Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NC16_RS18075 Genome accession   NZ_CP009769
Coordinates   3971015..3972031 (-) Length   338 a.a.
NCBI ID   WP_075278317.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain BC1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3966015..3977031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NC16_RS18060 (NC16_17380) nsrR 3966380..3966805 (-) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -
  NC16_RS18065 (NC16_17385) - 3967116..3968687 (+) 1572 WP_075278315.1 methyl-accepting chemotaxis protein -
  NC16_RS18070 (NC16_17390) - 3969214..3971046 (+) 1833 WP_075278316.1 sensor domain-containing diguanylate cyclase -
  NC16_RS18075 (NC16_17395) pilT 3971015..3972031 (-) 1017 WP_075278317.1 type IV pilus twitching motility protein PilT Machinery gene
  NC16_RS18080 (NC16_17400) - 3972052..3972765 (+) 714 WP_075278318.1 YggS family pyridoxal phosphate-dependent enzyme -
  NC16_RS18085 (NC16_17405) proC 3972860..3973681 (+) 822 WP_043902046.1 pyrroline-5-carboxylate reductase -
  NC16_RS18090 (NC16_17410) - 3973730..3974284 (+) 555 WP_010279107.1 YggT family protein -
  NC16_RS18095 (NC16_17415) yggU 3974281..3974571 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  NC16_RS18100 (NC16_17420) rdgB 3974589..3975182 (+) 594 WP_075278319.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  NC16_RS18105 (NC16_17425) hemW 3975175..3976317 (+) 1143 WP_075278320.1 radical SAM family heme chaperone HemW -
  NC16_RS18110 - 3976546..3976806 (+) 261 WP_015841595.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36711.91 Da        Isoelectric Point: 6.6533

>NTDB_id=132062 NC16_RS18075 WP_075278317.1 3971015..3972031(-) (pilT) [Pectobacterium brasiliense strain BC1]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLAALQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLFPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=132062 NC16_RS18075 WP_075278317.1 3971015..3972031(-) (pilT) [Pectobacterium brasiliense strain BC1]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCGGCTCAGCGCGAGCAATTGCGGCAGGCTGGGCAGGTGGACGGGGCATTAATGCTGCCCGATGGACAA
CGCCTGCGGGTCAATGTATTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTATTATCCCGTCCGTTCAACCTTCATT
GGCGGCGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTCATCCATGCCAGCCGGCGTTGCCTGATTCAACAGCGGGAGATTGGTGCGCACAGTGCGTCCTTTGC
GCAGGCGCTACGTGCTGCATTGCGGGAGGATCCCGATGTTATTCTGCTGGGCGAGTTGCGCGATACGGAGACGATTCGAC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTATCCACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGTTTCCCGCTGCGCAAGGTGGTCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Neisseria meningitidis 8013

50

98.225

0.491
  pilT Neisseria gonorrhoeae MS11

49.699

98.225

0.488
  pilT Pseudomonas stutzeri DSM 10701

48.943

97.929

0.479
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.64

97.929

0.476
  pilT Legionella pneumophila strain Lp02

48.64

97.929

0.476
  pilT Legionella pneumophila strain ERS1305867

48.64

97.929

0.476
  pilT Acinetobacter nosocomialis M2

48.338

97.929

0.473
  pilT Acinetobacter baumannii D1279779

48.338

97.929

0.473
  pilT Acinetobacter baumannii strain A118

48.338

97.929

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367

Multiple sequence alignment