Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FORC9_RS01930 Genome accession   NZ_CP009984
Coordinates   393326..394363 (+) Length   345 a.a.
NCBI ID   WP_060530716.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_009     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388326..399363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC9_RS01895 (FORC9_0329) hemW 388580..389752 (-) 1173 WP_060530712.1 radical SAM family heme chaperone HemW -
  FORC9_RS01900 (FORC9_0330) - 389762..390364 (-) 603 WP_011079456.1 XTP/dITP diphosphatase -
  FORC9_RS01905 (FORC9_0331) - 390375..390806 (-) 432 WP_011151200.1 DUF4426 domain-containing protein -
  FORC9_RS01910 (FORC9_0332) yggU 390849..391139 (-) 291 WP_060530713.1 DUF167 family protein YggU -
  FORC9_RS01915 (FORC9_0333) - 391139..391696 (-) 558 WP_013571095.1 YggT family protein -
  FORC9_RS01920 (FORC9_0334) proC 391738..392556 (-) 819 WP_060530714.1 pyrroline-5-carboxylate reductase -
  FORC9_RS01925 (FORC9_0335) - 392591..393301 (-) 711 WP_060530715.1 YggS family pyridoxal phosphate-dependent enzyme -
  FORC9_RS01930 (FORC9_0336) pilT 393326..394363 (+) 1038 WP_060530716.1 type IV pilus twitching motility protein PilT Machinery gene
  FORC9_RS01935 (FORC9_0337) pilU 394382..395488 (+) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FORC9_RS01940 (FORC9_0338) ruvX 395581..396003 (-) 423 WP_060530718.1 Holliday junction resolvase RuvX -
  FORC9_RS01945 (FORC9_0339) - 396025..396588 (-) 564 WP_011079465.1 YqgE/AlgH family protein -
  FORC9_RS01950 (FORC9_0340) gshB 396643..397596 (-) 954 WP_013571098.1 glutathione synthase -
  FORC9_RS01955 (FORC9_0341) rsmE 397611..398342 (-) 732 WP_060530721.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FORC9_RS01960 (FORC9_0342) - 398454..399149 (-) 696 WP_060530722.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38413.00 Da        Isoelectric Point: 6.3701

>NTDB_id=133454 FORC9_RS01930 WP_060530716.1 393326..394363(+) (pilT) [Vibrio vulnificus strain FORC_009]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGVPAFNHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTVPTTIPTLEDLDCPQIFEKIAKSEKGLVLVTGPTGSGKSTTLAAMVDYINRNYNKHILTIED
PIEFVHPNQKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLIAQGKVAKEEVDAKIEIETIQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=133454 FORC9_RS01930 WP_060530716.1 393326..394363(+) (pilT) [Vibrio vulnificus strain FORC_009]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAACGCATCAGATCTACATCTTTCTGCGGGTGTGCCTCCTAT
GGTACGCATAGATGGCGATGTAAGAAAGCTCGGTGTGCCCGCATTTAATCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAAAAGTTGGAAGTCGATTTTTCCTTTGAGTTGCCTAACGTTGGCCGT
TTTCGTGTTAACGCCTTTAACCAATCTCGCGGTTGCTCTGCGGTGTTTCGTACTGTTCCAACGACGATTCCGACATTGGA
AGATCTCGACTGCCCACAGATTTTTGAAAAAATTGCGAAGAGTGAAAAAGGCTTAGTGCTGGTCACCGGGCCAACAGGTT
CGGGTAAATCGACCACGTTAGCGGCGATGGTGGATTACATTAATCGCAATTACAACAAACACATTCTGACCATTGAAGAT
CCAATTGAATTTGTTCACCCAAATCAGAAATGTTTGATTAACCAACGCGAAGTTCATCGCGACACCCACAGTTTTAAAAA
TGCCCTGCGCAGCGCATTGCGTGAGGACCCAGACGTTATCTTGGTGGGTGAGTTACGTGACCAAGAGACCATCAGCCTAG
CGCTTACCGCGGCAGAAACGGGTCACTTGGTGTTTGGTACGTTGCACACCAGTAGTGCAGCAAAAACCATTGACCGTATT
ATCGATGTGTTCCCTGGTAGTGACAAAGACATGGTGCGCTCAATGCTTTCTGAATCATTGCGCGCCGTTATCGCACAGAA
ATTGCTGAAACGCATTGGAGGCGGGCGAGTGGCGTGTCATGAGATCATGATGGCAACGCCCGCCATTCGTAACTTGATCC
GTGAAGACAAAGTGGCACAAATGTACTCCATTATCCAAACGGGGGCTGCGCACGGCATGCAGACGATGGAACAGAATGCG
CGTCAGTTGATTGCACAGGGCAAAGTGGCCAAAGAAGAAGTCGACGCCAAAATTGAGATTGAAACTATCCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.406

100

0.884
  pilT Vibrio cholerae strain A1552

88.406

100

0.884
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Acinetobacter baylyi ADP1

71.865

94.783

0.681
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Pseudomonas aeruginosa PAK

67.941

98.551

0.67
  pilT Legionella pneumophila strain Lp02

68.278

95.942

0.655
  pilT Legionella pneumophila strain ERS1305867

68.278

95.942

0.655
  pilT Neisseria meningitidis 8013

65.766

96.522

0.635
  pilT Neisseria gonorrhoeae MS11

65.465

96.522

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

99.42

0.516
  pilU Pseudomonas stutzeri DSM 10701

41.916

96.812

0.406
  pilU Vibrio cholerae strain A1552

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374

Multiple sequence alignment