Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AO353_RS08155 Genome accession   NZ_CP012830
Coordinates   1798387..1799421 (-) Length   344 a.a.
NCBI ID   WP_054594459.1    Uniprot ID   A0A2N8C5V9
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1793387..1804421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS08125 (AO353_08125) ruvX 1793565..1794002 (+) 438 WP_054594455.1 Holliday junction resolvase RuvX -
  AO353_RS08130 (AO353_08130) pyrR 1794098..1794604 (+) 507 WP_054594456.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  AO353_RS08135 (AO353_08135) - 1794631..1795635 (+) 1005 WP_019694074.1 aspartate carbamoyltransferase catalytic subunit -
  AO353_RS08140 (AO353_08140) - 1795632..1796903 (+) 1272 WP_054594457.1 dihydroorotase -
  AO353_RS08145 (AO353_08145) - 1796948..1797367 (-) 420 WP_019694078.1 NINE protein -
  AO353_RS08150 (AO353_08150) - 1797619..1798242 (+) 624 WP_054594458.1 C40 family peptidase -
  AO353_RS08155 (AO353_08155) pilT 1798387..1799421 (-) 1035 WP_054594459.1 type IV pilus twitching motility protein PilT Machinery gene
  AO353_RS08160 (AO353_08160) - 1799478..1800176 (+) 699 WP_054594460.1 YggS family pyridoxal phosphate-dependent enzyme -
  AO353_RS08165 (AO353_08165) proC 1800210..1801028 (+) 819 WP_054594461.1 pyrroline-5-carboxylate reductase -
  AO353_RS08170 (AO353_08170) - 1801053..1801640 (+) 588 WP_054594462.1 YggT family protein -
  AO353_RS08175 (AO353_08175) - 1801640..1801936 (+) 297 WP_054594463.1 DUF167 domain-containing protein -
  AO353_RS08180 (AO353_08180) metX 1802144..1803283 (+) 1140 WP_054594464.1 homoserine O-succinyltransferase MetX -
  AO353_RS08185 (AO353_08185) metW 1803291..1803911 (+) 621 WP_054594465.1 methionine biosynthesis protein MetW -
  AO353_RS08190 (AO353_08190) - 1803936..1804370 (+) 435 WP_054594466.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38215.73 Da        Isoelectric Point: 7.3191

>NTDB_id=157776 AO353_RS08155 WP_054594459.1 1798387..1799421(-) (pilT) [Pseudomonas fluorescens strain FW300-N2E3]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDDKQVRELIYAIMNDKQRVEFEQALETDFSFEAPGVAR
FRVNAFNQYRGPGAVFRTIPSKIQSMEDLGMGNVFRRIAEVSRGLVLVTGPTGSGKSTTQAAMIDYLNNHRHHHILTIED
PIEFLHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTNSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQSLIKKIGGGRVAAHEIMLGTSAIRNLIREDKIAQMYSSIQTGGSLGMQTLDMSL
KDLVNKGLITREHARERARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=157776 AO353_RS08155 WP_054594459.1 1798387..1799421(-) (pilT) [Pseudomonas fluorescens strain FW300-N2E3]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAAGGGGCTTCCGACTTGCACCTGTCGGCGGGGCTGCCGCCGAT
GATCCGGGTCGATGGCGATGTGCGGCGGATCAACCTGCCGGCGCTGGATGACAAGCAGGTGCGCGAGCTGATCTACGCGA
TCATGAACGACAAGCAGCGGGTGGAGTTCGAGCAGGCGCTGGAGACCGACTTTTCCTTCGAGGCGCCGGGTGTGGCGCGC
TTTCGGGTCAATGCGTTCAATCAGTACCGCGGCCCGGGCGCGGTGTTCCGGACCATCCCGTCGAAAATCCAGAGCATGGA
AGATCTGGGCATGGGGAATGTGTTTCGCCGTATTGCCGAGGTCTCGCGCGGGCTGGTGCTGGTCACCGGGCCGACAGGTT
CCGGTAAGTCGACCACCCAGGCGGCAATGATCGATTACCTGAATAACCACCGGCATCACCATATCCTCACCATCGAAGAC
CCTATCGAGTTCCTCCATGAATCACGCAAATGCCTGATCAATCAGCGTGAAGTGCACCGCGATACGCGCAGTTTCGCTAC
GGCGTTGCGTTCGGCATTGCGCGAGGACCCGGATGTGATTCTGGTAGGGGAAATGCGTGATCTGGAGACTATCCGCCTGG
CGCTGACGGCGGCCGAGACCGGGCATCTGGTCTTTGGCACCCTGCACACCAACTCGGCGGCGAAAACCATCGATCGGGTG
GTGGACGTCTTTCCGGGGGACGAGAAGTCGATGGTGCGTTCGATGCTCTCCGAGTCACTGCAGGCAGTGATTTCCCAGAG
CCTCATCAAGAAAATCGGTGGTGGCCGGGTGGCGGCTCACGAAATCATGCTGGGCACCTCGGCAATCCGTAACCTGATCC
GCGAAGACAAGATTGCACAGATGTACTCGTCGATTCAGACCGGGGGTTCGTTGGGAATGCAGACGCTGGATATGAGCTTG
AAGGATCTGGTGAACAAAGGCCTGATCACTCGCGAGCATGCGCGGGAGAGGGCGCGGTCACCGGATAATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8C5V9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

84.012

100

0.84
  pilT Pseudomonas stutzeri DSM 10701

83.721

100

0.837
  pilT Acinetobacter nosocomialis M2

75.872

100

0.759
  pilT Acinetobacter baumannii D1279779

75.872

100

0.759
  pilT Acinetobacter baumannii strain A118

75.872

100

0.759
  pilT Acinetobacter baylyi ADP1

75.291

100

0.753
  pilT Legionella pneumophila strain Lp02

72.384

100

0.724
  pilT Legionella pneumophila strain ERS1305867

72.384

100

0.724
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.656

97.965

0.663
  pilT Vibrio cholerae strain A1552

67.656

97.965

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Acinetobacter baylyi ADP1

42.486

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Vibrio cholerae strain A1552

41.159

100

0.413

Multiple sequence alignment