Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F384_RS25875 Genome accession   NZ_CP011132
Coordinates   5532906..5533886 (+) Length   326 a.a.
NCBI ID   WP_052746991.1    Uniprot ID   -
Organism   Citrobacter amalonaticus Y19     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5527906..5538886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F384_RS25845 (F384_25840) - 5528542..5529549 (+) 1008 WP_046497228.1 DUF1202 family protein -
  F384_RS25850 (F384_25845) hemW 5529582..5530718 (-) 1137 WP_046497230.1 radical SAM family heme chaperone HemW -
  F384_RS25855 (F384_25850) - 5530711..5531304 (-) 594 WP_046497233.1 XTP/dITP diphosphatase -
  F384_RS25860 (F384_25855) yggU 5531312..5531602 (-) 291 WP_044255664.1 DUF167 family protein YggU -
  F384_RS25865 (F384_25860) - 5531599..5532165 (-) 567 WP_046497238.1 YggT family protein -
  F384_RS25870 (F384_25865) - 5532184..5532888 (-) 705 WP_046497242.1 YggS family pyridoxal phosphate-dependent enzyme -
  F384_RS25875 (F384_25870) pilT 5532906..5533886 (+) 981 WP_052746991.1 type IV pilus twitching motility protein PilT Machinery gene
  F384_RS25880 (F384_25875) ruvX 5533900..5534316 (-) 417 WP_046497251.1 Holliday junction resolvase RuvX -
  F384_RS25885 (F384_25880) - 5534316..5534879 (-) 564 WP_046497256.1 YqgE/AlgH family protein -
  F384_RS30070 - 5535404..5535682 (+) 279 WP_155404035.1 hypothetical protein -
  F384_RS25890 (F384_25885) gshB 5535719..5536666 (-) 948 WP_046497260.1 glutathione synthase -
  F384_RS25895 (F384_25890) rsmE 5536679..5537410 (-) 732 WP_046497263.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F384_RS25900 (F384_25895) endA 5537490..5538197 (-) 708 WP_046497264.1 deoxyribonuclease I -
  F384_RS25905 (F384_25900) - 5538289..5538789 (-) 501 WP_052746992.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35748.88 Da        Isoelectric Point: 6.8264

>NTDB_id=142664 F384_RS25875 WP_052746991.1 5532906..5533886(+) (pilT) [Citrobacter amalonaticus Y19]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGHMECAPFTSADITGLLADWLDEGQQAALQKNGQLDFAVTLVGNLRLRA
SAFQQRQGTSLALRLLPTQCPTLERLETPSVLPELLRSENGLILVTGATGSGKSTTLAAMVAWLNQHVDGHILTLEDPIE
YVYTSQRCLIQQREVGLHCTSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDT
FPAQEKDPVRSQLAGSLRAVLSQKLESDKQEGRVALFELLINTPAAGNLIREGKSHQLPHVIQTGQQVGMMTFSQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=142664 F384_RS25875 WP_052746991.1 5532906..5533886(+) (pilT) [Citrobacter amalonaticus Y19]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCACG
CTGGCGAAAACGGGGGCACATGGAATGCGCGCCCTTCACCTCCGCCGACATTACCGGACTGCTGGCGGACTGGCTGGATG
AAGGGCAACAGGCGGCGTTACAGAAAAACGGGCAACTGGATTTCGCCGTAACGCTTGTGGGAAACCTGCGGTTGCGGGCC
AGTGCATTTCAGCAGCGGCAGGGAACGTCGCTGGCGCTGCGACTGTTGCCGACGCAATGCCCAACGCTGGAAAGGCTGGA
GACACCCTCCGTGCTGCCGGAACTGCTTCGCAGCGAGAACGGTTTGATTCTGGTGACGGGGGCGACGGGCAGCGGGAAAT
CAACCACCCTGGCGGCGATGGTGGCGTGGCTGAATCAGCATGTCGATGGGCACATTCTGACGCTGGAAGATCCCATAGAG
TATGTCTACACCAGCCAGCGCTGTCTGATCCAACAGCGTGAAGTGGGATTACACTGCACTTCGTTTGCCGCGGGGTTGCG
CGCGGCGCTGCGTGAAGATCCGGATGTGATCCTGCTGGGGGAGTTACGTGACAGCGAAACCATTCGTCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACACTGCATACGCGCGGAGCCGCGCAGGCGATCGAGCGGTTGATCGACACT
TTCCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCCGGTAGCCTGCGCGCCGTGCTGTCGCAAAAACTGGAGTC
CGACAAGCAGGAGGGGCGCGTTGCGTTATTTGAACTGCTGATTAACACCCCGGCGGCGGGGAATTTGATTCGCGAAGGGA
AGTCCCACCAGTTGCCCCACGTCATTCAGACCGGGCAGCAGGTGGGGATGATGACCTTTTCCCAGAGTCTGCAACAGCGA
CAGGCGCAGGGACGGCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.925

98.773

0.414
  pilU Vibrio cholerae strain A1552

43.226

95.092

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

38.769

99.693

0.387

Multiple sequence alignment