Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FORC14_RS13890 Genome accession   NZ_CP011406
Coordinates   2988364..2989470 (-) Length   368 a.a.
NCBI ID   WP_053342934.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_014     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2983364..2994470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC14_RS13860 (FORC14_2724) - 2984018..2984515 (+) 498 WP_020835372.1 SprT family zinc-dependent metalloprotease -
  FORC14_RS13865 (FORC14_2725) endA 2984635..2985330 (+) 696 WP_063517769.1 deoxyribonuclease I -
  FORC14_RS13870 (FORC14_2726) rsmE 2985455..2986186 (+) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FORC14_RS13875 (FORC14_2727) gshB 2986201..2987151 (+) 951 WP_005425823.1 glutathione synthase -
  FORC14_RS13880 (FORC14_2728) - 2987265..2987828 (+) 564 WP_005461717.1 YqgE/AlgH family protein -
  FORC14_RS13885 (FORC14_2729) ruvX 2987877..2988302 (+) 426 WP_017449131.1 Holliday junction resolvase RuvX -
  FORC14_RS13890 (FORC14_2730) pilU 2988364..2989470 (-) 1107 WP_053342934.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FORC14_RS13895 (FORC14_2731) pilT 2989501..2990541 (-) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  FORC14_RS13900 (FORC14_2732) - 2990570..2991283 (+) 714 WP_025557492.1 YggS family pyridoxal phosphate-dependent enzyme -
  FORC14_RS13905 (FORC14_2733) proC 2991403..2992221 (+) 819 WP_005482485.1 pyrroline-5-carboxylate reductase -
  FORC14_RS13910 (FORC14_2734) - 2992274..2992831 (+) 558 WP_005482440.1 YggT family protein -
  FORC14_RS13915 (FORC14_2735) yggU 2992831..2993121 (+) 291 WP_005482461.1 DUF167 family protein YggU -
  FORC14_RS13920 (FORC14_2736) - 2993249..2993680 (+) 432 WP_063517770.1 DUF4426 domain-containing protein -
  FORC14_RS13925 (FORC14_2737) - 2993798..2994400 (+) 603 WP_063517771.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41414.67 Da        Isoelectric Point: 7.2515

>NTDB_id=145650 FORC14_RS13890 WP_053342934.1 2988364..2989470(-) (pilU) [Vibrio parahaemolyticus strain FORC_014]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAKLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETIIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVIGQQLIRNKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEPFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=145650 FORC14_RS13890 WP_053342934.1 2988364..2989470(-) (pilU) [Vibrio parahaemolyticus strain FORC_014]
ATGGATCTGAATAAATTTCTTGAAGGTATGTTGACGCTAAAAGCGTCGGATCTTTACATCACGGTTGGTGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAGAAACTCACCGAACATGATGTTGCAAAGTTGCTTGATAGCGCAA
TGGATCCGGATCGGCGTCAGGAGTTTCGCAAAAGCCGCGAATCAAACTTTGCTATCGTCAGAGATTGTGGTCGCTTCCGT
GTTAGTGCTTTTTTTCAGCGAGAACTGCCGGGTGCAGTTATTCGCCGTATCGAAACCATCATTCCTACTTTTGAGCAGTT
AAAACTGCCTTTAGTGCTGCAAGACTTAGCGATTGCCAAGCGTGGTTTGGTGCTGGTGGTCGGCGCGACTGGCTCGGGTA
AATCGACCACGATGGCGGCGATGACGGGCTATCGAAACAGCAACAAAACCGGTCACATCTTGACGGTCGAAGATCCGATC
GAATTTGTGCATGAGCATAAGCGTTGTATCGTGACTCAGCGTGAGGTGGGGTTGGATACCGAGAGCTATGAAGTCGCATT
GAAAAACTCGCTGCGCCAAGCGCCAGATATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACTTGTGTATGGCAACGTTGCACGCCAACAACGCCAACCAAGCGTTAGAACGTATTCTCCAT
TTGGTGCCTAAAGATCAAAAAGAGCAATTCTTGTTTGATTTGTCGATGAACCTAAAAGGCGTGATTGGTCAGCAGCTGAT
CCGTAATAAGAATGGTCAAGGGCGTCATGGTGTGTTTGAGATCTTGCTAAACAGCCCACGAGTGTCGGATTTGATCCGTC
GTGGTGATTTACACGAATTGAAATCAACCATGGCACGATCCAATGAGTTTGGCATGCTGACGTTTGACCAATCGCTCTAC
AAGTTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTGCACAGTGCGGATTCTGCCAACGATCTTCGTTTAATGCT
GAAAACACAGCGTGGTGAGCCGTTCTCTACAGGCAGTCTTGCCAACGTCAAAATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58

95.109

0.552
  pilU Acinetobacter baylyi ADP1

55.271

95.38

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

92.12

0.405
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Acinetobacter nosocomialis M2

42.478

92.12

0.391
  pilT Acinetobacter baumannii D1279779

42.478

92.12

0.391
  pilT Acinetobacter baumannii strain A118

42.478

92.12

0.391
  pilT Pseudomonas aeruginosa PAK

42.183

92.12

0.389
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

41.994

89.946

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.361

90.217

0.364
  pilT Neisseria meningitidis 8013

40.361

90.217

0.364

Multiple sequence alignment