Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   VL20_RS05075 Genome accession   NZ_CP011339
Coordinates   1028717..1030732 (+) Length   671 a.a.
NCBI ID   WP_052275795.1    Uniprot ID   A0A0K1RWY8
Organism   Microcystis panniformis FACHB-1757     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1023717..1035732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VL20_RS05045 (VL20_1239) - 1023902..1024297 (-) 396 WP_002738951.1 PH domain-containing protein -
  VL20_RS05050 (VL20_1240) rnpA 1024287..1024649 (-) 363 WP_002738574.1 ribonuclease P protein component -
  VL20_RS05055 rpmH 1024676..1024813 (-) 138 WP_002739276.1 50S ribosomal protein L34 -
  VL20_RS05060 (VL20_1241) - 1024905..1026893 (+) 1989 WP_052275792.1 amylo-alpha-1,6-glucosidase -
  VL20_RS05065 (VL20_1242) - 1026959..1027618 (+) 660 WP_052275793.1 HAD-IA family hydrolase -
  VL20_RS05070 (VL20_1243) grpE 1027754..1028476 (-) 723 WP_052275794.1 nucleotide exchange factor GrpE -
  VL20_RS05075 (VL20_1244) pilB/pilB1 1028717..1030732 (+) 2016 WP_052275795.1 GspE/PulE family protein Machinery gene
  VL20_RS05080 (VL20_1245) pilT 1030871..1031971 (+) 1101 WP_052275796.1 type IV pilus twitching motility protein PilT Machinery gene
  VL20_RS32275 - 1031921..1032226 (-) 306 WP_284526007.1 hypothetical protein -
  VL20_RS05085 (VL20_1246) - 1032324..1033311 (+) 988 Protein_1062 type II secretion system F family protein -
  VL20_RS05090 (VL20_1249) - 1033408..1033944 (+) 537 WP_052275797.1 hypothetical protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75040.07 Da        Isoelectric Point: 6.0496

