Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VO64_RS14095 Genome accession   NZ_CP011117
Coordinates   2961392..2962426 (-) Length   344 a.a.
NCBI ID   WP_046069934.1    Uniprot ID   A0AAU8TLK7
Organism   Pseudomonas synxantha strain LBUM223     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2956392..2967426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VO64_RS14065 (VO64_2642) ruvX 2956504..2956941 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  VO64_RS14070 (VO64_2643) pyrR 2957075..2957581 (+) 507 WP_046069929.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  VO64_RS14075 (VO64_2644) - 2957606..2958610 (+) 1005 WP_046069930.1 aspartate carbamoyltransferase catalytic subunit -
  VO64_RS14080 (VO64_2645) - 2958607..2959878 (+) 1272 WP_046069931.1 dihydroorotase -
  VO64_RS14085 (VO64_2646) - 2959998..2960426 (-) 429 WP_046069932.1 TM2 domain-containing protein -
  VO64_RS14090 (VO64_2647) - 2960676..2961314 (+) 639 WP_046069933.1 C40 family peptidase -
  VO64_RS14095 (VO64_2648) pilT 2961392..2962426 (-) 1035 WP_046069934.1 type IV pilus twitching motility protein PilT Machinery gene
  VO64_RS14100 (VO64_2649) - 2962484..2963170 (+) 687 WP_046069935.1 YggS family pyridoxal phosphate-dependent enzyme -
  VO64_RS14105 (VO64_2650) proC 2963205..2964023 (+) 819 WP_046069936.1 pyrroline-5-carboxylate reductase -
  VO64_RS14110 (VO64_2651) - 2964049..2964636 (+) 588 WP_046069937.1 YggT family protein -
  VO64_RS14115 (VO64_2652) metX 2964778..2965920 (+) 1143 WP_046069938.1 homoserine O-succinyltransferase MetX -
  VO64_RS14120 (VO64_2653) metW 2965946..2966566 (+) 621 WP_046069939.1 methionine biosynthesis protein MetW -
  VO64_RS14125 (VO64_2654) - 2966588..2967025 (+) 438 WP_046069940.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37655.54 Da        Isoelectric Point: 7.3204

>NTDB_id=142496 VO64_RS14095 WP_046069934.1 2961392..2962426(-) (pilT) [Pseudomonas synxantha strain LBUM223]
MDITQLLTASVSRGASDLHLSTGLVPMLRIDGEVWPMDWPVLEASQMVDLVCPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQDRGVGAVFRTIPTEVQSLEDLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLED
PIEFIHRPKKSLITQRQVHRDTHSFSVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
IDVFPAGEKVMVRSMLSESLQAVVSQVLVKKVGGGRVAAHELMLGTPAIRNLIREDKVAQVVSAIQTGGALGMKTLDMSL
KALVARGVVSRDDAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=142496 VO64_RS14095 WP_046069934.1 2961392..2962426(-) (pilT) [Pseudomonas synxantha strain LBUM223]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGAGGCGCCTCCGACCTGCATCTGTCGACCGGCCTGGTGCCGAT
GCTGCGCATCGATGGCGAGGTCTGGCCCATGGATTGGCCGGTGCTTGAGGCATCGCAAATGGTCGACCTGGTGTGCCCGT
TGCTGAATAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAGTTGCCCGGGGTGGCGCGT
TTTCGAGCCAACGTGTTCCGCCAGGACCGCGGCGTGGGTGCGGTATTTCGCACTATCCCGACCGAGGTCCAGAGCCTGGA
AGACCTGGGGCTGGGCGAGGTTTTCCAGCGTATCGCCCAGCTTCCACGCGGCCTGGTATTGGTCACTGGGCCCACCGGCT
CGGGTAAGTCCACCACCTTGGCCGCGATGATCGACCACCTTAACCAGCATCGACGCCAGCATATCCTCACCCTGGAAGAT
CCTATTGAATTTATCCACAGGCCGAAAAAGTCCCTGATCACCCAGCGCCAAGTGCACCGCGATACCCACAGCTTTTCGGT
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGATGTGATACTGGTGGGTGAGCTACGCGACCTGGAAACCATTCGCCTGG
CGCTGACGGCCGCTGAGACCGGGCACCTGGTATTTGGCACCCTCCACACTTCATCGGCAGCAAAGACCGTGGACAGGCTG
ATAGACGTCTTCCCTGCCGGGGAAAAGGTCATGGTTCGCTCAATGCTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAAGT
GCTGGTGAAGAAGGTCGGCGGCGGGCGCGTGGCGGCTCATGAACTCATGCTGGGCACGCCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAGGTGGTCTCGGCGATTCAGACCGGTGGGGCGTTGGGGATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTTGCGCGAGGCGTGGTCAGTCGAGACGATGCGCGAGAGAAGGCGAGGGTGCCTGCAGACATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

70.93

100

0.709
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Acinetobacter baumannii D1279779

69.795

99.128

0.692
  pilT Acinetobacter baumannii strain A118

69.795

99.128

0.692
  pilT Acinetobacter nosocomialis M2

69.501

99.128

0.689
  pilT Acinetobacter baylyi ADP1

68.035

99.128

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.965

0.64
  pilT Vibrio cholerae strain A1552

65.282

97.965

0.64
  pilT Legionella pneumophila strain Lp02

63.018

98.256

0.619
  pilT Legionella pneumophila strain ERS1305867

63.018

98.256

0.619
  pilT Neisseria meningitidis 8013

60.882

98.837

0.602
  pilT Neisseria gonorrhoeae MS11

60.588

98.837

0.599
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Vibrio cholerae strain A1552

39.13

100

0.392
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39

Multiple sequence alignment