Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC/hofC   Type   Machinery gene
Locus tag   BW25113_RS00525 Genome accession   NZ_CP009273
Coordinates   111009..112211 (-) Length   400 a.a.
NCBI ID   WP_000157266.1    Uniprot ID   A0A037YRV8
Organism   Escherichia coli BW25113 strain K-12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 106009..117211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BW25113_RS00495 (BW25113_0099) mutT 107531..107920 (+) 390 WP_000736007.1 8-oxo-dGTP diphosphatase MutT -
  BW25113_RS00505 (BW25113_0101) yacG 108136..108333 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  BW25113_RS00510 (BW25113_0102) zapD 108343..109086 (-) 744 WP_001194734.1 cell division protein ZapD -
  BW25113_RS00515 (BW25113_0103) coaE 109086..109706 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  BW25113_RS25965 - 109731..109775 (+) 45 WP_120795372.1 protein YacM -
  BW25113_RS00520 (BW25113_0104) guaC 109931..110974 (+) 1044 WP_001217338.1 GMP reductase -
  BW25113_RS00525 (BW25113_0106) pilC/hofC 111009..112211 (-) 1203 WP_000157266.1 protein transport protein HofC Machinery gene
  BW25113_RS00530 (BW25113_0107) pilB/hofB 112201..113586 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  BW25113_RS00535 (BW25113_0108) pilA/ppdD 113596..114036 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  BW25113_RS00545 (BW25113_0109) nadC 114239..115132 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BW25113_RS00550 (BW25113_0110) ampD 115220..115771 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BW25113_RS00555 (BW25113_0111) ampE 115768..116622 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sxy/tfoX pilC/hofC positive effect
  sxy/tfoX fimT/comN/ppdA positive effect
  sxy/tfoX comE1/ybaV positive effect
  sxy/tfoX pilN/comB/hofN positive effect
  sxy/tfoX pilO/comC/hofO positive effect
  sxy/tfoX pilB/hofB positive effect
  sxy/tfoX pilD/pppA positive effect
  sxy/tfoX rec2/ycaI positive effect
  sxy/tfoX pilP/comD/hofP positive effect
  sxy/tfoX comF/gntX positive effect
  sxy/tfoX pilM/comA/hofM positive effect
  sxy/tfoX pilA/ppdD positive effect
  sxy/tfoX fimU/comO/ppdB positive effect
  sxy/tfoX comP/ygdB positive effect
  sxy/tfoX pilV/comQ/ppdC positive effect
  sxy/tfoX ssb positive effect
  sxy/tfoX mshC/yggT positive effect
  sxy/tfoX pilQ/comE/hofQ positive effect
  sxy/tfoX dprA/smf positive effect
  sxy/tfoX pilT/yggR positive effect
  sxy/tfoX pilB/ycgB positive effect
  sxy/tfoX comM/yifB positive effect

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 44450.49 Da        Isoelectric Point: 8.1770

>NTDB_id=1437 BW25113_RS00525 WP_000157266.1 111009..112211(-) (pilC/hofC) [Escherichia coli BW25113 strain K-12]
MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLSLKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEG
LALLAEQHPSKQWQALLQSLAHDLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSWLLVLFGFLLAIANKLLMRRPTW
LIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSAGITFLQGVESVRETMRCPYWVQLLTQIQHDISNGQPIWLALKNT
GEFSPLCLQLVRTGEASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHLGDAMSGMG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=1437 BW25113_RS00525 WP_000157266.1 111009..112211(-) (pilC/hofC) [Escherichia coli BW25113 strain K-12]
ATGGCGAGTAAGCAACTCTGGCGCTGGCATGGCATAACCGGCGACGGCAATGCGCAAGATGGGATGCTATGGGCAGAGAG
CCGTACTTTACTGCTTATGGCACTACAGCAACAGATGGTTACCCCACTAAGCCTGAAGCGAATCGCCATCAATTCTGCGC
AGTGGCGAGGAGATAAAAGCGCGGAAGTCATTCATCAACTGGCGACGCTACTCAAAGCAGGGTTAACGCTTTCTGAAGGG
CTGGCTCTGCTGGCGGAACAGCATCCCAGTAAGCAATGGCAAGCGTTGCTGCAATCGCTGGCGCACGATCTCGAACAGGG
CATTGCTTTTTCCAATGCCTTATTACCCTGGTCAGAGGTATTTCCGCCGCTCTATCAGGCGATGATCCGCACGGGTGAAC
TGACCGGTAAGCTGGATGAATGCTGCTTTGAACTGGCGCGTCAGCAAAAAGCCCAGCGTCAGTTGACCGACAAAGTGAAA
TCAGCGTTACGTTATCCCATCATCATTTTAGCGATGGCAATCATGGTGGTTGTGGCAATGCTGCATTTTGTTCTGCCGGA
GTTTGCCGCTATCTATAAGACCTTCAACACCCCACTACCGGCACTAACGCAGGGGATCATGACGCTGGCAGACTTTAGTG
GCGAATGGAGCTGGCTGCTGGTGTTGTTCGGCTTTCTGCTGGCGATAGCCAATAAGTTGCTGATGCGCCGACCGACCTGG
CTTATAGTGCGGCAGAAATTGCTGTTACGCATCCCGATTATGGGTTCACTGATGCGGGGACAAAAACTCACGCAGATCTT
TACGATTCTGGCGCTGACACAAAGTGCAGGCATTACTTTTTTACAGGGCGTAGAGAGCGTCAGAGAAACAATGCGCTGCC
CGTACTGGGTGCAACTTCTGACACAAATCCAGCACGATATCAGTAACGGTCAACCCATCTGGCTGGCGCTAAAAAATACC
GGTGAGTTTAGCCCGCTCTGTTTGCAATTAGTGAGAACAGGAGAGGCATCCGGCTCTCTGGATCTCATGTTAGACAACCT
CGCCCATCATCATCGGGAAAACACAATGGCGCTGGCGGATAACCTCGCAGCCTTACTGGAACCGGCGTTGCTGATCATAA
CGGGAGGAATTATTGGTACGCTGGTGGTGGCAATGTATCTGCCAATTTTCCATTTAGGCGATGCGATGAGTGGGATGGGA
TAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(269-388)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A037YRV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value