Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPICK_RS08600 Genome accession   NZ_CP009788
Coordinates   1908301..1909398 (-) Length   365 a.a.
NCBI ID   WP_039742249.1    Uniprot ID   -
Organism   Geobacter pickeringii strain G13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1903301..1914398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPICK_RS18025 (GPICK_08580) - 1903560..1903772 (-) 213 WP_039742239.1 type IV pilin protein -
  GPICK_RS08585 (GPICK_08585) - 1903995..1905374 (-) 1380 WP_039742241.1 sigma-54 dependent transcriptional regulator -
  GPICK_RS08590 (GPICK_08590) - 1905410..1907008 (-) 1599 WP_330217043.1 ATP-binding protein -
  GPICK_RS08595 (GPICK_08595) - 1907053..1908270 (-) 1218 WP_039742246.1 type II secretion system F family protein -
  GPICK_RS08600 (GPICK_08600) pilT 1908301..1909398 (-) 1098 WP_039742249.1 type IV pilus twitching motility protein PilT Machinery gene
  GPICK_RS08605 (GPICK_08605) pilB 1909413..1911119 (-) 1707 WP_039742252.1 type IV-A pilus assembly ATPase PilB -
  GPICK_RS08610 (GPICK_08610) aroE 1911183..1912043 (-) 861 WP_039742254.1 shikimate dehydrogenase -
  GPICK_RS08615 (GPICK_08615) - 1912046..1913908 (-) 1863 WP_039742256.1 DUF2723 domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40693.20 Da        Isoelectric Point: 8.1649

>NTDB_id=132221 GPICK_RS08600 WP_039742249.1 1908301..1909398(-) (pilT) [Geobacter pickeringii strain G13]
MANMHQLLTELVNRGGSDLHLTTNTPPQIRVDGKLIPLDMPPLNAIDTKQLCYSILTEQQKHKFEEVNELDLSFGIKGLS
RFRGNVFVQRGAVAGVFRVIPYKILTFEELGLPPVVKDLAEKPRGLILVTGPTGSGKSTTLAAIIDKINNERHEHIVTVE
DPIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSCVQTINR
IVDVFPPYQQPQVRAQLSFVLEGVISQSLLPKASGKGRVLSLEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMQTMN
QSLYSLLQKRLVAMDVAMARSSDPDELKQMLTSGMRPQGPRPTMR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=132221 GPICK_RS08600 WP_039742249.1 1908301..1909398(-) (pilT) [Geobacter pickeringii strain G13]
ATGGCAAACATGCACCAATTGCTGACCGAACTGGTGAATCGGGGTGGGTCCGACCTCCACCTTACCACTAACACCCCTCC
TCAAATTCGGGTTGATGGTAAGCTTATTCCCCTGGACATGCCCCCCCTGAACGCGATCGATACCAAGCAGCTCTGTTACA
GTATCCTTACCGAACAGCAGAAGCACAAGTTCGAGGAAGTCAATGAGCTTGATCTCTCCTTCGGCATCAAGGGGCTTTCC
CGTTTCAGGGGGAACGTCTTCGTCCAGCGTGGCGCGGTTGCCGGGGTTTTCCGGGTCATCCCCTACAAGATCCTCACCTT
TGAAGAGCTTGGCCTTCCGCCCGTCGTCAAGGATCTGGCGGAAAAGCCCCGGGGACTCATTCTGGTGACCGGTCCCACCG
GCAGTGGCAAATCGACGACGCTCGCCGCCATTATCGACAAGATCAACAATGAGCGCCACGAGCATATCGTGACGGTGGAA
GATCCGATCGAATACCTCCATCCCCACAAGAGTTGTGTCGTGAACCAGCGGGAAGTCGGTGCGGACACTAAGAGCTTCAA
GAACGCCCTCAAGTACATCCTCCGTCAGGACCCGGACGTGGTACTCGTCGGAGAACTCAGGGACCTGGAAACCATCGAAG
CGGCGCTGACTCTGGCGGAGACTGGGCACCTCTGTCTCGCCACGCTCCACACCAACTCCTGTGTGCAGACCATCAACCGT
ATCGTCGACGTCTTTCCCCCGTATCAGCAGCCCCAGGTCCGTGCCCAGCTTTCGTTCGTCCTGGAAGGAGTCATTTCCCA
GTCACTGCTCCCCAAGGCCAGCGGCAAGGGGAGGGTGCTGTCGCTCGAGGTCATGGTCCCCAACCCTGCCATCCGCAACC
TGATCCGCGAGGACAAGATCCACCAGATCTACTCGCAGATGCAGGTTGGACAGGAGAAGTTCGGGATGCAGACCATGAAC
CAGTCGCTGTACAGTCTGCTTCAGAAACGCCTCGTTGCCATGGATGTGGCGATGGCACGCTCATCGGATCCGGATGAACT
TAAACAGATGCTCACAAGCGGGATGAGGCCCCAGGGACCGCGTCCAACCATGAGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

55.224

91.781

0.507
  pilT Legionella pneumophila strain ERS1305867

55.224

91.781

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.437

95.342

0.49
  pilT Acinetobacter baylyi ADP1

51.014

94.521

0.482
  pilT Pseudomonas aeruginosa PAK

50.725

94.521

0.479
  pilT Pseudomonas stutzeri DSM 10701

50.435

94.521

0.477
  pilT Acinetobacter nosocomialis M2

50.145

94.521

0.474
  pilT Acinetobacter baumannii D1279779

50.145

94.521

0.474
  pilT Acinetobacter baumannii strain A118

50.145

94.521

0.474
  pilT Neisseria meningitidis 8013

49.401

91.507

0.452
  pilT Neisseria gonorrhoeae MS11

48.649

91.233

0.444
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

90.411

0.444
  pilT Vibrio cholerae strain A1552

49.091

90.411

0.444
  pilU Vibrio cholerae strain A1552

42.286

95.89

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.111

98.63

0.405
  pilU Acinetobacter baylyi ADP1

38.617

95.068

0.367

Multiple sequence alignment