Detailed information NTG0001441

Bioinformatically predicted

Overview

• Name: fimT/comN/ppdA
• Type: Machinery gene
• Locus tag: BW25113_RS14695
• Genome accession: NZ_CP009273
• Coordinates: 2957066..2957536 (-)
• Length: 156 a.a.
• NCBI ID: WP_000857051.1
• Uniprot ID: A0AA35F7L2
• Organism: Escherichia coli BW25113 strain K-12
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2952066..2962536

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BW25113_RS14675 (BW25113_2822)	recC	2952419..2955787 (-)	3369	WP_000946938.1	exodeoxyribonuclease V subunit gamma	-
BW25113_RS14680 (BW25113_2823)	pilV/comQ/ppdC	2955800..2956123 (-)	324	WP_001276460.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
BW25113_RS14685 (BW25113_2824)	comP/ygdB	2956108..2956515 (-)	408	WP_001078376.1	DUF2509 family protein	Machinery gene
BW25113_RS14690 (BW25113_2825)	fimU/comO/ppdB	2956512..2957075 (-)	564	WP_001144315.1	prepilin peptidase-dependent protein	Machinery gene
BW25113_RS14695 (BW25113_2826)	fimT/comN/ppdA	2957066..2957536 (-)	471	WP_000857051.1	prepilin peptidase-dependent protein	Machinery gene
BW25113_RS14700 (BW25113_2827)	thyA	2957720..2958514 (-)	795	WP_000816232.1	thymidylate synthase	-
BW25113_RS14705 (BW25113_2828)	lgt	2958521..2959396 (-)	876	WP_000204658.1	prolipoprotein diacylglyceryl transferase	-
BW25113_RS14710 (BW25113_2829)	ptsP	2959547..2961793 (-)	2247	WP_000957910.1	phosphoenolpyruvate--protein phosphotransferase	-
BW25113_RS14715 (BW25113_2830)	rppH	2961806..2962336 (-)	531	WP_000564489.1	RNA pyrophosphohydrolase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
sxy/tfoX	fimT/comN/ppdA	positive effect
sxy/tfoX	comE1/ybaV	positive effect
sxy/tfoX	pilN/comB/hofN	positive effect
sxy/tfoX	pilO/comC/hofO	positive effect
sxy/tfoX	pilB/hofB	positive effect
sxy/tfoX	pilD/pppA	positive effect
sxy/tfoX	rec2/ycaI	positive effect
sxy/tfoX	pilP/comD/hofP	positive effect
sxy/tfoX	comF/gntX	positive effect
sxy/tfoX	pilM/comA/hofM	positive effect
sxy/tfoX	pilA/ppdD	positive effect
sxy/tfoX	fimU/comO/ppdB	positive effect
sxy/tfoX	comP/ygdB	positive effect
sxy/tfoX	pilV/comQ/ppdC	positive effect
sxy/tfoX	ssb	positive effect
sxy/tfoX	mshC/yggT	positive effect
sxy/tfoX	pilQ/comE/hofQ	positive effect
sxy/tfoX	dprA/smf	positive effect
sxy/tfoX	pilT/yggR	positive effect
sxy/tfoX	pilC/hofC	positive effect
sxy/tfoX	pilB/ycgB	positive effect
sxy/tfoX	comM/yifB	positive effect

Sequence

Protein

• Length: 156 a.a.
• Molecular weight: 17865.41 Da
• Isoelectric Point: 7.7262

>NTDB_id=1441 BW25113_RS14695 WP_000857051.1 2957066..2957536(-) (fimT/comN/ppdA) [Escherichia coli BW25113 strain K-12]
MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTASQARDYLLYLREDANWHNRDHSISVIREGTLWCLVSS
AAGANTCHGSSPLVFVPRWPEVEMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL

Nucleotide

• Length: 471 bp

>NTDB_id=1441 BW25113_RS14695 WP_000857051.1 2957066..2957536(-) (fimT/comN/ppdA) [Escherichia coli BW25113 strain K-12]
ATGAAAACACAACGTGGTTATACGCTGATTGAAACGCTGGTCGCGATGCTGATTTTGGTCATGCTAAGCGCAAGTGGACT
CTATGGCTGGCAATACTGGCAGCAGTCGCAACGGCTATGGCAAACCGCCAGCCAGGCGCGGGACTATTTGCTCTATTTAC
GTGAAGATGCCAACTGGCATAACCGCGACCACAGTATCAGCGTTATCAGGGAGGGGACGTTATGGTGCCTTGTGAGTTCC
GCTGCTGGGGCCAATACCTGTCATGGCAGTTCACCATTGGTCTTTGTGCCACGCTGGCCCGAAGTCGAAATGAGCGACCT
GACACCTTCGCTTGCTTTCTTTGGCCTGCGCAATACCGCATGGGCCGGGCATATTCGCTTCAAAAACTCAACGGGCGAGT
GGTGGCTGGTGGTTTCGCCGTGGGGAAGACTCCGGCTTTGTCAGCAAGGAGAAACAGAAGGATGCCTGTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-25)