Detailed information NTG0001433

Bioinformatically predicted

Overview

• Name: pilQ/comE/hofQ
• Type: Machinery gene
• Locus tag: BW25113_RS17570
• Genome accession: NZ_CP009273
• Coordinates: 3512824..3514062 (-)
• Length: 412 a.a.
• NCBI ID: WP_000815987.1
• Uniprot ID: -
• Organism: Escherichia coli BW25113 strain K-12
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3507824..3519062

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BW25113_RS17550 (BW25113_3387)	dam	3508436..3509272 (-)	837	WP_000742143.1	adenine-specific DNA-methyltransferase	-
BW25113_RS17555 (BW25113_3388)	damX	3509379..3510665 (-)	1287	WP_000343215.1	cell division protein DamX	-
BW25113_RS17560 (BW25113_3389)	aroB	3510757..3511845 (-)	1089	WP_000439848.1	3-dehydroquinate synthase	-
BW25113_RS17565 (BW25113_3390)	aroK	3511902..3512423 (-)	522	WP_000818618.1	shikimate kinase AroK	-
BW25113_RS17570 (BW25113_3391)	pilQ/comE/hofQ	3512824..3514062 (-)	1239	WP_000815987.1	DNA uptake porin HofQ	Machinery gene
BW25113_RS17575 (BW25113_3392)	pilP/comD/hofP	3513974..3514378 (-)	405	WP_001264141.1	DNA utilization protein HofP	Machinery gene
BW25113_RS17580 (BW25113_3393)	pilO/comC/hofO	3514368..3514808 (-)	441	WP_001055759.1	DNA utilization protein HofO	Machinery gene
BW25113_RS17585 (BW25113_3394)	pilN/comB/hofN	3514792..3515331 (-)	540	WP_001069315.1	DNA utilization protein HofN	Machinery gene
BW25113_RS17590 (BW25113_3395)	pilM/comA/hofM	3515331..3516110 (-)	780	WP_001295166.1	DNA utilization protein HofM	Machinery gene
BW25113_RS17595 (BW25113_3396)	mrcA	3516230..3518782 (+)	2553	WP_001336003.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
sxy/tfoX	pilQ/comE/hofQ	positive effect
sxy/tfoX	fimT/comN/ppdA	positive effect
sxy/tfoX	comE1/ybaV	positive effect
sxy/tfoX	pilN/comB/hofN	positive effect
sxy/tfoX	pilO/comC/hofO	positive effect
sxy/tfoX	pilB/hofB	positive effect
sxy/tfoX	pilD/pppA	positive effect
sxy/tfoX	rec2/ycaI	positive effect
sxy/tfoX	pilP/comD/hofP	positive effect
sxy/tfoX	comF/gntX	positive effect
sxy/tfoX	pilM/comA/hofM	positive effect
sxy/tfoX	pilA/ppdD	positive effect
sxy/tfoX	fimU/comO/ppdB	positive effect
sxy/tfoX	comP/ygdB	positive effect
sxy/tfoX	pilV/comQ/ppdC	positive effect
sxy/tfoX	ssb	positive effect
sxy/tfoX	mshC/yggT	positive effect
sxy/tfoX	dprA/smf	positive effect
sxy/tfoX	pilT/yggR	positive effect
sxy/tfoX	pilC/hofC	positive effect
sxy/tfoX	pilB/ycgB	positive effect
sxy/tfoX	comM/yifB	positive effect

Sequence

Protein

• Length: 412 a.a.
• Molecular weight: 44716.24 Da
• Isoelectric Point: 6.3183

>NTDB_id=1433 BW25113_RS17570 WP_000815987.1 3512824..3514062(-) (pilQ/comE/hofQ) [Escherichia coli BW25113 strain K-12]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide

• Length: 1239 bp

>NTDB_id=1433 BW25113_RS17570 WP_000815987.1 3512824..3514062(-) (pilQ/comE/hofQ) [Escherichia coli BW25113 strain K-12]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (250-409)

• Bacterial type II/III secretion system short domain (Secretin_N) (120-179)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE	Haemophilus influenzae Rd KW20	37.156	100	0.393
comE	Haemophilus influenzae 86-028NP	36.927	100	0.391
pilQ	Vibrio campbellii strain DS40M4	38.005	100	0.388
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.53	100	0.376
pilQ	Vibrio cholerae strain A1552	37.53	100	0.376
comE	Glaesserella parasuis strain SC1401	35.952	100	0.367