Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LK04_RS02720 Genome accession   NZ_CP011427
Coordinates   569265..570257 (+) Length   330 a.a.
NCBI ID   WP_039327284.1    Uniprot ID   A0A0B1RBR6
Organism   Pantoea vagans strain ND02     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 564265..575257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK04_RS02685 (LK04_02680) - 564285..564554 (-) 270 WP_039327264.1 PTS sugar transporter subunit IIB -
  LK04_RS02690 (LK04_02685) - 564586..565038 (-) 453 WP_039327267.1 PTS sugar transporter subunit IIA -
  LK04_RS02695 (LK04_02690) - 565453..566187 (+) 735 WP_039327270.1 GntR family transcriptional regulator -
  LK04_RS02700 (LK04_02695) hemW 566225..567367 (-) 1143 WP_039327273.1 radical SAM family heme chaperone HemW -
  LK04_RS02705 (LK04_02700) - 567360..567953 (-) 594 WP_039327276.1 XTP/dITP diphosphatase -
  LK04_RS02710 (LK04_02705) - 567974..568528 (-) 555 WP_039327279.1 YggT family protein -
  LK04_RS02715 (LK04_02710) - 568541..569248 (-) 708 WP_039327282.1 YggS family pyridoxal phosphate-dependent enzyme -
  LK04_RS02720 (LK04_02715) pilT 569265..570257 (+) 993 WP_039327284.1 type IV pilus twitching motility protein PilT Machinery gene
  LK04_RS02725 (LK04_02720) ruvX 570388..570804 (-) 417 WP_039327287.1 Holliday junction resolvase RuvX -
  LK04_RS02730 (LK04_02725) - 570804..571367 (-) 564 WP_039327290.1 YqgE/AlgH family protein -
  LK04_RS02735 (LK04_02730) gshB 571450..572394 (-) 945 WP_039327293.1 glutathione synthase -
  LK04_RS02740 (LK04_02735) rsmE 572571..573302 (-) 732 WP_039327297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LK04_RS02745 (LK04_02740) endA 573379..574086 (-) 708 WP_059109767.1 deoxyribonuclease I -
  LK04_RS02750 (LK04_02745) - 574164..574676 (-) 513 WP_052206296.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 36099.35 Da        Isoelectric Point: 6.2666

>NTDB_id=145942 LK04_RS02720 WP_039327284.1 569265..570257(+) (pilT) [Pantoea vagans strain ND02]
MDLDEVVALSVKHNAADLHLCSGHLPHWRRQGALEPIPQQGELAADWLDAFIEKWMSGVQQEELEENGHVDFAITLHSGV
RLRANIFMQRQGLSLALRLIASRAPNLHSLQLPDVVQQLLQLEEGLILITGATGSGKSTTLAAMVDALNREQARHIITLE
DPIEFVHHSQRSLIQQREVGTHTASFQQALKAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGATQAVDR
LVDVFPAEEKNLVRTQLAGSLKAVLAQRLVPAIEGGRIGLFEVLVATPAVVNLIREGKMHQLPGVLQTGAQSGMQTFAQS
EQARRQSGMI

Nucleotide


Download         Length: 993 bp        

>NTDB_id=145942 LK04_RS02720 WP_039327284.1 569265..570257(+) (pilT) [Pantoea vagans strain ND02]
ATGGATTTGGATGAAGTAGTGGCCCTTAGTGTAAAGCATAACGCCGCCGATCTGCACCTTTGCAGCGGACATTTGCCTCA
CTGGCGACGACAAGGCGCACTGGAGCCGATTCCCCAGCAGGGCGAACTGGCTGCTGACTGGCTGGACGCGTTTATCGAGA
AGTGGATGAGTGGTGTGCAACAAGAAGAGTTGGAAGAGAACGGACACGTCGATTTCGCCATCACTCTACATAGCGGCGTG
CGACTGCGCGCCAATATCTTTATGCAGCGACAGGGCTTATCGCTGGCTCTGCGATTGATTGCCAGCCGTGCGCCCAATCT
ACACAGCCTGCAGCTTCCTGACGTGGTGCAGCAATTGCTGCAGTTGGAAGAAGGGCTGATTTTAATTACCGGTGCCACCG
GGAGTGGCAAATCCACCACGCTTGCGGCGATGGTGGATGCCCTTAACCGCGAACAAGCGCGCCATATCATTACGCTGGAA
GATCCGATTGAGTTTGTGCATCACAGCCAACGCTCACTGATTCAGCAACGGGAAGTCGGCACGCACACCGCCTCGTTTCA
ACAGGCGTTGAAAGCGGCGCTGCGTGAAGACCCTGATGTGATTCTGTTAGGGGAGTTGCGCGACAGTGAAACGATTCGTC
TGGCGCTGACCGCTGCTGAGACCGGGCATCTGGTGTTGGCAACCTTGCATACGCGCGGAGCGACACAGGCGGTGGATCGT
CTTGTTGATGTGTTTCCTGCAGAAGAGAAGAATCTGGTGCGTACCCAACTGGCGGGGAGCCTGAAAGCGGTGCTGGCGCA
GCGACTGGTGCCAGCCATCGAGGGCGGGCGAATAGGGCTGTTTGAGGTGCTGGTGGCGACGCCTGCGGTAGTGAATTTGA
TTCGTGAAGGGAAGATGCATCAGCTACCCGGCGTGCTGCAAACCGGCGCGCAATCGGGTATGCAGACCTTTGCGCAGAGC
GAACAGGCACGGCGACAGTCGGGGATGATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B1RBR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

49.245

100

0.494
  pilT Neisseria meningitidis 8013

49.245

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.379

97.576

0.482
  pilT Vibrio cholerae strain A1552

49.379

97.576

0.482
  pilT Acinetobacter baylyi ADP1

46.667

100

0.467
  pilT Pseudomonas stutzeri DSM 10701

46.667

100

0.467
  pilT Pseudomonas aeruginosa PAK

46.667

100

0.467
  pilT Acinetobacter baumannii D1279779

46.061

100

0.461
  pilT Acinetobacter nosocomialis M2

46.061

100

0.461
  pilT Acinetobacter baumannii strain A118

46.061

100

0.461
  pilT Legionella pneumophila strain ERS1305867

47.649

96.667

0.461
  pilT Legionella pneumophila strain Lp02

47.649

96.667

0.461
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.768

96.667

0.442
  pilU Pseudomonas stutzeri DSM 10701

41.745

97.273

0.406
  pilU Vibrio cholerae strain A1552

41.304

97.576

0.403
  pilU Acinetobacter baylyi ADP1

38.199

97.576

0.373

Multiple sequence alignment