGGTGGGCGTGG
TCGCGCAGCGACTCGTGCGCAAGGTCTGCCCGGACTGCAGGGCCCCCACGAACGCCGACCCGGACGTCCTGCGCCGCCTC
GGCATCACCGAGCGGGACCTGCGCGGCGCGCAGCTCATGCGCGGCGCGGGCTGCAACCGCTGCGGCGGCACCGGCTACAA
GGGCCGCATGGGCATCCACGAGCTGATGGTGATCGACGAGGCGCTGCGCGTCGCCATCGGCTCCGGGAAGAACGCCACCG
AGATCACCGAGGTCGCCGTCAACCAGAGTGGCATGAAGACCCTGCGGCAGGACGGCATCGAGAAGGCACTCAAGGGGATC
ACCACCCTGGAAGAGGTCCTGGCCGTCACCAGCAAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (516-781)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (368-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (199-302)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	78.139	100	0.781
pilF	Thermus thermophilus HB27	54.525	100	0.547