Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AP033_RS05460 Genome accession   NZ_CP013014
Coordinates   9312..10349 (+) Length   345 a.a.
NCBI ID   WP_000350196.1    Uniprot ID   A0A0K9UNT8
Organism   Vibrio cholerae strain Env-390     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4312..15349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AP033_RS05425 (AP033_20005) hemW 4486..5661 (-) 1176 WP_000957347.1 radical SAM family heme chaperone HemW -
  AP033_RS05430 (AP033_20006) - 5655..6257 (-) 603 WP_000725015.1 XTP/dITP diphosphatase -
  AP033_RS05435 (AP033_20007) - 6327..6758 (-) 432 WP_001233674.1 DUF4426 domain-containing protein -
  AP033_RS05440 (AP033_20008) yggU 6804..7094 (-) 291 WP_000741915.1 DUF167 family protein YggU -
  AP033_RS05445 (AP033_20009) - 7094..7651 (-) 558 WP_001087261.1 YggT family protein -
  AP033_RS05450 (AP033_20010) proC 7705..8523 (-) 819 WP_032476070.1 pyrroline-5-carboxylate reductase -
  AP033_RS05455 (AP033_20011) - 8576..9286 (-) 711 WP_001256655.1 YggS family pyridoxal phosphate-dependent enzyme -
  AP033_RS05460 (AP033_20012) pilT 9312..10349 (+) 1038 WP_000350196.1 type IV pilus twitching motility protein PilT Machinery gene
  AP033_RS05465 (AP033_20013) pilU 10363..11469 (+) 1107 WP_000422573.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AP033_RS05470 (AP033_20014) - 11587..12384 (+) 798 WP_001123215.1 helix-turn-helix transcriptional regulator -
  AP033_RS05475 (AP033_20015) tyrS 12493..13774 (+) 1282 Protein_15 tyrosine--tRNA ligase -
  AP033_RS05480 (AP033_20016) ruvX 13812..14234 (-) 423 WP_000091868.1 Holliday junction resolvase RuvX -
  AP033_RS05485 (AP033_20017) - 14315..14878 (-) 564 WP_001054771.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38249.86 Da        Isoelectric Point: 6.6672

>NTDB_id=159184 AP033_RS05460 WP_000350196.1 9312..10349(+) (pilT) [Vibrio cholerae strain Env-390]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=159184 AP033_RS05460 WP_000350196.1 9312..10349(+) (pilT) [Vibrio cholerae strain Env-390]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTACCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGTTAGA
GCAACTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAGCGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTTTGGTTGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
GCTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCGACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATGGCGCGTGGTGTGGTGGATGCGCAAGAAGTGCAGAGCAAAATCGAGTTGGACTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K9UNT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.71

100

0.997
  pilT Vibrio cholerae strain A1552

99.71

100

0.997
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment