Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MOTHE_RS07520 Genome accession   NZ_CP012369
Coordinates   1490627..1491646 (-) Length   339 a.a.
NCBI ID   WP_011393062.1    Uniprot ID   -
Organism   Moorella thermoacetica strain DSM 521     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1485627..1496646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOTHE_RS07490 (MOTHE_c15390) - 1486106..1486606 (-) 501 WP_011393056.1 ComGF family competence protein -
  MOTHE_RS07495 (MOTHE_c15400) - 1486603..1487001 (-) 399 WP_011393057.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MOTHE_RS07500 (MOTHE_c15410) - 1486998..1487519 (-) 522 WP_071522541.1 type II secretion system protein -
  MOTHE_RS07505 (MOTHE_c15420) - 1487551..1488294 (-) 744 WP_011393059.1 A24 family peptidase -
  MOTHE_RS07510 (MOTHE_c15430) - 1488463..1488978 (-) 516 WP_011393060.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MOTHE_RS07515 (MOTHE_c15440) - 1489223..1490431 (-) 1209 WP_011393061.1 type II secretion system F family protein -
  MOTHE_RS07520 (MOTHE_c15450) pilT 1490627..1491646 (-) 1020 WP_011393062.1 type IV pilus twitching motility protein PilT Machinery gene
  MOTHE_RS07525 (MOTHE_c15460) pilF 1491660..1493321 (-) 1662 WP_011393063.1 GspE/PulE family protein Machinery gene
  MOTHE_RS07530 (MOTHE_c15470) aroB 1493327..1494421 (-) 1095 WP_011393064.1 3-dehydroquinate synthase -
  MOTHE_RS07535 (MOTHE_c15480) - 1494400..1494921 (-) 522 WP_011393065.1 shikimate kinase -
  MOTHE_RS07540 (MOTHE_c15490) aroC 1494974..1496125 (-) 1152 WP_053094862.1 chorismate synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 37047.67 Da        Isoelectric Point: 6.7282

>NTDB_id=153854 MOTHE_RS07520 WP_011393062.1 1490627..1491646(-) (pilT) [Moorella thermoacetica strain DSM 521]
MLDIETILVAAAAAGASDVHISVGLPPVFRVHGELQVQRQWDPLDLEMTARLVRPIVGDKWEVFQEQGEIDLAYSLPGVS
RFRVNVFHQRGSVGAAIRLIPREIPSLESLGLPPVVAELAGRQHGLILVTGPTGSGKSTTLAAMVDKINRERSCHIITLE
DPIEYLHQHRRSIVNQREVGSDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAVQSVDR
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKTHQIVSTMQTGARYGMQTMEM
ALRQLITRGVIFEEGLNIG

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=153854 MOTHE_RS07520 WP_011393062.1 1490627..1491646(-) (pilT) [Moorella thermoacetica strain DSM 521]
ATGCTCGATATCGAAACAATCCTGGTAGCGGCTGCCGCCGCCGGTGCCTCCGACGTCCATATCTCTGTTGGGCTACCGCC
GGTTTTCCGGGTGCACGGCGAACTCCAGGTCCAGCGCCAGTGGGACCCCCTGGATTTGGAGATGACAGCCCGCCTGGTAC
GGCCTATAGTGGGCGATAAGTGGGAGGTATTTCAGGAGCAAGGTGAAATCGATCTGGCTTACTCCCTGCCCGGTGTAAGC
CGTTTCCGGGTCAATGTCTTTCACCAGCGGGGGAGCGTCGGGGCGGCCATCCGCCTTATCCCCAGGGAGATACCCAGCCT
GGAGTCCCTGGGTTTACCGCCGGTGGTGGCCGAACTGGCGGGCAGACAGCACGGCCTGATCCTGGTGACGGGGCCGACGG
GCAGCGGTAAATCCACCACCCTGGCGGCCATGGTGGATAAAATCAACCGGGAGCGGAGCTGTCACATCATCACCCTGGAA
GACCCCATTGAGTACTTGCACCAGCACCGCCGCAGCATAGTCAACCAGCGGGAAGTGGGTTCTGATACCAGGTCCTTTGC
CAGCGCCCTGCGGGCCGCCCTGCGTCAGGACCCCGACGTTATCCTGGTCGGGGAGATGCGCGACCTGGAAACCATCGCCA
CGGCCATTACGGCTGCCGAAACAGGTCACCTGGTCCTGGCGACTTTGCACACCAGCAGTGCCGTCCAGAGTGTGGATCGG
ATCATTGACGTTTTCCCGCCCCACCAGCAGGGGCAGGTACGGATCCAGCTTGCCGACACCCTGGAAGGGGTGATCACCCA
GCAGCTTTTACCGCGGGCCGACAGGAAGGGCCGGGTGGCGGCTGTAGAAGTGTTGATAGCCACCCCGGCAGTGAAGAATC
TCATCCGCGAGGGTAAAACCCATCAAATCGTTTCCACTATGCAGACCGGAGCCCGCTACGGGATGCAGACCATGGAGATG
GCGCTGCGGCAACTGATCACCCGGGGAGTAATTTTTGAGGAAGGTTTAAATATTGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.083

99.41

0.558
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.807

97.935

0.507
  pilT Vibrio cholerae strain A1552

51.807

97.935

0.507
  pilT Acinetobacter nosocomialis M2

51.205

97.935

0.501
  pilT Acinetobacter baumannii strain A118

51.205

97.935

0.501
  pilT Acinetobacter baumannii D1279779

51.205

97.935

0.501
  pilT Acinetobacter baylyi ADP1

50.904

97.935

0.499
  pilT Pseudomonas stutzeri DSM 10701

50.602

97.935

0.496
  pilT Pseudomonas aeruginosa PAK

49.851

98.82

0.493
  pilT Neisseria meningitidis 8013

49.401

98.525

0.487
  pilT Neisseria gonorrhoeae MS11

48.955

98.82

0.484
  pilT Legionella pneumophila strain Lp02

49.245

97.64

0.481
  pilT Legionella pneumophila strain ERS1305867

49.245

97.64

0.481
  pilU Pseudomonas stutzeri DSM 10701

44.97

99.705

0.448
  pilU Vibrio cholerae strain A1552

45.312

94.395

0.428
  pilU Acinetobacter baylyi ADP1

43.252

96.165

0.416

Multiple sequence alignment