Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JBW_RS11035 Genome accession   NZ_CP010978
Coordinates   2517497..2518546 (+) Length   349 a.a.
NCBI ID   WP_007956618.1    Uniprot ID   I8TVV4
Organism   Pelosinus fermentans JBW45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2512497..2523546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JBW_RS11015 (JBW_02225) - 2512918..2513763 (+) 846 WP_007956621.1 shikimate dehydrogenase -
  JBW_RS11020 (JBW_02226) - 2513958..2514521 (+) 564 WP_007956620.1 hypothetical protein -
  JBW_RS11025 (JBW_02227) - 2514514..2515734 (+) 1221 WP_007934435.1 type II secretion system protein GspD -
  JBW_RS11030 (JBW_02228) - 2515956..2517497 (+) 1542 WP_007956619.1 GspE/PulE family protein -
  JBW_RS11035 (JBW_02229) pilT 2517497..2518546 (+) 1050 WP_007956618.1 type IV pilus twitching motility protein PilT Machinery gene
  JBW_RS11040 (JBW_02230) - 2518572..2519792 (+) 1221 WP_007956617.1 type II secretion system F family protein -
  JBW_RS11045 (JBW_02231) - 2519842..2520240 (+) 399 WP_007956616.1 type II secretion system protein -
  JBW_RS11050 (JBW_02232) - 2520306..2521046 (+) 741 WP_007956615.1 prepilin peptidase -
  JBW_RS11055 (JBW_02233) - 2521056..2521517 (+) 462 WP_007956614.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JBW_RS11060 (JBW_02234) - 2521528..2521932 (+) 405 WP_007956613.1 hypothetical protein -
  JBW_RS11065 (JBW_02235) - 2521960..2522469 (+) 510 WP_007956612.1 PilW family protein -
  JBW_RS11070 (JBW_02236) - 2522509..2522940 (+) 432 WP_235334189.1 pilus assembly PilX N-terminal domain-containing protein -
  JBW_RS24050 (JBW_02237) - 2522955..2523131 (+) 177 WP_007956610.1 late competence development ComFB family protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38905.65 Da        Isoelectric Point: 7.3671

>NTDB_id=141051 JBW_RS11035 WP_007956618.1 2517497..2518546(+) (pilT) [Pelosinus fermentans JBW45]
MDQLLDQAVHSRASDLHITVGAPPIFRIDGQLVPTNHQLLNVKNTERLFKAITTPQQQQYFNEHGEIDFSFAVPGLSRFR
VNAFHQRGSVAIVIRVIDENIPTLEGLGHPRVIRTLACQARGLVLVTGPTGSGKSTTLAAMINLINQERFCHILTLEDPI
EFLHKHNKSIINQREINTDTKSFTVALRAALREDPDVILVGEMRDSETIATAITAAETGHLVFATLHTSSAVQTIDRIID
AFPSHQQQQIRIQLSVTLQGIIAQQLLPRMDQIGRVSALEILVATPAVRNMIREGKTHQLTSFIQTGAKFGMQTMDIALR
DLYRRGIISYGEAIMRVIDEENFARFANN

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=141051 JBW_RS11035 WP_007956618.1 2517497..2518546(+) (pilT) [Pelosinus fermentans JBW45]
ATGGATCAGCTATTGGATCAAGCTGTACATAGCCGAGCATCTGACCTGCACATTACAGTAGGCGCACCACCCATATTCAG
AATTGATGGTCAACTTGTACCTACTAACCATCAGCTACTAAACGTAAAAAATACAGAAAGATTGTTTAAAGCCATTACAA
CGCCGCAGCAACAGCAATATTTTAATGAGCATGGTGAAATTGACTTTTCCTTTGCAGTTCCTGGCCTTAGCCGTTTTCGA
GTCAATGCTTTTCACCAACGTGGTTCAGTGGCTATAGTGATTCGCGTTATAGATGAAAATATACCTACATTAGAAGGGCT
GGGACATCCACGTGTTATAAGAACTCTTGCTTGTCAGGCTAGAGGTTTAGTTCTAGTTACCGGTCCCACAGGTAGTGGCA
AATCAACAACTTTGGCGGCGATGATTAATCTGATTAACCAGGAACGATTCTGTCATATTCTTACACTAGAAGATCCTATT
GAATTTTTACATAAGCATAACAAGTCAATTATCAATCAGCGGGAAATCAATACTGATACCAAATCGTTTACGGTTGCTCT
CAGAGCTGCTTTAAGAGAAGATCCAGATGTTATTTTGGTAGGAGAAATGCGTGATTCGGAAACCATTGCTACAGCCATTA
CGGCAGCAGAAACAGGACACTTAGTCTTTGCTACTTTACACACTAGCAGTGCTGTTCAAACTATAGATCGCATTATTGAT
GCTTTTCCTTCACATCAACAGCAGCAAATTCGTATACAGCTGTCCGTTACCCTGCAAGGAATTATTGCTCAGCAGTTATT
ACCTCGAATGGATCAGATTGGCAGAGTATCTGCTTTAGAAATTCTGGTAGCTACTCCTGCAGTGCGTAACATGATTCGTG
AAGGAAAGACCCATCAACTGACTTCCTTCATTCAAACGGGCGCAAAGTTTGGTATGCAAACGATGGACATTGCTTTACGG
GATTTATATCGTCGTGGAATCATCTCGTATGGTGAAGCTATAATGCGCGTCATAGATGAAGAGAACTTCGCTCGTTTCGC
CAATAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(4-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I8TVV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.624

99.14

0.542
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

94.556

0.513
  pilT Vibrio cholerae strain A1552

54.242

94.556

0.513
  pilT Acinetobacter baylyi ADP1

52.199

97.708

0.51
  pilT Pseudomonas stutzeri DSM 10701

51.32

97.708

0.501
  pilT Legionella pneumophila strain ERS1305867

53.211

93.696

0.499
  pilT Legionella pneumophila strain Lp02

53.211

93.696

0.499
  pilT Pseudomonas aeruginosa PAK

51.026

97.708

0.499
  pilT Acinetobacter baumannii strain A118

52.905

93.696

0.496
  pilT Acinetobacter baumannii D1279779

52.905

93.696

0.496
  pilT Acinetobacter nosocomialis M2

52.905

93.696

0.496
  pilT Neisseria meningitidis 8013

49.56

97.708

0.484
  pilT Neisseria gonorrhoeae MS11

49.267

97.708

0.481
  pilU Pseudomonas stutzeri DSM 10701

44.058

98.854

0.436
  pilU Vibrio cholerae strain A1552

45.21

95.702

0.433
  pilU Acinetobacter baylyi ADP1

43.413

95.702

0.415

Multiple sequence alignment