Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AOY20_RS07060 Genome accession   NZ_CP012808
Coordinates   1503825..1504862 (-) Length   345 a.a.
NCBI ID   WP_054581204.1    Uniprot ID   -
Organism   Acinetobacter equi strain 114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1498825..1509862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY20_RS07030 (AOY20_07030) argB 1499122..1500030 (+) 909 WP_054581198.1 acetylglutamate kinase -
  AOY20_RS07035 (AOY20_07035) - 1500227..1501075 (+) 849 WP_054581199.1 class II glutamine amidotransferase -
  AOY20_RS07040 (AOY20_07040) - 1501344..1502426 (+) 1083 WP_054581200.1 hypothetical protein -
  AOY20_RS07045 (AOY20_07045) - 1502426..1502752 (+) 327 WP_054581201.1 RnfH family protein -
  AOY20_RS07050 (AOY20_07050) - 1502800..1503198 (-) 399 WP_054581202.1 outer membrane protein assembly factor BamE -
  AOY20_RS07055 (AOY20_07055) fur 1503310..1503747 (+) 438 WP_054581203.1 ferric iron uptake transcriptional regulator -
  AOY20_RS07060 (AOY20_07060) pilT 1503825..1504862 (-) 1038 WP_054581204.1 type IV pilus twitching motility protein PilT Machinery gene
  AOY20_RS07065 (AOY20_07065) - 1504985..1505674 (+) 690 WP_054581205.1 YggS family pyridoxal phosphate-dependent enzyme -
  AOY20_RS07070 (AOY20_07070) - 1505843..1506481 (+) 639 WP_227510369.1 flagellar biosynthesis protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38767.54 Da        Isoelectric Point: 6.4337

>NTDB_id=157761 AOY20_RS07060 WP_054581204.1 1503825..1504862(-) (pilT) [Acinetobacter equi strain 114]
MNIIELLTFAIKNNASDLHLSAGMPPLIRVDGEIRRINVPHLEQENVQEFLYDTMNEKQQREFEEHLEIDFSYEIENLAR
FRVNAFHQKRGISAVFRTIPSKVLSMDELNMGQVFKNICDFPRGLVLVTGPTGSGKSTTLAAMIDYINEQRYSHILTVED
PIEFVHQSKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTVDRV
IDVFPAEEKEMVRAMLSESLQAVISQSLLKKNGGGRVAAYEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMITLDQSL
KNLVAKGLISPQTARSMAKQPETFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=157761 AOY20_RS07060 WP_054581204.1 1503825..1504862(-) (pilT) [Acinetobacter equi strain 114]
ATGAATATTATAGAACTACTAACGTTTGCAATAAAAAATAATGCCTCAGATCTACATTTATCTGCAGGAATGCCACCACT
AATAAGAGTAGATGGAGAAATTAGACGAATCAATGTACCTCATCTTGAACAAGAAAATGTACAAGAATTCTTATATGACA
CAATGAATGAAAAACAACAACGAGAATTTGAAGAACACCTAGAAATAGATTTTTCCTATGAAATTGAAAATTTAGCTCGT
TTTCGTGTAAATGCTTTTCATCAAAAACGTGGTATTAGCGCTGTATTTAGAACTATTCCAAGTAAAGTTTTAAGTATGGA
TGAACTCAATATGGGGCAAGTATTTAAAAATATCTGTGATTTTCCTCGAGGTCTTGTGTTGGTTACAGGTCCAACGGGTT
CAGGTAAGTCTACAACATTAGCTGCAATGATTGATTATATTAACGAGCAACGTTATAGCCATATTTTAACTGTTGAAGAT
CCTATTGAGTTTGTGCATCAATCGAAAAAATGTTTAGTGAATCAACGAGAAGTTCATCGAGATACGCATGGCTTTAATGA
AGCATTACGGTCAGCGTTACGTGAAGACCCAGATATTATTTTAGTGGGTGAAATGCGCGATCTTGAAACAATCCGTTTGG
CATTAACTGCTGCTGAAACTGGTCATCTCGTTTTTGGTACATTACATACAACTTCGGCTGCAAAAACAGTAGACCGAGTA
ATTGATGTATTTCCTGCAGAAGAAAAAGAAATGGTGCGAGCAATGCTTTCAGAATCATTACAGGCTGTCATTTCACAAAG
TTTACTGAAAAAAAATGGCGGTGGTCGTGTTGCTGCTTATGAAATTATGATTGGCATTCCTGCTATTCGTAATTTAATCA
GAGAAAATAAAGTTGCCCAAATGTATTCAGCCATTCAAACTGGCGCGAATCACGGTATGATCACTTTAGATCAAAGTTTA
AAAAACCTTGTAGCAAAAGGATTAATTTCACCTCAAACTGCCCGCTCTATGGCAAAACAGCCCGAAACATTTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

83.768

100

0.838
  pilT Acinetobacter baumannii strain A118

83.768

100

0.838
  pilT Acinetobacter nosocomialis M2

83.478

100

0.835
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.035

99.71

0.768
  pilT Pseudomonas stutzeri DSM 10701

75.872

99.71

0.757
  pilT Legionella pneumophila strain Lp02

71.512

99.71

0.713
  pilT Legionella pneumophila strain ERS1305867

71.512

99.71

0.713
  pilT Neisseria meningitidis 8013

65.217

100

0.652
  pilT Neisseria gonorrhoeae MS11

64.928

100

0.649
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.179

97.391

0.635
  pilT Vibrio cholerae strain A1552

65.179

97.391

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Vibrio cholerae strain A1552

42.388

97.101

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.192

99.42

0.38

Multiple sequence alignment