Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FORC16_RS12405 Genome accession   NZ_CP011775
Coordinates   2736077..2737183 (-) Length   368 a.a.
NCBI ID   WP_011079463.1    Uniprot ID   A0A3Q0L3V4
Organism   Vibrio vulnificus strain FORC_016     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2731077..2742183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC16_RS12375 (FORC16_2454) - 2731809..2732315 (+) 507 WP_060530725.1 SprT family zinc-dependent metalloprotease -
  FORC16_RS12380 (FORC16_2455) - 2732416..2733111 (+) 696 WP_060530722.1 endonuclease -
  FORC16_RS12385 (FORC16_2456) rsmE 2733223..2733954 (+) 732 WP_060530721.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FORC16_RS12390 (FORC16_2457) gshB 2733969..2734922 (+) 954 WP_013571098.1 glutathione synthase -
  FORC16_RS12395 (FORC16_2458) - 2734977..2735540 (+) 564 WP_011079465.1 YqgE/AlgH family protein -
  FORC16_RS12400 (FORC16_2459) ruvX 2735562..2735984 (+) 423 WP_060530718.1 Holliday junction resolvase RuvX -
  FORC16_RS12405 (FORC16_2460) pilU 2736077..2737183 (-) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FORC16_RS12410 (FORC16_2461) pilT 2737202..2738239 (-) 1038 WP_060530716.1 type IV pilus twitching motility protein PilT Machinery gene
  FORC16_RS12415 (FORC16_2462) - 2738264..2738974 (+) 711 WP_060530715.1 YggS family pyridoxal phosphate-dependent enzyme -
  FORC16_RS12420 (FORC16_2463) proC 2739009..2739827 (+) 819 WP_060530714.1 pyrroline-5-carboxylate reductase -
  FORC16_RS12425 (FORC16_2464) - 2739869..2740426 (+) 558 WP_013571095.1 YggT family protein -
  FORC16_RS12430 (FORC16_2465) yggU 2740426..2740716 (+) 291 WP_060530713.1 DUF167 family protein YggU -
  FORC16_RS12435 (FORC16_2466) - 2740759..2741190 (+) 432 WP_011151200.1 DUF4426 domain-containing protein -
  FORC16_RS12440 (FORC16_2467) - 2741201..2741803 (+) 603 WP_011079456.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41245.35 Da        Isoelectric Point: 6.7296

>NTDB_id=148634 FORC16_RS12405 WP_011079463.1 2736077..2737183(-) (pilU) [Vibrio vulnificus strain FORC_016]
MDLNKILEGMITLKASDLYITVGAPVLFRVDGELRPQGEKLSQIEVAQLLDGAMDEERRADFRKSRESNFAIVRDSGRFR
VSAFYQRELPGAVIRRIETNIPTFEELKLPHVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEVLLNSPRVSDLIRRGDLHELKMTMAKSKEVGMQTFDQSLY
TLVVEGKISEEDAMHSADSANDLRLMLKTQRGDISSPGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=148634 FORC16_RS12405 WP_011079463.1 2736077..2737183(-) (pilU) [Vibrio vulnificus strain FORC_016]
ATGGATTTAAACAAGATTTTAGAAGGGATGATCACGCTTAAAGCGTCGGATCTTTATATCACGGTTGGTGCCCCCGTGCT
GTTTCGTGTCGATGGTGAGCTGCGACCGCAAGGCGAAAAATTATCGCAAATTGAAGTCGCTCAACTGCTGGATGGGGCGA
TGGACGAAGAGCGGCGCGCTGACTTTCGCAAAAGCCGCGAGTCAAACTTTGCCATTGTACGAGATTCGGGCCGTTTTCGT
GTCAGTGCCTTCTACCAACGAGAGCTTCCTGGTGCGGTGATTCGCCGCATTGAAACCAATATTCCAACCTTTGAGGAACT
TAAGCTGCCTCATGTTTTGCAAGACTTAGCGATTGCCAAGCGTGGATTGGTGCTGGTGGTGGGGGCAACGGGCTCGGGTA
AATCGACCACCATGGCTGCGATGACAGGATATCGAAATACCCACCGTAGTGGACACATTTTGACGGTGGAAGATCCGATT
GAATTTGTCCACGAACATAAACGCTGTATTGTCACCCAGCGTGAAGTCGGTTTGGATACGGAAAGCTATGAAGTCGCACT
AAAGAACTCGCTGCGTCAAGCGCCAGATATGATTCTAATTGGTGAGATTCGCAGTCGGGAAACCATGGAATACGCCATGA
CCTTTGCTGAAACTGGTCACTTGTGTATGGCGACACTGCATGCCAATAACGCCAACCAAGCACTGGAGCGCATCTTACAC
TTGGTGCCTAAAGAACAAAAAGAGCAGTTTTTGTTTGACCTTTCAATGAACTTAAAAGGGGTAGTTGGTCAGCAGTTGAT
TCGTGATAAAAATGGTAAAGGTCGTCACGGTGTGTTCGAGGTGTTACTTAACAGCCCGCGCGTGTCGGATTTGATCCGTC
GTGGCGATTTGCATGAGCTGAAAATGACTATGGCGAAGTCGAAAGAAGTCGGTATGCAGACCTTTGACCAATCACTCTAT
ACATTAGTAGTGGAAGGCAAAATCAGCGAAGAAGACGCCATGCACAGTGCCGATTCGGCCAATGATTTACGTTTGATGCT
GAAGACGCAGCGAGGTGATATTAGTTCCCCCGGCTCATTGGCGAACGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3V4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

100

0.842
  pilU Pseudomonas stutzeri DSM 10701

59.429

95.109

0.565
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.753

88.043

0.394
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361

Multiple sequence alignment