Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAY24_RS12045 Genome accession   NZ_CP011412
Coordinates   2616360..2617475 (+) Length   371 a.a.
NCBI ID   WP_046859888.1    Uniprot ID   A0A0F7K036
Organism   Sedimenticola thiotaurini strain SIP-G1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2611360..2622475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAY24_RS12030 (AAY24_12030) - 2613322..2614425 (-) 1104 WP_046859886.1 efflux RND transporter periplasmic adaptor subunit -
  AAY24_RS12035 (AAY24_12035) - 2614418..2615146 (-) 729 WP_082117130.1 TetR/AcrR family transcriptional regulator -
  AAY24_RS12040 (AAY24_12040) - 2615354..2616154 (-) 801 WP_046859887.1 MOSC domain-containing protein -
  AAY24_RS12045 (AAY24_12045) pilU 2616360..2617475 (+) 1116 WP_046859888.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAY24_RS12050 (AAY24_12050) - 2617507..2617776 (+) 270 WP_046859889.1 GIY-YIG nuclease family protein -
  AAY24_RS12055 (AAY24_12055) - 2617808..2618095 (+) 288 WP_046861271.1 YcgL domain-containing protein -
  AAY24_RS12060 (AAY24_12060) serB 2618279..2619490 (+) 1212 WP_046859890.1 phosphoserine phosphatase SerB -
  AAY24_RS12065 (AAY24_12065) - 2619525..2619959 (+) 435 WP_046859891.1 DUF411 domain-containing protein -
  AAY24_RS12070 (AAY24_12070) djlA 2620100..2620912 (-) 813 WP_046859892.1 co-chaperone DjlA -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40991.08 Da        Isoelectric Point: 6.0102

>NTDB_id=145715 AAY24_RS12045 WP_046859888.1 2616360..2617475(+) (pilU) [Sedimenticola thiotaurini strain SIP-G1]
MEIIPYLKLMVQKNGSDLFFSTGAPPNLKAEGRTMPIGDTPMGPGAVRKLAYSIMSDDQISEFEGTMECNLAVSMKGLGR
FRVNVFRQRGDASMVIRYIKGDIPTIEELNLPPSLKDLIMMPRGLILVVGSTGSGKSTSLASMIDHRNASTTGHILTIED
PVEFLHTHKKSVVDQREVGLDTLSYEVALKNAMREAPDVILIGEIRDRQTMQHAIAYAETGHLCLSTLHANNANQALDRI
VNFFPDSAHHQLFMDLSLNLKAVISQRLVKSKDGRRVPAVEIMLLTTFVAELIQKGDIHAVKEAMEQGNERGMQTFDQAL
YKLYKEGKITLEEALANADSRNNLSLKIRLSESGNVDGDNAAMAISDEEFF

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=145715 AAY24_RS12045 WP_046859888.1 2616360..2617475(+) (pilU) [Sedimenticola thiotaurini strain SIP-G1]
ATGGAGATCATACCCTACCTGAAGCTGATGGTTCAGAAAAATGGCTCTGACCTGTTTTTCAGCACAGGTGCCCCCCCCAA
CCTGAAGGCCGAGGGACGCACCATGCCGATCGGTGACACACCGATGGGGCCGGGTGCGGTACGGAAACTTGCCTACTCGA
TCATGAGTGACGACCAGATCTCCGAGTTTGAAGGTACCATGGAGTGCAACCTGGCGGTCTCCATGAAAGGACTGGGACGT
TTCCGCGTCAACGTATTCCGGCAACGGGGTGACGCCTCCATGGTGATCCGCTATATCAAGGGCGATATCCCCACCATCGA
AGAGCTGAACCTGCCCCCGTCCCTTAAAGATCTGATTATGATGCCACGCGGCCTGATCCTGGTGGTGGGCTCCACCGGCT
CCGGTAAGTCCACCTCCCTGGCCTCCATGATCGATCACCGTAACGCCAGCACCACCGGCCATATCCTCACTATTGAGGAC
CCGGTGGAGTTCCTGCACACCCATAAAAAATCGGTGGTAGACCAGCGGGAAGTGGGGCTGGATACCCTCTCCTACGAAGT
GGCGCTGAAAAATGCCATGCGTGAGGCGCCGGATGTGATTCTGATTGGCGAGATTCGTGACCGCCAGACCATGCAGCACG
CCATCGCCTACGCCGAAACCGGCCATCTCTGTCTCTCCACCCTGCACGCCAACAACGCCAACCAGGCATTGGACCGCATC
GTCAACTTCTTCCCCGATTCGGCCCATCACCAGCTGTTCATGGACCTGTCGCTGAACCTGAAAGCGGTCATATCCCAGCG
TCTGGTCAAGAGCAAGGATGGCCGACGGGTGCCGGCGGTGGAGATCATGCTGCTCACCACCTTCGTGGCGGAACTGATCC
AGAAGGGCGATATCCACGCGGTCAAGGAGGCCATGGAACAGGGCAATGAGCGGGGCATGCAGACCTTTGACCAGGCACTG
TACAAACTCTACAAGGAGGGCAAGATCACCCTGGAAGAGGCGCTGGCCAATGCCGATTCACGCAACAACCTGTCGTTGAA
GATCCGTCTCTCCGAGAGTGGCAACGTGGATGGCGACAATGCCGCCATGGCCATCTCCGACGAGGAGTTCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F7K036

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.125

94.609

0.531
  pilU Acinetobacter baylyi ADP1

51.657

97.574

0.504
  pilU Vibrio cholerae strain A1552

50.281

95.957

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.248

91.375

0.404
  pilT Acinetobacter baylyi ADP1

42.941

91.644

0.394
  pilT Pseudomonas aeruginosa PAK

41.86

92.722

0.388
  pilT Legionella pneumophila strain Lp02

43.03

88.949

0.383
  pilT Legionella pneumophila strain ERS1305867

43.03

88.949

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.988

92.722

0.38
  pilT Neisseria meningitidis 8013

39.943

93.801

0.375
  pilT Vibrio cholerae strain A1552

41.441

89.757

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

89.757

0.372
  pilT Acinetobacter nosocomialis M2

40.237

91.105

0.367
  pilT Acinetobacter baumannii D1279779

40.237

91.105

0.367
  pilT Acinetobacter baumannii strain A118

40.237

91.105

0.367
  pilT Neisseria gonorrhoeae MS11

39.193

93.531

0.367

Multiple sequence alignment