Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   AEA01_RS05230 Genome accession   NZ_CP012146
Coordinates   1252699..1253139 (+) Length   146 a.a.
NCBI ID   WP_011038214.1    Uniprot ID   Q8P671
Organism   Xanthomonas campestris pv. campestris strain ICMP 4013     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1247699..1258139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AEA01_RS05210 (AEA01_05225) - 1248028..1249395 (+) 1368 WP_011035783.1 IS5-like element IS1478 family transposase -
  AEA01_RS05215 (AEA01_05230) coaE 1249609..1250232 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  AEA01_RS05220 (AEA01_05235) - 1250246..1251109 (-) 864 WP_011038216.1 A24 family peptidase -
  AEA01_RS05225 (AEA01_05240) pilC 1251116..1252372 (-) 1257 WP_011038215.1 type II secretion system F family protein Machinery gene
  AEA01_RS05230 (AEA01_05245) pilE 1252699..1253139 (+) 441 WP_011038214.1 pilin Machinery gene
  AEA01_RS05235 (AEA01_05250) - 1253219..1253653 (+) 435 WP_011038213.1 pilin -
  AEA01_RS05240 (AEA01_05255) pilB 1253718..1255451 (+) 1734 WP_011038212.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AEA01_RS05245 (AEA01_05260) pilR 1255868..1257262 (-) 1395 WP_012437607.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14797.93 Da        Isoelectric Point: 9.0090

>NTDB_id=151804 AEA01_RS05230 WP_011038214.1 1252699..1253139(+) (pilE) [Xanthomonas campestris pv. campestris strain ICMP 4013]
MKKQNGFTLIELMIVVAIIAILAAIALPAYQDYLARSQVSEGLSLASGAKTAVAETYANTGAFPATNAAAGLEAAANIKG
KYVTSVTVGAGGIITAAFNTANAKLSGKNLVLTPTDNNGSISWGCTNGTTIDQKYLPTSCRTAAAP

Nucleotide


Download         Length: 441 bp        

>NTDB_id=151804 AEA01_RS05230 WP_011038214.1 1252699..1253139(+) (pilE) [Xanthomonas campestris pv. campestris strain ICMP 4013]
ATGAAAAAGCAAAATGGTTTTACACTGATCGAACTCATGATCGTCGTTGCGATCATCGCTATTCTGGCCGCTATCGCTTT
GCCGGCGTACCAGGATTACCTCGCTCGTTCGCAGGTTTCGGAAGGCTTGTCTTTGGCGTCGGGTGCAAAGACAGCTGTCG
CTGAAACTTATGCTAACACCGGTGCCTTCCCGGCGACCAATGCCGCCGCTGGCCTTGAGGCTGCTGCGAACATCAAGGGT
AAGTACGTTACGTCTGTGACGGTGGGGGCGGGTGGGATCATCACCGCGGCATTCAATACTGCTAATGCAAAGTTGAGTGG
CAAGAATCTGGTCCTGACCCCGACTGACAATAACGGTTCGATCAGCTGGGGGTGCACCAACGGCACCACGATTGATCAGA
AGTATCTGCCGACCTCTTGCCGCACTGCGGCGGCTCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P671

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

46.875

100

0.514
  pilE Neisseria gonorrhoeae strain FA1090

46.541

100

0.507
  pilA2 Legionella pneumophila str. Paris

52.857

95.89

0.507
  pilA2 Legionella pneumophila strain ERS1305867

52.143

95.89

0.5
  pilA/pilA1 Eikenella corrodens VA1

46.452

100

0.493
  comP Acinetobacter baylyi ADP1

48

100

0.493
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.5

100

0.473
  pilA Ralstonia pseudosolanacearum GMI1000

40.244

100

0.452
  pilA Acinetobacter baumannii strain A118

41.333

100

0.425
  pilA Pseudomonas aeruginosa PAK

40.789

100

0.425
  pilA Vibrio campbellii strain DS40M4

40.268

100

0.411
  pilA Vibrio cholerae C6706

38.411

100

0.397
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.411

100

0.397
  pilA Vibrio cholerae strain A1552

38.411

100

0.397
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.288

95.205

0.384
  pilA Vibrio parahaemolyticus RIMD 2210633

42.4

85.616

0.363

Multiple sequence alignment