Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CUREO_RS06960 Genome accession   NZ_CP012195
Coordinates   1375644..1376726 (+) Length   360 a.a.
NCBI ID   WP_050335617.1    Uniprot ID   A0AAU8U591
Organism   Campylobacter ureolyticus RIGS 9880     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1370644..1381726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUREO_RS06925 (CUREO_1391) - 1371216..1372118 (+) 903 WP_050335603.1 LysR substrate-binding domain-containing protein -
  CUREO_RS06930 (CUREO_1392) hisG 1372104..1372712 (-) 609 WP_050335607.1 ATP phosphoribosyltransferase -
  CUREO_RS06935 (CUREO_1393) - 1372709..1373341 (-) 633 WP_050335608.1 type III pantothenate kinase -
  CUREO_RS06940 (CUREO_1394) - 1373323..1373649 (-) 327 WP_235436856.1 hypothetical protein -
  CUREO_RS06945 (CUREO_1395) - 1373654..1374661 (-) 1008 WP_050335613.1 hypothetical protein -
  CUREO_RS06950 (CUREO_1396) - 1374648..1375226 (-) 579 WP_050335614.1 hypothetical protein -
  CUREO_RS06955 (CUREO_1397) gatC 1375350..1375631 (+) 282 WP_050335615.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CUREO_RS06960 (CUREO_1398) pilT 1375644..1376726 (+) 1083 WP_050335617.1 type IV pilus twitching motility protein PilT Machinery gene
  CUREO_RS06965 (CUREO_1399) - 1376727..1377371 (+) 645 WP_050335619.1 CvpA family protein -
  CUREO_RS06970 (CUREO_1400) - 1377340..1377822 (+) 483 WP_034965418.1 Fur family transcriptional regulator -
  CUREO_RS06975 (CUREO_1401) lysS 1377826..1379337 (+) 1512 WP_050335621.1 lysine--tRNA ligase -
  CUREO_RS06980 (CUREO_1402) - 1379340..1380584 (+) 1245 WP_024962301.1 serine hydroxymethyltransferase -
  CUREO_RS06985 (CUREO_1403) - 1380593..1381477 (+) 885 WP_050335622.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40163.02 Da        Isoelectric Point: 6.5057

>NTDB_id=152136 CUREO_RS06960 WP_050335617.1 1375644..1376726(+) (pilT) [Campylobacter ureolyticus RIGS 9880]
MEKSNIKTDIQTLLKVQINNDASDLHIVSRSAPQIRIDGKLVPLDMPNLTGTDIEHLCYAIITDAQKSELEEKKELDFAM
EIPNIGRFRGNYYYTMNGDLAAAFRKIPVDIPSLDDLRAPNIFKELVKREKGMILVTGPTGSGKSTTLAAMLNEINLTER
KHIITVEDPVEFVHENKKCLFSHRNIGTDTHSYANALKYALREDPDIILIGEMRDRETISIAITAAETGHLVFATLHTNS
AMQTISRIVDSFDGSEQTQIRNMLSVSLSAIISQSLLPKIGKGRLAVHEILVNNHAIANLIREDKTQQIYSQMQLNQQET
GMVTQTQALLQALNKRLISKESALAYTTNRQELVKALGGE

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=152136 CUREO_RS06960 WP_050335617.1 1375644..1376726(+) (pilT) [Campylobacter ureolyticus RIGS 9880]
ATGGAAAAAAGCAATATAAAAACTGATATTCAAACCCTTTTAAAGGTACAAATAAATAACGATGCAAGTGACCTTCATAT
AGTAAGCAGAAGTGCCCCACAAATTAGAATCGATGGAAAATTAGTTCCTCTTGATATGCCAAATTTAACAGGAACAGACA
TTGAACATCTTTGTTATGCAATTATTACAGATGCACAAAAAAGTGAACTCGAAGAGAAAAAAGAGCTTGACTTTGCTATG
GAAATTCCAAACATAGGAAGGTTTAGAGGAAATTATTATTATACTATGAATGGTGATTTAGCAGCTGCATTTAGAAAAAT
TCCTGTTGATATTCCTTCTCTTGATGATTTAAGAGCTCCAAATATTTTTAAAGAGCTAGTTAAAAGAGAAAAAGGGATGA
TTTTAGTAACTGGTCCTACAGGAAGTGGTAAATCAACTACTTTGGCTGCAATGTTAAATGAGATAAATTTAACTGAAAGA
AAGCATATTATAACAGTTGAAGACCCAGTTGAGTTTGTTCATGAAAACAAAAAATGTCTTTTTTCTCATAGAAATATAGG
AACCGATACTCACTCTTATGCAAATGCTTTAAAATATGCTCTTCGTGAGGACCCTGATATTATACTAATTGGTGAGATGA
GAGATAGAGAAACTATTTCAATTGCCATTACAGCAGCTGAAACAGGACACTTAGTTTTTGCTACACTTCACACAAACTCA
GCTATGCAAACAATCAGCCGTATAGTTGATAGTTTCGATGGAAGTGAACAAACACAAATAAGAAATATGCTATCAGTTTC
ATTAAGTGCAATAATTTCACAATCCCTACTTCCAAAAATTGGAAAAGGGCGACTTGCAGTTCATGAAATTTTGGTAAATA
ATCACGCTATTGCCAACCTAATAAGAGAAGACAAAACTCAACAAATTTATTCTCAAATGCAGCTAAATCAACAAGAAACA
GGTATGGTAACTCAAACTCAAGCACTATTACAAGCACTCAATAAAAGACTTATTTCAAAAGAAAGTGCTCTTGCTTACAC
TACAAATAGACAAGAGTTAGTTAAGGCTTTAGGGGGTGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

51.754

95

0.492
  pilT Legionella pneumophila strain ERS1305867

51.754

95

0.492
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.853

92.5

0.489
  pilT Vibrio cholerae strain A1552

52.853

92.5

0.489
  pilT Acinetobacter baylyi ADP1

50.742

93.611

0.475
  pilT Acinetobacter baumannii D1279779

50.746

93.056

0.472
  pilT Acinetobacter baumannii strain A118

50.746

93.056

0.472
  pilT Acinetobacter nosocomialis M2

50.746

93.056

0.472
  pilT Neisseria gonorrhoeae MS11

48.696

95.833

0.467
  pilT Pseudomonas stutzeri DSM 10701

50.149

93.056

0.467
  pilT Neisseria meningitidis 8013

48.406

95.833

0.464
  pilT Pseudomonas aeruginosa PAK

49.552

93.056

0.461
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.954

96.667

0.406
  pilU Acinetobacter baylyi ADP1

40.294

94.444

0.381
  pilU Pseudomonas stutzeri DSM 10701

38.202

98.889

0.378
  pilU Vibrio cholerae strain A1552

41.463

91.111

0.378

Multiple sequence alignment