Detailed information NTG0001428

Experimentally validated

Overview

• Name: sxy/tfoX
• Type: Regulator
• Locus tag: BW25113_RS04975
• Genome accession: NZ_CP009273
• Coordinates: 1016594..1017223 (+)
• Length: 209 a.a.
• NCBI ID: WP_000839153.1
• Uniprot ID: A0A9Q6Y251
• Organism: Escherichia coli BW25113 strain K-12
• Function: positive regulator of competence gene Competence regulation

Function

A sxy knockout reduced both natural plasmid transformation and competitive fitness in long-term culture. In addition, expression of plasmid-borne sxy led to production of type IV pilin, the main subunit of the DNA uptake machinery of most bacteria.

Genomic Context

Location: 1011594..1022223

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BW25113_RS04950 (BW25113_0955)	ycbZ	1011995..1013755 (-)	1761	WP_000156518.1	Lon protease family protein	-
BW25113_RS04955 (BW25113_0956)	matP	1013941..1014393 (+)	453	WP_000877161.1	macrodomain Ter protein MatP	-
BW25113_RS04960 (BW25113_0957)	ompA	1014469..1015509 (-)	1041	WP_000750416.1	porin OmpA	-
BW25113_RS04970 (BW25113_0958)	sulA	1015866..1016375 (-)	510	WP_000288710.1	SOS-induced cell division inhibitor SulA	-
BW25113_RS04975 (BW25113_0959)	sxy/tfoX	1016594..1017223 (+)	630	WP_000839153.1	CRP-S regulon transcriptional coactivator Sxy	Regulator
BW25113_RS04980 (BW25113_0960)	yccS	1017186..1019348 (-)	2163	WP_000875061.1	YccS family putative transporter	-
BW25113_RS04985 (BW25113_0961)	yccF	1019358..1019804 (-)	447	WP_001261235.1	YccF domain-containing protein	-
BW25113_RS04990 (BW25113_0962)	helD	1019927..1021981 (+)	2055	WP_001295354.1	DNA helicase IV	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
sxy/tfoX	fimT/comN/ppdA	positive effect
sxy/tfoX	comE1/ybaV	positive effect
sxy/tfoX	pilN/comB/hofN	positive effect
sxy/tfoX	pilO/comC/hofO	positive effect
sxy/tfoX	pilB/hofB	positive effect
sxy/tfoX	pilD/pppA	positive effect
sxy/tfoX	rec2/ycaI	positive effect
sxy/tfoX	pilP/comD/hofP	positive effect
sxy/tfoX	comF/gntX	positive effect
sxy/tfoX	pilM/comA/hofM	positive effect
sxy/tfoX	pilA/ppdD	positive effect
sxy/tfoX	fimU/comO/ppdB	positive effect
sxy/tfoX	comP/ygdB	positive effect
sxy/tfoX	pilV/comQ/ppdC	positive effect
sxy/tfoX	ssb	positive effect
sxy/tfoX	mshC/yggT	positive effect
sxy/tfoX	pilQ/comE/hofQ	positive effect
sxy/tfoX	dprA/smf	positive effect
sxy/tfoX	pilT/yggR	positive effect
sxy/tfoX	pilC/hofC	positive effect
sxy/tfoX	pilB/ycgB	positive effect
sxy/tfoX	comM/yifB	positive effect

Sequence

Protein

• Length: 209 a.a.
• Molecular weight: 24147.02 Da
• Isoelectric Point: 9.2180

>NTDB_id=1428 BW25113_RS04975 WP_000839153.1 1016594..1017223(+) (sxy/tfoX) [Escherichia coli BW25113 strain K-12]
MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVSDGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLN
YYRVDESLWRNQLKLVRLSKYSLDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE

Nucleotide

• Length: 630 bp

>NTDB_id=1428 BW25113_RS04975 WP_000839153.1 1016594..1017223(+) (sxy/tfoX) [Escherichia coli BW25113 strain K-12]
ATGAAAAGCCTCTCCTATAAGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATT
GTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGTATCTTCGGGCTTGTG
AGCAAAGTGCACAGTACTGTGTAAAACATCCGCCTGTCTGGCTGACATATAAAAAGTGTGGCCGATCCGTTACCCTCAAT
TACTATCGGGTTGATGAAAGTCTATGGCGAAATCAACTGAAGCTGGTGCGTCTGTCGAAATATTCTCTCGATGCAGCGCT
GAAAGAGAAAAGCACGCGCAATACCCGGGAAAGACTGAAAGATTTGCCCAATATGTCTTTTCATCTGGAAGCGATTCTCG
GGGAGGTGGGGATTAAGGATGTACGGGCGTTACGTATACTTGGGGCAAAAATGTGTTGGTTGCGACTGCGGCAGCAAAAC
AGTCTGGTGACAGAAAAGATTCTGTTTATGCTTGAAGGTGCCATTATCGGCATTCATGAAGCTGCGCTCCCGGTGGCACG
CCGCCAGGAGCTTGCAGAATGGGCTGACTCTCTTACGCCGAAACAGGAGTTTCCTGCGGAACTTGAGTAA

Domains

Predicted by InterproScan.

• TfoX N-terminal domain (TfoX_N) (14-106)

• TfoX C-terminal domain (TfoX_C) (116-194)

References

• [1]: Sunita Sinha et al. (2009) Sxy induces a CRP-S regulon in Escherichia coli. Journal of Bacteriology 191(16):5180-95. [PMID: 19502395]