Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPICK_RS15550 Genome accession   NZ_CP009788
Coordinates   3417581..3418651 (-) Length   356 a.a.
NCBI ID   WP_039744753.1    Uniprot ID   A0A0B5BCY9
Organism   Geobacter pickeringii strain G13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3412581..3423651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPICK_RS15520 (GPICK_15520) mqnE 3412697..3413779 (-) 1083 WP_039744744.1 aminofutalosine synthase MqnE -
  GPICK_RS15525 (GPICK_15525) - 3413776..3414384 (-) 609 WP_039744746.1 UbiX family flavin prenyltransferase -
  GPICK_RS15530 (GPICK_15530) - 3414381..3415277 (-) 897 WP_039744748.1 4-hydroxybenzoate octaprenyltransferase -
  GPICK_RS15535 (GPICK_15535) - 3415267..3415797 (-) 531 WP_039744750.1 hypothetical protein -
  GPICK_RS15540 (GPICK_15540) - 3415794..3417227 (-) 1434 WP_144400109.1 UbiD family decarboxylase -
  GPICK_RS15550 (GPICK_15550) pilT 3417581..3418651 (-) 1071 WP_039744753.1 type IV pilus twitching motility protein PilT Machinery gene
  GPICK_RS15555 (GPICK_15555) - 3418665..3420314 (-) 1650 WP_039744754.1 ATPase, T2SS/T4P/T4SS family -
  GPICK_RS15560 (GPICK_15560) - 3420503..3421507 (+) 1005 WP_039744756.1 hypothetical protein -
  GPICK_RS15565 (GPICK_15565) - 3421571..3422527 (-) 957 WP_039744758.1 transglycosylase domain-containing protein -
  GPICK_RS15570 (GPICK_15570) pdxA 3422524..3423576 (-) 1053 WP_052263462.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39704.93 Da        Isoelectric Point: 7.0674

>NTDB_id=132226 GPICK_RS15550 WP_039744753.1 3417581..3418651(-) (pilT) [Geobacter pickeringii strain G13]
MARIDALFKMLKEQGASDLHLSSGAPPIFRLHGEMARQNFKPLTHEELTSILYEILDEKQKETFETRHDLDFAYAIPGLA
RFRGNYLMTHRGVAAVFRIIPSKILSADDLGLPDGVRKMTQFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLE
DPLEFIHENKMSLLNQRQIGEHSDSFTNALRAALREDPDVILVGEMRDLETISLAMSAAETGHLVFGTLHTNSAAKTIDR
IIDVFPKDAQEQTRTMLSESLKGVVCQQLLRTADGKGRCAALEIMLGTPAIGNLIREGKTFQIPSIIQTAKKDGMQLMDQ
HLLDLLKMKKIAPEEAYRCAQDKKQFEQYLPEKPAQ

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=132226 GPICK_RS15550 WP_039744753.1 3417581..3418651(-) (pilT) [Geobacter pickeringii strain G13]
ATGGCACGCATAGACGCACTCTTCAAAATGCTCAAGGAGCAGGGAGCATCGGACCTGCACCTCTCCTCCGGGGCCCCCCC
GATCTTCCGACTCCACGGGGAGATGGCCCGCCAGAACTTCAAGCCCCTCACCCACGAGGAGCTGACGTCGATCCTCTACG
AGATCCTGGACGAGAAGCAGAAGGAGACCTTCGAGACGAGGCACGACCTGGACTTCGCCTACGCCATCCCGGGGCTTGCC
CGTTTCCGGGGCAACTACCTGATGACCCACCGGGGGGTCGCCGCGGTCTTCCGGATCATCCCGAGCAAGATCCTGTCGGC
CGACGATCTGGGGCTTCCGGATGGGGTCCGGAAGATGACCCAGTTCAAGAAGGGGCTGGTGCTGGTCACCGGGCCCACGG
GGTCGGGGAAGTCGACGACCCTGGCCGCGATGATCGATCTCATCAATGCCACCCGCAAGGAGCATATCCTCACGCTGGAG
GACCCCCTGGAGTTCATCCACGAGAACAAGATGTCGCTCCTCAACCAGCGTCAGATCGGGGAGCACTCCGACAGTTTCAC
CAACGCCCTGCGGGCGGCATTGCGGGAAGACCCCGACGTGATCCTCGTCGGTGAGATGCGGGATCTGGAAACCATCAGCC
TCGCCATGAGCGCCGCCGAAACCGGACACCTCGTTTTCGGGACCCTGCACACCAACTCGGCGGCCAAGACCATTGACCGG
ATCATCGACGTCTTCCCCAAAGACGCCCAGGAGCAGACCCGCACCATGCTCTCCGAGTCCCTCAAGGGGGTCGTCTGTCA
GCAGCTTCTCCGGACTGCCGACGGCAAGGGGCGTTGTGCGGCGCTGGAGATCATGCTCGGTACGCCGGCCATCGGCAACC
TGATCCGGGAGGGTAAGACCTTCCAGATACCTTCCATCATCCAGACCGCCAAGAAGGACGGGATGCAGTTGATGGACCAG
CACCTGCTCGACCTCCTCAAGATGAAGAAGATCGCGCCGGAGGAGGCGTACCGCTGTGCCCAGGACAAGAAGCAGTTCGA
ACAGTATCTGCCGGAAAAACCGGCGCAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B5BCY9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

57.1

92.978

0.531
  pilT Legionella pneumophila strain Lp02

57.1

92.978

0.531
  pilT Pseudomonas stutzeri DSM 10701

54.81

96.348

0.528
  pilT Acinetobacter baylyi ADP1

54.519

96.348

0.525
  pilT Pseudomonas aeruginosa PAK

53.644

96.348

0.517
  pilT Acinetobacter nosocomialis M2

53.353

96.348

0.514
  pilT Acinetobacter baumannii D1279779

53.353

96.348

0.514
  pilT Acinetobacter baumannii strain A118

53.353

96.348

0.514
  pilT Neisseria meningitidis 8013

55.623

92.416

0.514
  pilT Neisseria gonorrhoeae MS11

55.623

92.416

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.312

96.348

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.711

93.258

0.492
  pilT Vibrio cholerae strain A1552

52.711

93.258

0.492
  pilU Vibrio cholerae strain A1552

39.349

94.944

0.374
  pilU Pseudomonas stutzeri DSM 10701

40.06

93.258

0.374
  pilU Acinetobacter baylyi ADP1

38.507

94.101

0.362

Multiple sequence alignment