Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CRECT_RS02300 Genome accession   NZ_CP012543
Coordinates   464356..465438 (+) Length   360 a.a.
NCBI ID   WP_004320052.1    Uniprot ID   A0A6G5QKI5
Organism   Campylobacter rectus strain ATCC 33238     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459356..470438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRECT_RS02270 (CRECT_0442) hisG 460815..461423 (-) 609 WP_004320112.1 ATP phosphoribosyltransferase -
  CRECT_RS02275 (CRECT_0443) - 461417..462046 (-) 630 WP_004320092.1 type III pantothenate kinase -
  CRECT_RS02280 (CRECT_0444) - 462033..462365 (-) 333 WP_004320056.1 hypothetical protein -
  CRECT_RS02285 (CRECT_0445) - 462362..463372 (-) 1011 WP_004320050.1 hypothetical protein -
  CRECT_RS02290 (CRECT_0446) - 463369..463935 (-) 567 WP_004320101.1 hypothetical protein -
  CRECT_RS02295 (CRECT_0447) gatC 464059..464346 (+) 288 WP_004320068.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CRECT_RS02300 (CRECT_0448) pilT 464356..465438 (+) 1083 WP_004320052.1 type IV pilus twitching motility protein PilT Machinery gene
  CRECT_RS02305 (CRECT_0449) - 465438..466025 (+) 588 WP_004320108.1 CvpA family protein -
  CRECT_RS02310 (CRECT_0450) - 466028..466501 (+) 474 WP_004320130.1 Fur family transcriptional regulator -
  CRECT_RS02315 (CRECT_0451) lysS 466501..468006 (+) 1506 WP_227932336.1 lysine--tRNA ligase -
  CRECT_RS02320 (CRECT_0452) - 468003..469247 (+) 1245 WP_004320095.1 serine hydroxymethyltransferase -
  CRECT_RS02325 (CRECT_0453) - 469247..469789 (+) 543 WP_004320117.1 DUF1882 domain-containing protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40043.78 Da        Isoelectric Point: 6.3485

>NTDB_id=155050 CRECT_RS02300 WP_004320052.1 464356..465438(+) (pilT) [Campylobacter rectus strain ATCC 33238]
MEENEKFNTSLEVLLKTVVHNKASDLHIVSRSEPQIRIDGTLRPLELGVLSGHDIQDICYALITDVQKSELEENRELDFA
IELPGVGRFRGNYYYTMNGDLAAAFRIIPTEIPSLDDLKAPNIFKEVVKREKGMILVTGPTGSGKSTTLAAMLNEINLNE
RKHVITVEDPVEFVHTNKKALFSHRNVGTDTQSYARALKYALREDPDIILVGELRDRETISTAITAAETGHLVFGTLHTN
SAIQTINRIIDSFEGGEQLQVRNMLSVSLTAVISQALLPKIGSGRLAVHEILINNNAIANLIRENKVHQIYSQMQLNQQS
TGMITQTQSLMKAIRANQITKEMAMRYSTNQQELGGLLGI

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=155050 CRECT_RS02300 WP_004320052.1 464356..465438(+) (pilT) [Campylobacter rectus strain ATCC 33238]
ATGGAAGAAAACGAGAAATTTAACACCAGCCTTGAAGTACTGTTAAAAACAGTTGTTCATAATAAAGCGAGCGACTTGCA
TATCGTATCAAGAAGCGAGCCGCAAATCAGAATAGACGGCACCTTAAGGCCGCTAGAGCTTGGAGTTTTGAGCGGACACG
ACATACAAGACATCTGCTATGCGCTCATAACCGACGTGCAAAAAAGCGAACTCGAAGAAAACAGAGAGCTTGACTTTGCG
ATAGAGCTTCCCGGCGTCGGACGCTTCAGAGGCAACTACTACTACACGATGAACGGCGATTTGGCCGCTGCGTTTCGTAT
CATCCCGACTGAGATTCCTTCGCTGGACGATCTTAAAGCGCCTAATATCTTCAAAGAAGTCGTAAAACGCGAAAAAGGAA
TGATACTGGTCACCGGACCTACGGGTAGCGGTAAATCGACTACGCTTGCGGCTATGCTAAACGAGATAAATTTAAACGAA
AGAAAGCACGTAATCACCGTAGAAGATCCGGTGGAGTTTGTTCATACGAATAAAAAAGCTCTTTTTTCCCACAGAAACGT
AGGCACGGATACGCAATCTTACGCTAGAGCTCTTAAATATGCTCTGCGCGAGGATCCCGACATTATCTTGGTCGGCGAGC
TTCGCGACAGAGAGACTATATCGACCGCGATCACGGCGGCCGAGACCGGACACTTGGTATTTGGCACTTTGCATACGAAC
TCGGCTATCCAGACTATCAACCGTATCATAGATAGCTTCGAGGGCGGCGAGCAGCTTCAGGTGCGAAATATGCTCTCCGT
TTCGCTAACGGCGGTCATATCTCAAGCTCTACTTCCTAAAATCGGCAGCGGACGACTTGCCGTGCATGAAATTTTAATAA
ACAATAATGCCATAGCAAACCTTATCCGTGAAAATAAAGTGCATCAAATTTACTCTCAGATGCAGTTAAATCAACAATCT
ACGGGTATGATAACGCAGACCCAGTCTTTAATGAAAGCCATTAGGGCAAATCAGATAACCAAAGAGATGGCGATGAGGTA
CTCTACAAACCAACAAGAGCTCGGCGGACTGCTAGGTATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G5QKI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

52.959

93.889

0.497
  pilT Legionella pneumophila strain ERS1305867

52.959

93.889

0.497
  pilT Neisseria gonorrhoeae MS11

52.339

95

0.497
  pilT Neisseria meningitidis 8013

52.047

95

0.494
  pilT Acinetobacter baumannii strain A118

51.765

94.444

0.489
  pilT Acinetobacter nosocomialis M2

51.765

94.444

0.489
  pilT Acinetobacter baumannii D1279779

51.765

94.444

0.489
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.489

89.722

0.489
  pilT Vibrio cholerae strain A1552

54.489

89.722

0.489
  pilT Acinetobacter baylyi ADP1

52.366

88.056

0.461
  pilT Pseudomonas stutzeri DSM 10701

48.529

94.444

0.458
  pilT Pseudomonas aeruginosa PAK

49.547

91.944

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

95.833

0.397
  pilU Pseudomonas stutzeri DSM 10701

39.306

96.111

0.378
  pilU Vibrio cholerae strain A1552

39.003

94.722

0.369
  pilU Acinetobacter baylyi ADP1

38.484

95.278

0.367

Multiple sequence alignment