Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FORC25_RS10350 Genome accession   NZ_CP013101
Coordinates   2347129..2348181 (-) Length   350 a.a.
NCBI ID   WP_003451114.1    Uniprot ID   A0A173YF66
Organism   Clostridium perfringens strain FORC_025     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2342129..2353181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC25_RS10325 (FORC25_2121) sigE 2342722..2343429 (-) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -
  FORC25_RS10330 (FORC25_2122) spoIIGA 2343443..2344249 (-) 807 WP_003458523.1 sigma-E processing peptidase SpoIIGA -
  FORC25_RS10340 (FORC25_2124) ftsZ 2344654..2345799 (-) 1146 WP_003449411.1 cell division protein FtsZ -
  FORC25_RS10345 (FORC25_2125) - 2345813..2347051 (-) 1239 WP_003481817.1 cell division FtsA domain-containing protein -
  FORC25_RS10350 (FORC25_2126) pilT 2347129..2348181 (-) 1053 WP_003451114.1 type IV pilus twitching motility protein PilT Machinery gene
  FORC25_RS10355 (FORC25_2127) sigK 2348362..2349063 (-) 702 WP_003451260.1 RNA polymerase sporulation sigma factor SigK -
  FORC25_RS10360 (FORC25_2128) - 2349126..2350793 (-) 1668 WP_011010596.1 penicillin-binding transpeptidase domain-containing protein -
  FORC25_RS10365 (FORC25_2129) udk 2350906..2351532 (-) 627 WP_003459577.1 uridine kinase -
  FORC25_RS10370 (FORC25_2130) - 2351532..2352761 (-) 1230 WP_070957009.1 peptidase U32 family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39029.73 Da        Isoelectric Point: 5.4480

>NTDB_id=159894 FORC25_RS10350 WP_003451114.1 2347129..2348181(-) (pilT) [Clostridium perfringens strain FORC_025]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=159894 FORC25_RS10350 WP_003451114.1 2347129..2348181(-) (pilT) [Clostridium perfringens strain FORC_025]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCGTATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTTTAGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATCTTAAAAGAGGACTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCAGCTTTAATCAATGAAATAAATAGTAATAGAGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATAGGCAAGGACACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCTCTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCGGCAGAGACTGGACACTTAGTATTTTCAACTCTTCATACTATAGGAGCTGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAGCAGGAACAAATAAAAATACAGTTAGCTTCTGTTTTACAAATAATTATATCACAACA
ATTAGTGGAAACCGTTGATGGAGATAGAAATGCTGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCTTCTATCCAAACTGGAAGTAAATATGGAATGAGAACGATGGACATGGAACTT
GCTAATCTGTATAGAGAAGGTATAATAACACAAGAAACGGCAATGAATTCTGCTATAGATAGGGAGATTTTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A173YF66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.857

0.491
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Pseudomonas aeruginosa PAK

50.298

96

0.483
  pilT Legionella pneumophila strain Lp02

49.415

97.714

0.483
  pilT Legionella pneumophila strain ERS1305867

49.415

97.714

0.483
  pilT Pseudomonas stutzeri DSM 10701

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.602

94.857

0.48
  pilT Acinetobacter nosocomialis M2

49.702

96

0.477
  pilT Acinetobacter baumannii D1279779

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.702

96

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.653

96.286

0.391
  pilU Acinetobacter baylyi ADP1

39.823

96.857

0.386

Multiple sequence alignment