Detailed information NTG0001439

Bioinformatically predicted

Overview

• Name: rec2/ycaI
• Type: Machinery gene
• Locus tag: BW25113_RS04735
• Genome accession: NZ_CP009273
• Coordinates: 959776..962040 (+)
• Length: 754 a.a.
• NCBI ID: WP_000705764.1
• Uniprot ID: -
• Organism: Escherichia coli BW25113 strain K-12
• Function: ssDNA transport through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 954776..967040

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BW25113_RS04715 (BW25113_0909)	ycaL	955720..956484 (+)	765	WP_001350496.1	metallopeptidase YcaL	-
BW25113_RS04720 (BW25113_0910)	cmk	956657..957340 (+)	684	WP_000125016.1	(d)CMP kinase	-
BW25113_RS04725 (BW25113_0911)	rpsA	957451..959124 (+)	1674	WP_000140327.1	30S ribosomal protein S1	-
BW25113_RS04730 (BW25113_0912)	ihfB	959284..959568 (+)	285	WP_000167336.1	integration host factor subunit beta	-
BW25113_RS04735 (BW25113_0913)	rec2/ycaI	959776..962040 (+)	2265	WP_000705764.1	ComEC family protein	Machinery gene
BW25113_RS04740 (BW25113_0914)	msbA	962077..963825 (+)	1749	WP_000551270.1	lipid A ABC transporter ATP-binding protein/permease MsbA	-
BW25113_RS04745 (BW25113_0915)	lpxK	963822..964808 (+)	987	WP_000570539.1	tetraacyldisaccharide 4'-kinase	-
BW25113_RS04750 (BW25113_0916)	ycaQ	964845..966077 (+)	1233	WP_000056538.1	crosslink repair DNA glycosylase YcaQ	-
BW25113_RS04755 (BW25113_0917)	ycaR	966129..966311 (+)	183	WP_000350058.1	protein YcaR	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
sxy/tfoX	rec2/ycaI	positive effect
sxy/tfoX	fimT/comN/ppdA	positive effect
sxy/tfoX	comE1/ybaV	positive effect
sxy/tfoX	pilN/comB/hofN	positive effect
sxy/tfoX	pilO/comC/hofO	positive effect
sxy/tfoX	pilB/hofB	positive effect
sxy/tfoX	pilD/pppA	positive effect
sxy/tfoX	pilP/comD/hofP	positive effect
sxy/tfoX	comF/gntX	positive effect
sxy/tfoX	pilM/comA/hofM	positive effect
sxy/tfoX	pilA/ppdD	positive effect
sxy/tfoX	fimU/comO/ppdB	positive effect
sxy/tfoX	comP/ygdB	positive effect
sxy/tfoX	pilV/comQ/ppdC	positive effect
sxy/tfoX	ssb	positive effect
sxy/tfoX	mshC/yggT	positive effect
sxy/tfoX	pilQ/comE/hofQ	positive effect
sxy/tfoX	dprA/smf	positive effect
sxy/tfoX	pilT/yggR	positive effect
sxy/tfoX	pilC/hofC	positive effect
sxy/tfoX	pilB/ycgB	positive effect
sxy/tfoX	comM/yifB	positive effect

Sequence

Protein

• Length: 754 a.a.
• Molecular weight: 84474.03 Da
• Isoelectric Point: 8.4858

>NTDB_id=1439 BW25113_RS04735 WP_000705764.1 959776..962040(+) (rec2/ycaI) [Escherichia coli BW25113 strain K-12]
MKITTVGVCIISGIFPLLILPQLPGTLTLAFLTLFACVLAFIPVKTVRYIALTLLFFVWGILSAKQILWAGETLTGATQD
AIVEITATDGMTTHYGQITHLQGRRIFPASGLVMYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRDTGTAHLMAISGLHIAFAALLAA
GLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTGMQPPALRTMVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPV
AILSQSLWLSAAAVAALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANLLAIPLVTF
ITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIAECWQWLSFSPWFLLVVWRLNAWRTLPAMCV
AGGLLMCWPLWQKPRPDEWQLYMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHL
DHRGGLDSILHIWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVKVDDGTNSILLTGDIEA
PAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKVALASASRYNAWRLPSNKVKHRYQLQGYQWIDTPHQGQT
TVNFSAQGWRISSLREQILPRWYHQWFGVPVDNG

