Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TO66_RS29530 Genome accession   NZ_CP010892
Coordinates   6470504..6471538 (-) Length   344 a.a.
NCBI ID   WP_044465568.1    Uniprot ID   A0A0C5E906
Organism   Pseudomonas sp. MRSN 12121 strain MRSN12121     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6465504..6476538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO66_RS29500 (TO66_29490) ruvX 6465661..6466098 (+) 438 WP_044465562.1 Holliday junction resolvase RuvX -
  TO66_RS29505 (TO66_29495) pyrR 6466220..6466726 (+) 507 WP_044465563.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  TO66_RS29510 (TO66_29500) - 6466752..6467756 (+) 1005 WP_044465564.1 aspartate carbamoyltransferase catalytic subunit -
  TO66_RS29515 (TO66_29505) - 6467753..6469024 (+) 1272 WP_044465565.1 dihydroorotase -
  TO66_RS29520 (TO66_29510) - 6469134..6469553 (-) 420 WP_044465566.1 TM2 domain-containing protein -
  TO66_RS29525 (TO66_29515) - 6469804..6470439 (+) 636 WP_044465567.1 C40 family peptidase -
  TO66_RS29530 (TO66_29520) pilT 6470504..6471538 (-) 1035 WP_044465568.1 type IV pilus twitching motility protein PilT Machinery gene
  TO66_RS29535 (TO66_29525) - 6471596..6472294 (+) 699 WP_044465569.1 YggS family pyridoxal phosphate-dependent enzyme -
  TO66_RS29540 (TO66_29530) proC 6472329..6473147 (+) 819 WP_044465570.1 pyrroline-5-carboxylate reductase -
  TO66_RS29545 (TO66_29535) - 6473158..6473748 (+) 591 WP_044465571.1 YggT family protein -
  TO66_RS29550 (TO66_29540) - 6473762..6474058 (+) 297 WP_044465572.1 DUF167 domain-containing protein -
  TO66_RS29555 (TO66_29545) - 6474254..6475393 (+) 1140 WP_044465573.1 homoserine O-acetyltransferase -
  TO66_RS29560 (TO66_29550) metW 6475404..6476024 (+) 621 WP_023967992.1 methionine biosynthesis protein MetW -
  TO66_RS29565 (TO66_29555) - 6476050..6476484 (+) 435 WP_044465574.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37398.06 Da        Isoelectric Point: 6.8741

>NTDB_id=140681 TO66_RS29530 WP_044465568.1 6470504..6471538(-) (pilT) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
MDITELLAFSVKQGASDLHLSAGLPPMIRIDGDIRRINLPLLEAPQVHELILASMTDPQRAEFKAALEIDYSFALPGVAR
FRVNAFNHQRGNGAVFRSIPDQVPSLDALGLGEVFRRISEAPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRHQHILTIED
PIEFIHEPKKCLIHQREVQRDTHSFSSALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTTSAAKTVDRV
VDVFPAEEKAMVRSVLSESLQAVISQALLKRVGGGRVAAHEIMLGTAAIRNLIREDKVAQMYSAIQTGGGVGMQTLDMSL
KALVGAGLVSLEHAREKARAPESI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=140681 TO66_RS29530 WP_044465568.1 6470504..6471538(-) (pilT) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
ATGGATATCACCGAGTTGCTGGCCTTCAGCGTCAAACAGGGCGCTTCGGACCTGCACCTCTCGGCGGGTTTGCCGCCGAT
GATCCGCATCGACGGCGATATTCGCCGGATCAACCTGCCGCTGCTGGAGGCGCCGCAGGTGCACGAGCTGATCCTCGCCA
GCATGACGGACCCGCAGCGGGCCGAGTTCAAGGCGGCGCTGGAGATCGATTATTCCTTCGCGCTGCCGGGCGTGGCGCGT
TTCCGGGTCAATGCCTTCAACCACCAGCGCGGCAATGGCGCGGTCTTTCGCAGCATTCCCGACCAGGTGCCGAGCCTGGA
CGCGCTGGGCCTGGGCGAGGTGTTTCGCCGGATCAGCGAAGCGCCCCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGGT
CGGGCAAGTCCACCACCCTGGCGGCGATGATCGACCACCTGAACCAGCACCGGCACCAGCACATCCTCACCATCGAGGAT
CCTATCGAGTTCATCCATGAGCCGAAAAAGTGCCTGATCCACCAGCGCGAGGTGCAGCGCGACACGCACAGTTTCTCCAG
CGCCCTGCGCTCGGCCTTGCGCGAAGACCCCGATGTGATCCTGGTCGGCGAGCTGCGCGACCTGGAAACCATTCGCCTGG
CGTTGACCGCGGCCGAGACCGGGCACCTGGTGTTCGCCACCTTGCACACCACGTCGGCGGCGAAAACCGTGGACCGGGTG
GTGGATGTGTTCCCCGCCGAGGAAAAGGCCATGGTCCGCTCCGTGCTGTCGGAGTCGTTGCAGGCGGTGATTTCCCAGGC
GTTGCTCAAGCGTGTCGGCGGTGGCCGGGTCGCGGCTCACGAAATCATGCTGGGGACCGCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACGGGCGGCGGAGTGGGGATGCAGACCCTGGACATGAGCCTG
AAGGCGCTGGTGGGGGCGGGGCTGGTCAGCCTGGAGCACGCCCGGGAGAAGGCGCGGGCGCCGGAGAGCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C5E906

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.616

100

0.776
  pilT Pseudomonas stutzeri DSM 10701

75.872

100

0.759
  pilT Acinetobacter baumannii D1279779

72.384

100

0.724
  pilT Acinetobacter baumannii strain A118

72.384

100

0.724
  pilT Acinetobacter nosocomialis M2

72.384

100

0.724
  pilT Acinetobacter baylyi ADP1

71.802

100

0.718
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Legionella pneumophila strain Lp02

66.272

98.256

0.651
  pilT Legionella pneumophila strain ERS1305867

66.272

98.256

0.651
  pilT Neisseria meningitidis 8013

64.058

100

0.642
  pilT Neisseria gonorrhoeae MS11

63.768

100

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.035

100

0.52
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Vibrio cholerae strain A1552

42.462

94.477

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387

Multiple sequence alignment