Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AO703_RS17560 Genome accession   NZ_CP012871
Coordinates   3788629..3789609 (-) Length   326 a.a.
NCBI ID   WP_062741934.1    Uniprot ID   A0A806X7X2
Organism   [Enterobacter] lignolyticus strain G5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3783629..3794609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO703_RS17530 (AO703_17480) endA 3784002..3784709 (+) 708 WP_062741925.1 deoxyribonuclease I -
  AO703_RS17535 (AO703_17485) rsmE 3784755..3785486 (+) 732 WP_013364837.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AO703_RS17540 (AO703_17490) gshB 3785499..3786446 (+) 948 WP_013364836.1 glutathione synthase -
  AO703_RS17545 (AO703_17495) - 3786545..3787108 (+) 564 WP_062741928.1 YqgE/AlgH family protein -
  AO703_RS17550 (AO703_17500) ruvX 3787108..3787524 (+) 417 WP_062741930.1 Holliday junction resolvase RuvX -
  AO703_RS17555 (AO703_17505) - 3787565..3788218 (-) 654 WP_062741932.1 LuxR C-terminal-related transcriptional regulator -
  AO703_RS17560 (AO703_17510) pilT 3788629..3789609 (-) 981 WP_062741934.1 type IV pilus twitching motility protein PilT Machinery gene
  AO703_RS17565 (AO703_17515) - 3789625..3790329 (+) 705 WP_044612060.1 YggS family pyridoxal phosphate-dependent enzyme -
  AO703_RS17570 (AO703_17520) - 3790348..3790914 (+) 567 WP_013364830.1 YggT family protein -
  AO703_RS17575 (AO703_17525) yggU 3790911..3791201 (+) 291 WP_062741936.1 DUF167 family protein YggU -
  AO703_RS17580 (AO703_17530) - 3791210..3791803 (+) 594 WP_062741938.1 XTP/dITP diphosphatase -
  AO703_RS17585 (AO703_17535) hemW 3791807..3792940 (+) 1134 WP_062741940.1 radical SAM family heme chaperone HemW -
  AO703_RS17590 (AO703_17540) ansB 3792988..3794034 (-) 1047 WP_013364826.1 L-asparaginase 2 -
  AO703_RS23495 - 3794262..3794429 (+) 168 WP_418112728.1 DUF559 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35103.17 Da        Isoelectric Point: 7.0397

>NTDB_id=157970 AO703_RS17560 WP_062741934.1 3788629..3789609(-) (pilT) [[Enterobacter] lignolyticus strain G5]
MDMQEIVSLSVKHNVSDLHLCSCMQARWRREGRLEPAPFPSPDLPMLLDGWLNAAQQRQWREDRQVDFAVDAGGGQRLRG
NASVHCRGVSVVLRLLPVSCPQLSTLGAPAAIPSLLDAESGIILVTGATGSGKSTTLAAMVDSLNQQRDGHILTLEDPVE
FVHHSARCLIEQREVGLHCASYAAALRSALRQDPDVLLLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAVERLIDS
FPAGEKETVRSQLAGSLCAVLAQKLLPGRQGGRVALYELLINTAATASLIREGKTHQLAGVMQTGQQAGMQTYAQSHQQR
IAEGKM

Nucleotide


Download         Length: 981 bp        

>NTDB_id=157970 AO703_RS17560 WP_062741934.1 3788629..3789609(-) (pilT) [[Enterobacter] lignolyticus strain G5]
ATGGATATGCAGGAAATTGTGTCCCTTAGTGTAAAGCATAACGTTTCGGATCTACACCTGTGTAGTTGCATGCAGGCGCG
CTGGCGGCGAGAAGGGCGTCTCGAGCCCGCGCCTTTTCCGTCGCCGGATCTCCCTATGCTGCTCGACGGCTGGCTTAATG
CGGCGCAGCAGCGGCAGTGGCGCGAGGATCGCCAGGTGGATTTTGCCGTGGATGCCGGGGGCGGTCAGCGCCTTCGCGGC
AATGCGTCTGTGCACTGCCGGGGTGTTTCGGTGGTTCTGCGCCTGCTGCCGGTCTCCTGCCCGCAGCTTTCGACGCTGGG
GGCGCCTGCGGCGATCCCTTCGCTGCTCGACGCCGAGAGCGGGATTATTCTGGTGACCGGCGCGACCGGCAGCGGCAAGT
CCACGACGCTTGCCGCGATGGTGGATTCTCTTAACCAGCAGCGGGACGGGCATATTCTCACGCTTGAAGATCCGGTGGAG
TTTGTCCATCACAGCGCGCGGTGTCTGATTGAGCAACGGGAGGTGGGTTTGCACTGCGCGTCCTATGCCGCCGCGCTGAG
AAGCGCGCTGCGTCAGGACCCGGATGTTCTGCTGCTGGGAGAGCTGCGTGATGCTGAAACTATCCGTCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTACTGGCGACCCTGCACACCCGCGGCGCGGCGCAGGCCGTGGAGCGGCTCATTGACAGC
TTCCCTGCCGGGGAAAAAGAGACCGTCCGCAGCCAGCTTGCCGGCAGCCTGTGCGCGGTGCTGGCGCAGAAGCTGCTGCC
TGGCAGGCAGGGGGGAAGGGTGGCGCTGTATGAGCTGCTGATCAACACCGCGGCGACCGCCAGCTTGATTCGCGAGGGGA
AAACGCACCAGCTGGCAGGCGTGATGCAGACAGGGCAGCAGGCCGGGATGCAAACCTACGCCCAAAGCCATCAGCAGCGG
ATTGCCGAGGGAAAAATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A806X7X2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

100

0.503
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.987

96.933

0.426
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.066

100

0.386
  pilU Acinetobacter baylyi ADP1

37.072

98.466

0.365

Multiple sequence alignment