Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VL20_RS05080 Genome accession   NZ_CP011339
Coordinates   1030871..1031971 (+) Length   366 a.a.
NCBI ID   WP_052275796.1    Uniprot ID   A0A0K1RXC0
Organism   Microcystis panniformis FACHB-1757     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1025871..1036971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VL20_RS05065 (VL20_1242) - 1026959..1027618 (+) 660 WP_052275793.1 HAD-IA family hydrolase -
  VL20_RS05070 (VL20_1243) grpE 1027754..1028476 (-) 723 WP_052275794.1 nucleotide exchange factor GrpE -
  VL20_RS05075 (VL20_1244) pilB/pilB1 1028717..1030732 (+) 2016 WP_052275795.1 GspE/PulE family protein Machinery gene
  VL20_RS05080 (VL20_1245) pilT 1030871..1031971 (+) 1101 WP_052275796.1 type IV pilus twitching motility protein PilT Machinery gene
  VL20_RS32275 - 1031921..1032226 (-) 306 WP_284526007.1 hypothetical protein -
  VL20_RS05085 (VL20_1246) - 1032324..1033311 (+) 988 Protein_1062 type II secretion system F family protein -
  VL20_RS05090 (VL20_1249) - 1033408..1033944 (+) 537 WP_052275797.1 hypothetical protein -
  VL20_RS05095 (VL20_1250) - 1034181..1036751 (-) 2571 WP_052275798.1 TIGR03960 family B12-binding radical SAM protein -
  VL20_RS32280 (VL20_1251) - 1036783..1036908 (+) 126 WP_284526008.1 hypothetical protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40374.59 Da        Isoelectric Point: 8.2594

>NTDB_id=144701 VL20_RS05080 WP_052275796.1 1030871..1031971(+) (pilT) [Microcystis panniformis FACHB-1757]
MELMIEDLMQQLVEMGGSDMHIQSGAPVYFRISGKLAPINEESLTPQDCQKLIFSMLNNTQRKELEQNWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEQLNLPRVVRELSERPRGLILVTGQTGSGKTTTLAAILDLINRTRSEHILTV
EDPIEYVFTNIKSLFHQRQKGEDTKSFANALRASLREDPDIILVGEMRDLETISLAITAAETGHLVFGTLHTNSAAGTVD
RIIDVFPANQQAQIRAMLSNSLLAVFSQNLVKKKNPKPGEFGRAMVQEILVVTPAIANLIREGKAPQIYSAIQTGMKLGM
QTMEQGLANLVVRGVVSLEEAISKSGKPDELQRLIAGASTNAKARI

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=144701 VL20_RS05080 WP_052275796.1 1030871..1031971(+) (pilT) [Microcystis panniformis FACHB-1757]
ATGGAATTAATGATCGAAGATTTAATGCAACAATTGGTCGAGATGGGCGGTTCTGATATGCACATTCAGTCTGGGGCGCC
AGTCTATTTTCGCATTAGTGGCAAACTCGCCCCGATTAATGAAGAATCTTTAACCCCGCAAGATTGTCAGAAATTGATTT
TTAGTATGCTCAATAATACTCAAAGAAAAGAGTTAGAGCAGAATTGGGAATTAGACTGTTCCTACGGGGTGAAAGGATTA
GCAAGATTTAGGGTCAATGTTTATAAAGAAAGGGGGGCTTATGCAGCTTGTTTACGGGCTTTGTCCTCTAAAATTCCTAA
CTTTGAACAATTAAATTTACCTAGAGTAGTACGGGAGTTATCGGAAAGACCGCGGGGATTAATTCTCGTAACTGGCCAAA
CAGGTTCGGGTAAAACTACCACTTTAGCGGCAATTTTAGACTTAATTAATCGGACTAGGTCTGAACATATTTTAACTGTA
GAAGACCCAATTGAATACGTTTTTACTAATATAAAAAGTCTGTTTCACCAACGACAAAAAGGAGAAGATACCAAGAGTTT
TGCTAACGCTTTAAGAGCATCTTTACGGGAAGATCCTGATATTATCCTTGTGGGAGAAATGCGGGATTTAGAAACAATTA
GTTTGGCAATCACGGCGGCAGAAACCGGTCACTTAGTCTTCGGAACCCTGCACACCAACTCAGCAGCGGGAACAGTGGAT
CGGATCATTGACGTTTTTCCAGCTAACCAACAGGCACAAATTCGCGCCATGTTATCCAATTCTTTACTAGCAGTTTTTAG
TCAAAACCTAGTCAAGAAAAAAAATCCTAAACCGGGGGAATTTGGCCGGGCAATGGTACAGGAAATCCTGGTGGTAACTC
CAGCGATCGCTAACCTAATTCGGGAAGGAAAAGCCCCCCAAATCTACTCAGCAATTCAAACGGGAATGAAGTTAGGAATG
CAAACCATGGAACAGGGATTAGCTAATTTAGTTGTTAGGGGTGTTGTCTCTTTGGAAGAGGCCATTTCTAAAAGCGGTAA
ACCCGATGAATTACAACGTCTTATCGCCGGGGCCAGCACTAACGCTAAAGCCCGGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1RXC0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

52.174

94.262

0.492
  pilT Legionella pneumophila strain ERS1305867

52.174

94.262

0.492
  pilT Pseudomonas aeruginosa PAK

51.009

94.809

0.484
  pilT Acinetobacter baumannii D1279779

50.72

94.809

0.481
  pilT Acinetobacter nosocomialis M2

50.72

94.809

0.481
  pilT Acinetobacter baumannii strain A118

50.72

94.809

0.481
  pilT Acinetobacter baylyi ADP1

50.144

94.809

0.475
  pilT Pseudomonas stutzeri DSM 10701

50.144

94.809

0.475
  pilT Vibrio cholerae strain A1552

50

92.896

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

92.896

0.464
  pilT Neisseria meningitidis 8013

49.853

92.623

0.462
  pilT Neisseria gonorrhoeae MS11

49.558

92.623

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.197

96.995

0.448
  pilU Pseudomonas stutzeri DSM 10701

38.611

98.361

0.38
  pilU Vibrio cholerae strain A1552

39.233

92.623

0.363

Multiple sequence alignment