Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/smf   Type   Machinery gene
Locus tag   BW25113_RS17035 Genome accession   NZ_CP009273
Coordinates   3425795..3426919 (-) Length   374 a.a.
NCBI ID   WP_000228551.1    Uniprot ID   A0A024KZR4
Organism   Escherichia coli BW25113 strain K-12     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3420795..3431919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BW25113_RS17005 (BW25113_3279) yrdA 3422595..3423149 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  BW25113_RS17010 (BW25113_3280) yrdB 3423125..3423382 (-) 258 WP_001070563.1 DUF1488 domain-containing protein -
  BW25113_RS17015 (BW25113_3281) aroE 3423379..3424197 (-) 819 WP_000451243.1 shikimate dehydrogenase -
  BW25113_RS17020 (BW25113_3282) tsaC 3424202..3424774 (-) 573 WP_001301412.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  BW25113_RS17025 (BW25113_3283) yrdD 3424779..3425321 (-) 543 WP_001129722.1 type I DNA topoisomerase -
  BW25113_RS17030 (BW25113_3284) smg 3425350..3425823 (-) 474 WP_000460680.1 DUF494 family protein Smg -
  BW25113_RS17035 (BW25113_4473) dprA/smf 3425795..3426919 (-) 1125 WP_000228551.1 DNA-protecting protein DprA Machinery gene
  BW25113_RS17040 (BW25113_3287) def 3427049..3427558 (+) 510 WP_000114984.1 peptide deformylase -
  BW25113_RS17045 (BW25113_3288) fmt 3427573..3428520 (+) 948 WP_000004473.1 methionyl-tRNA formyltransferase -
  BW25113_RS17050 (BW25113_3289) rsmB 3428566..3429855 (+) 1290 WP_000744778.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  BW25113_RS17055 (BW25113_3290) trkA 3429877..3431253 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  BW25113_RS17060 (BW25113_3291) mscL 3431383..3431793 (+) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sxy/tfoX dprA/smf positive effect
  sxy/tfoX fimT/comN/ppdA positive effect
  sxy/tfoX comE1/ybaV positive effect
  sxy/tfoX pilN/comB/hofN positive effect
  sxy/tfoX pilO/comC/hofO positive effect
  sxy/tfoX pilB/hofB positive effect
  sxy/tfoX pilD/pppA positive effect
  sxy/tfoX rec2/ycaI positive effect
  sxy/tfoX pilP/comD/hofP positive effect
  sxy/tfoX comF/gntX positive effect
  sxy/tfoX pilM/comA/hofM positive effect
  sxy/tfoX pilA/ppdD positive effect
  sxy/tfoX fimU/comO/ppdB positive effect
  sxy/tfoX comP/ygdB positive effect
  sxy/tfoX pilV/comQ/ppdC positive effect
  sxy/tfoX ssb positive effect
  sxy/tfoX mshC/yggT positive effect
  sxy/tfoX pilQ/comE/hofQ positive effect
  sxy/tfoX pilT/yggR positive effect
  sxy/tfoX pilC/hofC positive effect
  sxy/tfoX pilB/ycgB positive effect
  sxy/tfoX comM/yifB positive effect

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40954.92 Da        Isoelectric Point: 6.3122

>NTDB_id=1445 BW25113_RS17035 WP_000228551.1 3425795..3426919(-) (dprA/smf) [Escherichia coli BW25113 strain K-12]
MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=1445 BW25113_RS17035 WP_000228551.1 3425795..3426919(-) (dprA/smf) [Escherichia coli BW25113 strain K-12]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG
GGTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (DprA_WH)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024KZR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.238

98.663

0.476
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

45.104

90.107

0.406
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39

Multiple sequence alignment