Detailed information NTG0001430

Bioinformatically predicted

Overview

• Name: pilN/comB/hofN
• Type: Machinery gene
• Locus tag: BW25113_RS17585
• Genome accession: NZ_CP009273
• Coordinates: 3514792..3515331 (-)
• Length: 179 a.a.
• NCBI ID: WP_001069315.1
• Uniprot ID: W8TUB8
• Organism: Escherichia coli BW25113 strain K-12
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3509792..3520331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BW25113_RS17560 (BW25113_3389)	aroB	3510757..3511845 (-)	1089	WP_000439848.1	3-dehydroquinate synthase	-
BW25113_RS17565 (BW25113_3390)	aroK	3511902..3512423 (-)	522	WP_000818618.1	shikimate kinase AroK	-
BW25113_RS17570 (BW25113_3391)	pilQ/comE/hofQ	3512824..3514062 (-)	1239	WP_000815987.1	DNA uptake porin HofQ	Machinery gene
BW25113_RS17575 (BW25113_3392)	pilP/comD/hofP	3513974..3514378 (-)	405	WP_001264141.1	DNA utilization protein HofP	Machinery gene
BW25113_RS17580 (BW25113_3393)	pilO/comC/hofO	3514368..3514808 (-)	441	WP_001055759.1	DNA utilization protein HofO	Machinery gene
BW25113_RS17585 (BW25113_3394)	pilN/comB/hofN	3514792..3515331 (-)	540	WP_001069315.1	DNA utilization protein HofN	Machinery gene
BW25113_RS17590 (BW25113_3395)	pilM/comA/hofM	3515331..3516110 (-)	780	WP_001295166.1	DNA utilization protein HofM	Machinery gene
BW25113_RS17595 (BW25113_3396)	mrcA	3516230..3518782 (+)	2553	WP_001336003.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-
BW25113_RS17600 (BW25113_3397)	nudE	3518948..3519508 (-)	561	WP_000045744.1	ADP compounds hydrolase NudE	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
sxy/tfoX	pilN/comB/hofN	positive effect
sxy/tfoX	fimT/comN/ppdA	positive effect
sxy/tfoX	comE1/ybaV	positive effect
sxy/tfoX	pilO/comC/hofO	positive effect
sxy/tfoX	pilB/hofB	positive effect
sxy/tfoX	pilD/pppA	positive effect
sxy/tfoX	rec2/ycaI	positive effect
sxy/tfoX	pilP/comD/hofP	positive effect
sxy/tfoX	comF/gntX	positive effect
sxy/tfoX	pilM/comA/hofM	positive effect
sxy/tfoX	pilA/ppdD	positive effect
sxy/tfoX	fimU/comO/ppdB	positive effect
sxy/tfoX	comP/ygdB	positive effect
sxy/tfoX	pilV/comQ/ppdC	positive effect
sxy/tfoX	ssb	positive effect
sxy/tfoX	mshC/yggT	positive effect
sxy/tfoX	pilQ/comE/hofQ	positive effect
sxy/tfoX	dprA/smf	positive effect
sxy/tfoX	pilT/yggR	positive effect
sxy/tfoX	pilC/hofC	positive effect
sxy/tfoX	pilB/ycgB	positive effect
sxy/tfoX	comM/yifB	positive effect

Sequence

Protein

• Length: 179 a.a.
• Molecular weight: 20795.89 Da
• Isoelectric Point: 11.4775

>NTDB_id=1430 BW25113_RS17585 WP_001069315.1 3514792..3515331(-) (pilN/comB/hofN) [Escherichia coli BW25113 strain K-12]
MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTGSAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRE
QRSQRQRQRQFTRDWQSALEALAALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
GRWQFEYQLTRKVSDEHVL

Nucleotide

• Length: 540 bp

>NTDB_id=1430 BW25113_RS17585 WP_001069315.1 3514792..3515331(-) (pilN/comB/hofN) [Escherichia coli BW25113 strain K-12]
ATGAACCCGCCAATTAATTTTTTGCCCTGGCGACAGCAACGCCGGACCGCTTTTCTGCGTTTCTGGTTGCTGATGTTCGT
TGCGCCTCTGCTGCTGGCCGTCGGGATAACGCTAATACTGCGTCTGACAGGCAGCGCCGAAGCGCGCATAGACGCAGTTT
TGCTTCAGGCGGAACAACAACTCGCCCGCAGCTTACAGATAACGAAGCCACGTTTGCTGGAGCAACAGCAGTTACGCGAA
CAGCGTTCGCAACGGCAGCGCCAGCGACAATTTACCCGCGACTGGCAATCTGCGCTGGAAGCACTGGCGGCGCTTTTACC
CGAGCACGCCTGGCTGACAACGATAAGCTGGCAGCAGGGAACGCTGGAGATCAAGGGGCTGACAACAAGCATTACCGCGT
TAAACGCACTAGAAACGTCACTCCGCCAGGATGCATCTTTTCATCTCAATCAGCGGGGAGCCACGCAGCAGGATGCGCAG
GGACGCTGGCAATTTGAGTATCAGTTAACAAGGAAGGTTAGCGATGAACATGTTCTTTGA

Domains

Predicted by InterproScan.

• Fimbrial assembly protein (PilN) (PilN) (99-169)

3D structure

• AlphaFold DB: W8TUB8