Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AN479_RS13290 Genome accession   NZ_CP012639
Coordinates   2847044..2848066 (+) Length   340 a.a.
NCBI ID   WP_060660247.1    Uniprot ID   -
Organism   Serratia marcescens strain RSC-14     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2842044..2853066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AN479_RS13255 (AN479_13200) - 2842383..2842817 (+) 435 WP_016930094.1 DUF29 domain-containing protein -
  AN479_RS13260 (AN479_13205) hemW 2842856..2843998 (-) 1143 WP_033644669.1 radical SAM family heme chaperone HemW -
  AN479_RS13265 (AN479_13210) - 2843991..2844584 (-) 594 WP_042785371.1 XTP/dITP diphosphatase -
  AN479_RS13270 (AN479_13215) yggU 2844602..2844892 (-) 291 WP_004948014.1 DUF167 family protein YggU -
  AN479_RS13275 (AN479_13220) - 2844889..2845443 (-) 555 WP_033644671.1 YggT family protein -
  AN479_RS13280 (AN479_13225) proC 2845475..2846296 (-) 822 WP_033644672.1 pyrroline-5-carboxylate reductase -
  AN479_RS13285 (AN479_13230) - 2846316..2847023 (-) 708 WP_033644673.1 YggS family pyridoxal phosphate-dependent enzyme -
  AN479_RS13290 (AN479_13235) pilT 2847044..2848066 (+) 1023 WP_060660247.1 type IV pilus twitching motility protein PilT Machinery gene
  AN479_RS13295 (AN479_13240) ruvX 2848063..2848485 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  AN479_RS13300 (AN479_13245) - 2848485..2849048 (-) 564 WP_004937452.1 YqgE/AlgH family protein -
  AN479_RS13305 (AN479_13250) gshB 2849194..2850144 (-) 951 WP_033651325.1 glutathione synthase -
  AN479_RS13310 (AN479_13255) rsmE 2850155..2850886 (-) 732 WP_025304244.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AN479_RS13315 (AN479_13260) endA 2850956..2851651 (-) 696 WP_060660248.1 deoxyribonuclease I -
  AN479_RS13320 (AN479_13265) - 2851753..2852265 (-) 513 WP_033651326.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36423.72 Da        Isoelectric Point: 7.2888

>NTDB_id=155925 AN479_RS13290 WP_060660247.1 2847044..2848066(+) (pilT) [Serratia marcescens strain RSC-14]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGAQALCDGLLNAQQRESLRRLGQVDLALHRPSGE
RLRANVFQQSAGISLALRRIAGQSPSLAELAAPAIVPALLRRADGLILVTGATGSGKSTTLAAMIDEINRHQQRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDTALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCAGEGMGV

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=155925 AN479_RS13290 WP_060660247.1 2847044..2848066(+) (pilT) [Serratia marcescens strain RSC-14]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGCGCAGGCCCTCTGCGATG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACAGGCCGAGCGGGGAG
CGGTTGCGCGCCAACGTTTTTCAACAAAGCGCGGGGATTTCTCTCGCGTTGCGGCGTATCGCCGGGCAATCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGCCGACGGATTGATCCTGGTCACCGGCGCCACCG
GCAGCGGTAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCAGCGGCATATTTTGACGCTGGAA
GATCCGATCGAGTTCCTGCACCGCAGCCGGCGATCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TACGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATACCGCCACCATCCGGC
TGGCGCTCACTGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTAGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCGGGCAGCCTGCAAGCGGTGATCGCGCA
AAAGCTGATGAGGCGCCCCGGCGGCGGACGAGTGGCGATCTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGTGAAGGGAAAACGCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGTGTGTTGGGGGACTGCGCGGGGGAGGGAATGGGCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.057

97.353

0.497
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Neisseria meningitidis 8013

50.151

97.353

0.488
  pilT Neisseria gonorrhoeae MS11

49.849

97.353

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.368

99.118

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.353

0.476
  pilT Legionella pneumophila strain Lp02

48.943

97.353

0.476
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.58

95.294

0.368

Multiple sequence alignment