Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF/gntX   Type   Machinery gene
Locus tag   BW25113_RS17685 Genome accession   NZ_CP009273
Coordinates   3538241..3538924 (+) Length   227 a.a.
NCBI ID   WP_001333371.1    Uniprot ID   -
Organism   Escherichia coli BW25113 strain K-12     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 3533241..3543924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BW25113_RS17660 (BW25113_3408) feoA 3533522..3533749 (+) 228 WP_001200455.1 ferrous iron transporter A -
  BW25113_RS17665 (BW25113_3409) feoB 3533766..3536087 (+) 2322 WP_000737039.1 Fe(2+) transporter permease subunit FeoB -
  BW25113_RS17670 (BW25113_3410) feoC 3536087..3536323 (+) 237 WP_000157586.1 [Fe-S]-dependent transcriptional repressor FeoC -
  BW25113_RS17675 (BW25113_3411) rpnA 3536526..3537404 (+) 879 WP_000039062.1 recombination-promoting nuclease RpnA -
  BW25113_RS17680 (BW25113_3412) bioH 3537433..3538203 (-) 771 WP_001060070.1 pimeloyl-ACP methyl ester esterase BioH -
  BW25113_RS17685 (BW25113_3413) comF/gntX 3538241..3538924 (+) 684 WP_001333371.1 DNA utilization protein GntX Machinery gene
  BW25113_RS17690 (BW25113_3414) nfuA 3538983..3539558 (+) 576 WP_000619389.1 Fe-S biogenesis protein NfuA -
  BW25113_RS17695 (BW25113_3415) gntT 3539918..3541234 (+) 1317 WP_001131758.1 gluconate transporter -
  BW25113_RS17700 (BW25113_3416) malQ 3541345..3543429 (-) 2085 WP_000444342.1 4-alpha-glucanotransferase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sxy/tfoX comF/gntX positive effect
  sxy/tfoX fimT/comN/ppdA positive effect
  sxy/tfoX comE1/ybaV positive effect
  sxy/tfoX pilN/comB/hofN positive effect
  sxy/tfoX pilO/comC/hofO positive effect
  sxy/tfoX pilB/hofB positive effect
  sxy/tfoX pilD/pppA positive effect
  sxy/tfoX rec2/ycaI positive effect
  sxy/tfoX pilP/comD/hofP positive effect
  sxy/tfoX pilM/comA/hofM positive effect
  sxy/tfoX pilA/ppdD positive effect
  sxy/tfoX fimU/comO/ppdB positive effect
  sxy/tfoX comP/ygdB positive effect
  sxy/tfoX pilV/comQ/ppdC positive effect
  sxy/tfoX ssb positive effect
  sxy/tfoX mshC/yggT positive effect
  sxy/tfoX pilQ/comE/hofQ positive effect
  sxy/tfoX dprA/smf positive effect
  sxy/tfoX pilT/yggR positive effect
  sxy/tfoX pilC/hofC positive effect
  sxy/tfoX pilB/ycgB positive effect
  sxy/tfoX comM/yifB positive effect

Sequence

Protein


Download         Length: 227 a.a.        Molecular weight: 25726.27 Da        Isoelectric Point: 11.0660

>NTDB_id=1434 BW25113_RS17685 WP_001333371.1 3538241..3538924(+) (comF/gntX) [Escherichia coli BW25113 strain K-12]
MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPATHSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQ
LKFSRRSEIASALSRLLLLEVLHARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAVQVWCLCRTL

Nucleotide


Download         Length: 684 bp        

>NTDB_id=1434 BW25113_RS17685 WP_001333371.1 3538241..3538924(+) (comF/gntX) [Escherichia coli BW25113 strain K-12]
ATGCTAACAGTACCGGGATTATGCTGGCTATGCCGAATGCCACTGGCGTTAGGTCATTGGGGGATTTGTTCGGTCTGTTC
ACGCGCCACCCGCACAGATAAAACGTTATGCCCACAATGTGGATTACCCGCCACACACTCCCATCTTCCCTGCGGTCGCT
GCCTGCAAAAACCGCCGCCCTGGCAAAGACTGGTCACGGTTGCCGACTATGCGCCGCCGTTAAGTCCGCTTATCCACCAG
CTTAAATTTTCCCGGCGCAGTGAAATCGCCAGCGCCCTGTCACGTCTGTTACTATTGGAAGTCTTACACGCTCGTCGCAC
CACCGGGTTGCAATTGCCGGATCGCATCGTCAGCGTTCCGTTATGGCAGCGGCGTCACTGGCGTCGGGGATTTAATCAGA
GCGATTTGCTGTGTCAGCCGTTATCACGCTGGTTGCACTGCCAATGGGATAGCGAAGCCGTCACACGTACACGGGCCACT
GCGACCCAGCATTTTCTCAGTGCCCGGCTGCGCAAGCGCAACCTGAAAAATGCCTTTCGTCTTGAATTGCCCGTGCAAGG
TCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATG
GTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAG

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(169-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Haemophilus influenzae 86-028NP

43.75

98.678

0.432
  comF Haemophilus influenzae Rd KW20

43.304

98.678

0.427
  comF Vibrio cholerae strain A1552

41.818

96.916

0.405
  comF Vibrio campbellii strain DS40M4

36.245

100

0.366

Multiple sequence alignment