Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACG10_RS20115 Genome accession   NZ_CP011835
Coordinates   4299400..4300434 (-) Length   344 a.a.
NCBI ID   WP_089169563.1    Uniprot ID   -
Organism   Azotobacter chroococcum strain B3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4294400..4305434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG10_RS20090 (ACG10_19700) pyrR 4294853..4295362 (+) 510 WP_039806751.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACG10_RS20095 (ACG10_19705) - 4295381..4296397 (+) 1017 WP_089169561.1 aspartate carbamoyltransferase catalytic subunit -
  ACG10_RS20100 (ACG10_19710) - 4296397..4297668 (+) 1272 WP_089169562.1 dihydroorotase -
  ACG10_RS20105 (ACG10_19715) - 4297736..4298137 (-) 402 WP_039806755.1 NINE protein -
  ACG10_RS20110 (ACG10_19720) pilU 4298219..4299364 (-) 1146 WP_039806759.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG10_RS20115 (ACG10_19725) pilT 4299400..4300434 (-) 1035 WP_089169563.1 type IV pilus twitching motility protein PilT Machinery gene
  ACG10_RS20120 (ACG10_19730) - 4300506..4301198 (+) 693 WP_089169564.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACG10_RS20125 (ACG10_19735) proC 4301216..4302034 (+) 819 WP_089169565.1 pyrroline-5-carboxylate reductase -
  ACG10_RS20130 (ACG10_19740) - 4302047..4302640 (+) 594 WP_039806769.1 YggT family protein -
  ACG10_RS20135 (ACG10_19745) - 4302640..4302939 (+) 300 WP_089169566.1 DUF167 domain-containing protein -
  ACG10_RS20140 (ACG10_19750) - 4303094..4304233 (+) 1140 WP_089169962.1 homoserine O-acetyltransferase -
  ACG10_RS20145 (ACG10_19755) metW 4304241..4304834 (+) 594 WP_089169567.1 methionine biosynthesis protein MetW -
  ACG10_RS20150 (ACG10_19760) - 4304906..4305325 (+) 420 WP_089169568.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38027.82 Da        Isoelectric Point: 6.8890

>NTDB_id=148933 ACG10_RS20115 WP_089169563.1 4299400..4300434(-) (pilT) [Azotobacter chroococcum strain B3]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSRVLSMDDLGMGEVFKKITEVPRGLVLVTGPTGSGKSTTLAAMLDYLNANKHHHILTIED
PIEFVHESKKCLINQREVHRDTLGFAEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSIGMQTLDMCL
KNLLSRGAITRENAREKAKIPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=148933 ACG10_RS20115 WP_089169563.1 4299400..4300434(-) (pilT) [Azotobacter chroococcum strain B3]
ATGGACATTACCGAGCTGCTCGCCTTCAGCGCCAAGCAGGGCGCGTCGGACCTGCACCTCTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGCCGGATCAACCTGCCGCCGATGGATCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCGGGCGTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGGGCCGGCGCGGTGTTCCGGACCATTCCGTCGCGGGTGCTGAGCATGGA
CGATCTGGGGATGGGCGAGGTGTTCAAGAAGATCACCGAGGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTATCTGAACGCCAACAAGCACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTCCACGAATCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCTGGGCTTCGCCGA
GGCGCTGCGTTCGGCGCTGCGCGAGGACCCGGACGTCATCCTGGTTGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCGCGGAGGAGAAGTCGATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGGTCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGCTCGATCGGCATGCAGACCCTGGACATGTGCCTG
AAGAACCTGCTGTCCAGGGGCGCGATCACCCGCGAGAACGCCCGCGAGAAGGCGAAGATTCCCGACAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

92.151

100

0.922
  pilT Pseudomonas aeruginosa PAK

91.57

100

0.916
  pilT Acinetobacter nosocomialis M2

83.14

100

0.831
  pilT Acinetobacter baumannii D1279779

82.849

100

0.828
  pilT Acinetobacter baumannii strain A118

82.849

100

0.828
  pilT Acinetobacter baylyi ADP1

81.105

100

0.811
  pilT Legionella pneumophila strain Lp02

73.669

98.256

0.724
  pilT Legionella pneumophila strain ERS1305867

73.669

98.256

0.724
  pilT Neisseria meningitidis 8013

69.118

98.837

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.824

98.837

0.68
  pilT Vibrio cholerae strain A1552

68.824

98.837

0.68
  pilT Neisseria gonorrhoeae MS11

68.824

98.837

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Vibrio cholerae strain A1552

39.528

98.547

0.39

Multiple sequence alignment