Nucleotide

• Length: 2265 bp

>NTDB_id=1439 BW25113_RS04735 WP_000705764.1 959776..962040(+) (rec2/ycaI) [Escherichia coli BW25113 strain K-12]
ATGAAAATAACGACAGTCGGTGTATGCATAATTAGCGGAATTTTTCCGTTGCTGATTTTGCCCCAATTGCCTGGGACATT
AACCCTTGCGTTTCTGACTCTCTTCGCCTGCGTACTGGCATTTATCCCTGTTAAAACCGTCCGTTATATCGCGCTGACGT
TGCTGTTTTTCGTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGAT
GCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGTATATT
CCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCA
AAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATAGCCAGCGTTATGCCATTGCCCAGCATCAGCCGCTC
ACCGGCCGTTTTCTGCAGGCAAGTGTCATTGAACCGAATTGTAGCCTGCGTGCACAGTATCTGGCGTCACTACAAACAAC
GCTGCAACCCTATCCGTGGAATGCGGTTATTCTTGGTTTAGGTATGGGGGAACGGTTATCCGTCCCCAAAGAAATCAAAA
ATATCATGCGCGATACTGGAACGGCGCATTTAATGGCGATATCGGGATTGCACATCGCTTTTGCGGCGTTGCTGGCTGCC
GGACTCATTCGCAGTGGACAAATTTTTCTGCCTGGGCGCTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTG
TGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAATGC
TTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTT
GCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTCGCGGCATTGATATTTTGGTATCAGTGGTTTCCCTGTCC
TGAGTGGCAACTGCCGCCGGTATTGCGTGCAGTTGTTTCCCTCATCCATCTGCAACTGGGAATCACACTTCTGCTTATGC
CCGTGCAAATCGTCATATTTCATGGCATTAGTCTGACCTCGTTTATTGCAAATCTATTAGCAATTCCCTTGGTGACATTT
ATCACGGTTCCGTTGATCCTCGCCGCGATGGTTGTGCATTTAAGCGGGCCGTTAATCCTGGAGCAAGGGTTATGGTTTCT
TGCCGACCGGTCTTTGGCTTTACTTTTCTGGGGGTTAAAGAGTTTGCCGGAAGGGTGGATCAACATTGCTGAATGTTGGC
AATGGCTATCATTTTCCCCATGGTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTG
GCTGGAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCTTGATGTCGG
GCAAGGGCTGGCAATGGTGATAGCCAGAAACGGCAAAGCGATTCTCTATGACACAGGACTGGCCTGGCCTGAAGGGGATA
GTGGGCAACAACTGATTATCCCCTGGCTCCACTGGCATAATCTTGAACCGGAAGGCGTTATTCTGAGCCATGAACATCTG
GATCACCGGGGAGGGCTGGATTCAATATTGCATATATGGCCGATGTTATGGATCAGAAGTCCGTTAAACTGGGAACATCA
TCAGCCCTGTGTGCGTGGCGAAGCGTGGCAATGGCAAGGATTGCGTTTCAGCGCGCACTGGCCTTTACAAGGTAGCAACG
ATAAAGGAAATAACCATTCCTGTGTGGTTAAGGTTGATGACGGGACGAATAGCATTCTTCTAACCGGTGATATTGAAGCC
CCAGCTGAACAAAAGATGCTAAGCCGTTACTGGCAGCAAGTGCAGGCAACATTGCTTCAGGTACCTCACCATGGCAGTAA
TACCTCATCATCGTTGCCATTAATTCAGCGAGTGAATGGAAAAGTGGCACTCGCATCGGCATCGCGCTATAACGCATGGC
GACTGCCCTCTAACAAAGTTAAGCATCGCTATCAACTGCAAGGATATCAATGGATTGATACTCCACATCAAGGTCAAACA
ACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTT
TGGCGTGCCAGTGGATAACGGGTAG

Domains

Predicted by InterproScan.

• Competence protein (Competence) (201-466)

• Metallo-beta-lactamase superfamily (Lactamase_B) (505-683)