>NTDB_id=144700 VL20_RS05075 WP_052275795.1 1028717..1030732(+) (pilB/pilB1) [Microcystis panniformis FACHB-1757]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGADQMQQALVETRKSGRSLVEILQQLTGRPLPPDLQRQYKKNQLFELKIL
YGVESIDPELSDVDGLEIARLIDSLIPIDLCRRYRLIPLRKIEGEPAAILIAMVDPDNLEASDDINRILRPRELGLQRLV
ITSEDYERLLEKFHSAQSELEQEKARLQKEKELEKLSDLTGIVSNIEVASGAANDEVADDLGEGEANQAPVINLVNKIIA
KALQEGTSDIHIEPQEEFLKIRFRKDGVLHQAFDPLPKRITPAVTARLKIMAELDIAERRLPQDGKIRRMYQGRKVDFRV
NTLPSRYGEKVCLRILDNSATQLGLDKLITDQKTLQIVRDMASRPFGLLLVTGPTGSGKSTTLYSVLAERNHPGVNISTA
EDPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
EMGVEPFMISGSLLGVLAQRLVRRVCSQCRVAYHPSQEELARFGLSAANEREVTFYKANTLTLEEIQQARKQGNLCLKCS
GSGYKGRIGVYEVMQNSERLQQLINQGATTDRIKETAVDEGMVTLLAYSLNLVREGYTTLEEVERVTFTDSGLEAELKAK
RKSGLICRGCRAELQPEWLDCPYCMTPRFSD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=144700 VL20_RS05075 WP_052275795.1 1028717..1030732(+) (pilB/pilB1) [Microcystis panniformis FACHB-1757]
ATGACATTTTCTTCCAAGAAAACCCGTGCCGTTGCCCTACGCAATTACTTTTCCCCTTTTGGTAACAAGTTGGTGCAGTC
GGGATATATCGGTGCCGACCAAATGCAACAGGCCCTGGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAC
AACAACTAACTGGGCGCCCTTTGCCCCCGGATTTGCAGCGACAGTACAAGAAAAATCAGTTATTTGAATTAAAAATTCTC
TACGGAGTCGAATCGATCGACCCCGAATTAAGCGATGTGGATGGACTGGAAATAGCCCGATTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCCCTGCGAAAGATCGAAGGAGAGCCGGCTGCGATTTTGATAGCCATGG
TCGATCCCGACAACCTCGAGGCTTCCGACGATATTAATCGTATTCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTGGTG
ATTACTAGCGAAGATTATGAAAGATTACTGGAAAAATTCCACTCGGCTCAATCGGAATTAGAACAGGAAAAAGCCCGACT
GCAAAAAGAAAAAGAACTAGAAAAACTCTCGGATCTCACTGGTATTGTCAGCAATATTGAGGTGGCCTCTGGTGCGGCTA
ACGATGAAGTGGCCGATGATCTCGGAGAAGGGGAAGCTAATCAAGCTCCCGTCATCAACCTCGTCAATAAAATTATAGCT
AAAGCCCTACAGGAAGGGACTTCCGATATTCACATCGAACCCCAAGAAGAATTCCTGAAAATTCGCTTTCGCAAAGATGG
AGTTCTTCATCAAGCTTTTGATCCACTGCCGAAAAGAATTACCCCGGCGGTGACAGCCCGTCTTAAAATCATGGCCGAGC
TCGATATCGCCGAACGTCGTCTTCCCCAAGATGGTAAAATTCGGCGGATGTATCAAGGGCGGAAAGTGGATTTTCGGGTT
AATACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTACGGATTCTCGATAACTCGGCTACCCAGTTGGGTTTAGATAA
ACTAATCACCGACCAAAAGACCCTGCAAATCGTCCGAGACATGGCCAGTCGTCCCTTTGGTCTGTTGCTGGTGACAGGCC
CGACCGGTTCGGGGAAATCCACCACTTTATACTCGGTGTTAGCCGAAAGAAATCACCCCGGGGTCAATATTAGTACGGCC
GAAGATCCGATCGAATATTCTCTACCGGGCATCACCCAGGTGCAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGAT
TCTGCGGGCATTCATGCGACAAGACCCGGATGTGATTCTGGTGGGAGAAACTAGGGACAAGGAAACGGCCAAAACAGCGA
TCGAGGCGGCCTTAACTGGTCACTTGGTCCTAACTACCCTACACACCAACGATGCCGCAGGTGCGATCGCTCGTCTCGAT
GAGATGGGGGTTGAACCGTTTATGATTTCTGGTTCTCTCTTGGGAGTCTTGGCCCAGCGTCTCGTGCGGCGCGTCTGTAG
CCAGTGTCGAGTCGCCTATCACCCCAGTCAAGAGGAATTAGCCCGTTTTGGTCTTTCCGCCGCCAATGAACGGGAAGTGA
CCTTTTATAAGGCTAATACCCTCACCCTTGAGGAAATTCAACAGGCGCGAAAACAGGGCAATCTCTGCCTTAAGTGTAGC
GGCAGCGGTTATAAAGGTCGCATTGGTGTCTATGAGGTAATGCAGAATAGTGAACGACTACAGCAGTTAATTAACCAAGG
GGCAACCACTGATCGCATTAAGGAGACGGCCGTCGATGAGGGCATGGTGACACTCCTAGCCTATAGTTTAAATCTGGTGC
GCGAGGGTTACACCACCCTCGAAGAAGTGGAACGGGTGACATTTACCGATTCCGGTCTGGAAGCGGAACTAAAAGCCAAA
CGGAAAAGCGGTTTAATCTGTCGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGACACC
GCGATTTAGTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(237-504)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1RWY8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

73.112

98.659

0.721
  pilF Thermus thermophilus HB27

40.426

91.058

0.368

Multiple sequence alignment