Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B723_RS02955 Genome accession   NZ_CP010945
Coordinates   639270..640304 (-) Length   344 a.a.
NCBI ID   WP_017341273.1    Uniprot ID   A0A0K1QI06
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 634270..645304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS02925 (B723_02930) ruvX 634502..634939 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  B723_RS02930 (B723_02935) pyrR 635023..635526 (+) 504 WP_008026190.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  B723_RS02935 (B723_02940) - 635554..636558 (+) 1005 WP_017341269.1 aspartate carbamoyltransferase catalytic subunit -
  B723_RS02940 (B723_02945) - 636555..637826 (+) 1272 WP_017341270.1 dihydroorotase -
  B723_RS02945 (B723_02950) - 637921..638352 (-) 432 WP_017341271.1 TM2 domain-containing protein -
  B723_RS02950 (B723_02955) - 638602..639219 (+) 618 WP_017341272.1 C40 family peptidase -
  B723_RS02955 (B723_02960) pilT 639270..640304 (-) 1035 WP_017341273.1 type IV pilus twitching motility protein PilT Machinery gene
  B723_RS02960 (B723_02965) - 640361..641047 (+) 687 WP_017341274.1 YggS family pyridoxal phosphate-dependent enzyme -
  B723_RS02965 (B723_02970) proC 641083..641901 (+) 819 WP_017341275.1 pyrroline-5-carboxylate reductase -
  B723_RS02970 (B723_02975) - 641912..642502 (+) 591 WP_017341276.1 YggT family protein -
  B723_RS02975 (B723_02980) - 642507..642803 (+) 297 WP_017341277.1 DUF167 domain-containing protein -
  B723_RS02980 (B723_02985) - 642931..644070 (+) 1140 WP_017341278.1 homoserine O-acetyltransferase -
  B723_RS02985 (B723_02990) metW 644078..644698 (+) 621 WP_017341279.1 methionine biosynthesis protein MetW -
  B723_RS02990 (B723_02995) - 644725..645159 (+) 435 WP_017341280.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38148.78 Da        Isoelectric Point: 6.9102

>NTDB_id=140795 B723_RS02955 WP_017341273.1 639270..640304(-) (pilT) [Pseudomonas fluorescens NCIMB 11764]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKEVHELIYDIMNDLQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEVFRKITEAPRGLVLVTGPTGSGKSTTLAAMIDHLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KDLVTKGFISREHAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=140795 B723_RS02955 WP_017341273.1 639270..640304(-) (pilT) [Pseudomonas fluorescens NCIMB 11764]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAAGGCGCGTCCGACTTGCACCTGTCGGCAGGCTTGCCCCCGAT
GATTCGCGTCGACGGCGATGTGCGGCGCATCAACCTGCCGGCTCTGGACCACAAGGAGGTGCACGAGCTGATCTACGACA
TCATGAACGACCTGCAGCGGGTGGACTTCGAGAAGCATCTGGAAACCGACTTCTCATTCGAAGTCCCCGGCGTGGCGCGT
TTTCGGGTCAATGCCTTCAACCAGAACCGTGGTGCAGGCGCCGTATTCCGGACCATTCCGTCCAAGGTCCTGAGCATGGA
GGACCTGGGCATGGGGGAAGTGTTTCGCAAGATTACCGAAGCGCCCCGAGGCCTGGTGCTGGTGACCGGACCGACCGGTT
CCGGCAAGTCCACCACTTTGGCGGCGATGATCGATCACCTGAACAACCATCGCCATCACCACATTCTGACCATCGAAGAC
CCGATCGAATTTGTCCACGAGTCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCATCGCGATACCCGCAGTTTCGCCAC
GGCGTTGCGTTCGGCGCTGCGGGAAGACCCGGATGTGATTCTGGTGGGGGAGATGCGGGATCTGGAGACCATTCGTCTGG
CGCTGACCGCTGCCGAGACCGGTCACCTGGTGTTCGGCACGCTGCACACCACGTCGGCGGCGAAAACCATCGACCGGGTA
GTGGATGTGTTTCCGGGGGACGAGAAGTCGATGGTGCGTTCGATGCTGTCCGAATCATTGCTGGCGGTGGTGTCGCAAAC
GCTGGTGAAGAAGATCGGCGGGGGCCGCATCGCGGCGCACGAGATCATGCTGGGGACATCGGCGATCCGTAACCTGATCC
GCGAGGACAAAGTGGCGCAGATGTACTCGTCGATTCAGACCGGCGGGAATCTGGGGATGCAGACGCTGGATATGTGCTTG
AAGGATCTGGTGACCAAAGGCTTTATCAGTCGCGAGCACGCTCGGGAGAAGGCGCGGTCGCCGGATAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1QI06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Pseudomonas aeruginosa PAK

86.337

100

0.863
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

71.512

100

0.715
  pilT Legionella pneumophila strain ERS1305867

71.512

100

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Vibrio cholerae strain A1552

40

100

0.401

Multiple sequence alignment