Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A176_RS05450 Genome accession   NZ_CP012109
Coordinates   1321273..1322394 (+) Length   373 a.a.
NCBI ID   WP_002638890.1    Uniprot ID   -
Organism   Myxococcus hansupus strain contaminant ex DSM 436     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1316273..1327394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A176_RS05430 (A176_001118) - 1316552..1317595 (-) 1044 WP_002638886.1 diguanylate cyclase -
  A176_RS05435 (A176_001119) trmB 1317703..1318323 (-) 621 WP_002638887.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  A176_RS05440 (A176_001120) - 1318399..1319325 (+) 927 WP_002638888.1 bifunctional riboflavin kinase/FAD synthetase -
  A176_RS05445 (A176_001121) pilB 1319530..1321230 (+) 1701 WP_002638889.1 type IV-A pilus assembly ATPase PilB -
  A176_RS05450 (A176_001122) pilT 1321273..1322394 (+) 1122 WP_002638890.1 type IV pilus twitching motility protein PilT Machinery gene
  A176_RS05455 (A176_001123) - 1322452..1323705 (+) 1254 WP_002638891.1 type II secretion system F family protein -
  A176_RS05460 (A176_001124) - 1323716..1325416 (+) 1701 WP_002638892.1 two-component system sensor histidine kinase NtrB -
  A176_RS05465 (A176_001125) - 1325527..1326909 (+) 1383 WP_082282687.1 sigma-54-dependent transcriptional regulator -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 40882.22 Da        Isoelectric Point: 8.2933

>NTDB_id=151417 A176_RS05450 WP_002638890.1 1321273..1322394(+) (pilT) [Myxococcus hansupus strain contaminant ex DSM 436]
MANLHQLLKAMVEKGASDLHVTTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAAAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDIVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQMSFVLEGVMSQALVAKAGSPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKFGMQTFN
QALASLLLRRIISQDEAFGRSSDAEELRNILATGGGNLPGMPQQRPGGGAGGR

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=151417 A176_RS05450 WP_002638890.1 1321273..1322394(+) (pilT) [Myxococcus hansupus strain contaminant ex DSM 436]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACGTCACCACTGGCTCACCGCC
GCAGCTTCGCGTCGACGGCGAGCTCGTTCCCTTGAAGACGGCGCCGCTGACCCCGGTGGAGACGAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAAGAGGAGAACGAGCTCGACTTGTCCTTCGGCGTGAAGGGGCTGTCC
CGCTTCCGCGCCAACATCTTCATGCAGCGCGGCGCCGTCGCGGCGGCGTTCCGGACCATTCCCTTCAAGATCCTGACCTT
CCAGGAGCTGGGCCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGTGGGCTCATCCTGGTGACGGGGCCCACGG
GCTCGGGCAAGTCGACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTGAACCAGCGCGAGGTGGGGGCGGACACGCGGAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACATCGTGCTCGTAGGTGAGCTCCGCGACCTGGAGACGATTGAAG
CGGCGCTGACCATCGCGGAGACGGGTCACATCTGCTACGCGACGCTCCACACGAACAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTCCGCGCGCAGATGTCCTTCGTGTTGGAGGGCGTGATGAGCCA
GGCGCTGGTGGCGAAGGCGGGCAGCCCCGGCCGCGTGCTGGCGCTGGAGGTGATGGTGCCGAACCCCGCCATCCGGAACC
TCATCCGTGAGGACAAGGTCCACCAGATCTACTCGTCCATGCAGGTCGGCCAGGCCAAGTTCGGCATGCAGACCTTCAAC
CAGGCCCTGGCGTCGCTGCTGCTGCGGCGGATCATCTCCCAGGACGAGGCCTTCGGCCGTTCTAGCGACGCCGAGGAGTT
GCGCAACATCCTGGCCACGGGCGGTGGGAACCTGCCCGGGATGCCACAGCAGCGACCAGGCGGGGGAGCGGGTGGTCGTT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

53.731

89.812

0.483
  pilT Legionella pneumophila strain Lp02

53.731

89.812

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.162

95.979

0.472
  pilT Pseudomonas stutzeri DSM 10701

52.537

89.812

0.472
  pilT Pseudomonas aeruginosa PAK

52.537

89.812

0.472
  pilT Acinetobacter baylyi ADP1

52.568

88.74

0.466
  pilT Acinetobacter baumannii D1279779

52.568

88.74

0.466
  pilT Acinetobacter baumannii strain A118

52.568

88.74

0.466
  pilT Acinetobacter nosocomialis M2

52.568

88.74

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.204

89.544

0.432
  pilT Vibrio cholerae strain A1552

48.204

89.544

0.432
  pilU Vibrio cholerae strain A1552

42.857

95.71

0.41
  pilT Neisseria meningitidis 8013

44.575

91.421

0.408
  pilT Neisseria gonorrhoeae MS11

44.575

91.421

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.171

94.102

0.378
  pilU Acinetobacter baylyi ADP1

39.193

93.029

0.365

Multiple sequence alignment