Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SB85_RS09705 Genome accession   NZ_CP010409
Coordinates   2341723..2342862 (+) Length   379 a.a.
NCBI ID   WP_017907532.1    Uniprot ID   A0A6N7QD09
Organism   Xanthomonas sacchari strain R1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2336723..2347862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SB85_RS09680 (SB85_09750) - 2336958..2338142 (-) 1185 WP_043093329.1 cystathionine gamma-synthase -
  SB85_RS09685 (SB85_09755) - 2338227..2339597 (-) 1371 WP_043093331.1 pyridoxal-phosphate dependent enzyme -
  SB85_RS09690 (SB85_09760) - 2339788..2340006 (-) 219 WP_010340228.1 YdcH family protein -
  SB85_RS09695 (SB85_09765) - 2340341..2341213 (-) 873 WP_043095258.1 OmpA family protein -
  SB85_RS09700 (SB85_09770) - 2341231..2341623 (-) 393 WP_026144112.1 DUF4398 domain-containing protein -
  SB85_RS09705 (SB85_09775) pilU 2341723..2342862 (+) 1140 WP_017907532.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SB85_RS09710 (SB85_09780) maiA 2343351..2344007 (-) 657 WP_043093332.1 maleylacetoacetate isomerase -
  SB85_RS09715 (SB85_09785) - 2344097..2345086 (-) 990 WP_017908490.1 fumarylacetoacetate hydrolase family protein -
  SB85_RS09720 (SB85_09790) - 2345432..2346805 (+) 1374 WP_043093333.1 DEAD/DEAH box helicase -
  SB85_RS09725 (SB85_09795) queD 2346984..2347343 (+) 360 WP_017916250.1 6-carboxytetrahydropterin synthase QueD -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42083.38 Da        Isoelectric Point: 6.2387

>NTDB_id=136944 SB85_RS09705 WP_017907532.1 2341723..2342862(+) (pilU) [Xanthomonas sacchari strain R1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGATGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELHLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDYRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLSLNLRAVISQRLVKDSSGRRRPASEVLLNTPMIRDLLRRGQVHEIKQAMEESLEEGMETFDQCL
FRMVKQGQIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYDSGPSAAPRITHGFV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=136944 SB85_RS09705 WP_017907532.1 2341723..2342862(+) (pilU) [Xanthomonas sacchari strain R1]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACAGGAGCACCGGTCTA
TATCAAGATCGAAGGCAAGCTGTACCCGCTCGGCGCCACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGGGAGCTGGAATTGAACATGGCCATCGCCCTGCAGGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCAAGATCCCCAGCATCGA
GGAGCTGCACCTGCCGCAGGTGCTCAAGGACGTGATCATGACCCCGCGCGGGCTGGTCCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACTACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCACTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCAGAAACCATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGACATCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCACACAAGAACGTGCTGATGAACCTGTCATTGAACCTGCGCGCGGTGATCTCGCAGCG
CCTGGTCAAGGACAGCAGCGGCCGCCGCCGGCCGGCCTCGGAAGTGCTGCTGAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTCCACGAGATCAAGCAGGCGATGGAGGAATCGCTGGAGGAAGGCATGGAGACCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGCAGGGCCAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCGGCAGCGGCGAGCACGATCCCTACGCCGACTACGACAGCGGCCCCAGCGCCGCGCCGCGGA
TCACCCACGGCTTCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N7QD09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

94.723

0.493
  pilU Vibrio cholerae strain A1552

49.722

94.987

0.472
  pilU Acinetobacter baylyi ADP1

50

93.404

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.18

99.736

0.441
  pilT Pseudomonas aeruginosa PAK

43.491

89.182

0.388
  pilT Acinetobacter nosocomialis M2

42.308

89.182

0.377
  pilT Acinetobacter baumannii D1279779

42.308

89.182

0.377
  pilT Acinetobacter baumannii strain A118

42.308

89.182

0.377
  pilT Acinetobacter baylyi ADP1

41.471

89.71

0.372
  pilT Neisseria meningitidis 8013

40.346

91.557

0.369
  pilT Neisseria gonorrhoeae MS11

40.346

91.557

0.369
  pilT Legionella pneumophila strain Lp02

41.42

89.182

0.369
  pilT Legionella pneumophila strain ERS1305867

41.42

89.182

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.916

88.127

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364

Multiple sequence alignment