Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BBEV_RS04915 Genome accession   NZ_CP012502
Coordinates   1087070..1088101 (+) Length   343 a.a.
NCBI ID   WP_069364454.1    Uniprot ID   A0A1D7QTK9
Organism   Salisediminibacterium beveridgei strain MLTeJB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1082070..1093101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBEV_RS04900 (BBEV_0979) - 1083216..1083851 (+) 636 WP_069364451.1 hypothetical protein -
  BBEV_RS04905 (BBEV_0980) - 1083881..1085344 (+) 1464 WP_069364452.1 VanW family protein -
  BBEV_RS04910 (BBEV_0981) - 1085369..1087033 (+) 1665 WP_069364453.1 GspE/PulE family protein -
  BBEV_RS04915 (BBEV_0982) pilT 1087070..1088101 (+) 1032 WP_069364454.1 type IV pilus twitching motility protein PilT Machinery gene
  BBEV_RS04920 (BBEV_0983) - 1088131..1089339 (+) 1209 WP_069364455.1 type II secretion system F family protein -
  BBEV_RS17105 (BBEV_0984) - 1089662..1090135 (+) 474 WP_232318262.1 type II secretion system protein -
  BBEV_RS17795 (BBEV_0985) - 1090247..1090735 (+) 489 WP_084007224.1 Tfp pilus assembly protein FimT/FimU -
  BBEV_RS04935 (BBEV_0986) - 1090940..1091701 (+) 762 WP_069366599.1 A24 family peptidase -
  BBEV_RS04940 (BBEV_0987) pilM 1092028..1093011 (+) 984 WP_069364456.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37985.80 Da        Isoelectric Point: 6.3143

>NTDB_id=154745 BBEV_RS04915 WP_069364454.1 1087070..1088101(+) (pilT) [Salisediminibacterium beveridgei strain MLTeJB]
MKEMIEELLKKAYTVGASDLHLTVGVPPILRRNGELEKVGDENLKPADTEGMARAIVPEELWMNFEKKGELDFSYGLPGV
SRFGINTYFQRSCVSLAIRVIPSEVPTIEELKMPKLLEAIAARPQGLVLVTGPTGSGKSTTLAAMIDYMNTQMSRHIITL
EDPIEYLHKHNQSIIDQREIGFDTQNFANGLRASLRQDPDVILVGEMRDLETISTAITAAETGHLVLGTLHTTDAPSTID
RIIDVFPPSQQDQIRIQMASVLTSVISQRLLRTADGKGRVAATELLMNNPAVKNLIRSGKIHQIPSTMQTGKAEGMHTME
MSVRSLLKNRKIREETAAYFLPE

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=154745 BBEV_RS04915 WP_069364454.1 1087070..1088101(+) (pilT) [Salisediminibacterium beveridgei strain MLTeJB]
ATGAAAGAAATGATTGAAGAACTTCTGAAAAAAGCCTACACCGTCGGTGCGTCGGATTTACATCTCACCGTCGGTGTCCC
GCCGATCTTAAGACGGAACGGGGAGCTGGAGAAAGTGGGTGATGAGAACCTCAAGCCTGCGGACACGGAAGGTATGGCGC
GAGCAATCGTACCCGAAGAACTCTGGATGAACTTCGAAAAGAAGGGTGAACTGGATTTCTCTTACGGGTTACCCGGTGTA
TCCCGGTTTGGAATCAATACGTACTTTCAGCGCTCCTGTGTGAGCCTTGCGATCCGGGTCATTCCATCGGAGGTCCCGAC
GATTGAGGAACTGAAAATGCCGAAACTGCTTGAAGCCATTGCAGCAAGGCCCCAGGGACTCGTTCTCGTCACCGGCCCGA
CAGGCTCCGGGAAATCGACGACCCTGGCGGCGATGATCGACTATATGAATACACAGATGTCACGCCATATCATCACCCTG
GAAGACCCGATCGAATATTTGCATAAACATAATCAGTCAATCATTGACCAGCGGGAAATCGGCTTTGACACACAGAACTT
CGCCAACGGGCTTCGGGCGTCACTGCGTCAGGACCCGGACGTGATTCTCGTCGGTGAGATGCGTGACCTTGAAACGATCT
CGACTGCGATCACCGCAGCGGAAACCGGTCACCTGGTGCTCGGCACCTTGCACACGACGGATGCCCCGTCCACCATTGAC
CGGATCATCGACGTCTTCCCGCCTTCCCAGCAGGACCAGATCCGCATCCAGATGGCATCGGTTTTGACATCAGTCATCTC
GCAGCGGCTGTTGCGGACGGCGGACGGCAAAGGCCGGGTCGCGGCGACTGAACTGTTAATGAACAACCCAGCGGTGAAGA
ACCTGATCCGAAGCGGGAAGATTCACCAGATTCCGTCGACGATGCAGACCGGGAAAGCGGAAGGCATGCACACCATGGAA
ATGTCGGTCAGATCACTGTTGAAAAACAGAAAGATCAGAGAAGAAACGGCGGCGTATTTTTTACCGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D7QTK9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.354

95.627

0.51
  pilT Neisseria meningitidis 8013

49.85

97.085

0.484
  pilT Neisseria gonorrhoeae MS11

49.55

97.085

0.481
  pilT Vibrio cholerae strain A1552

50.774

94.169

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.774

94.169

0.478
  pilT Acinetobacter baylyi ADP1

48.949

97.085

0.475
  pilT Acinetobacter baumannii strain A118

48.348

97.085

0.469
  pilT Acinetobacter nosocomialis M2

48.348

97.085

0.469
  pilT Acinetobacter baumannii D1279779

48.348

97.085

0.469
  pilT Pseudomonas aeruginosa PAK

47.748

97.085

0.464
  pilT Pseudomonas stutzeri DSM 10701

47.147

97.085

0.458
  pilT Legionella pneumophila strain Lp02

45.345

97.085

0.44
  pilT Legionella pneumophila strain ERS1305867

45.345

97.085

0.44
  pilU Vibrio cholerae strain A1552

40.896

97.668

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.541

97.085

0.394

Multiple sequence alignment