Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LH22_RS06010 Genome accession   NZ_CP009454
Coordinates   1291805..1292797 (+) Length   330 a.a.
NCBI ID   WP_038644906.1    Uniprot ID   -
Organism   Pantoea rwandensis strain ND04     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1286805..1297797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LH22_RS05980 (LH22_06110) - 1287127..1287453 (+) 327 WP_034823275.1 YggL family protein -
  LH22_RS05985 (LH22_06120) - 1287711..1288427 (+) 717 WP_038644898.1 DUF2884 domain-containing protein -
  LH22_RS05990 (LH22_06125) hemW 1288777..1289907 (-) 1131 WP_038644900.1 radical SAM family heme chaperone HemW -
  LH22_RS05995 (LH22_06130) - 1289900..1290493 (-) 594 WP_038644902.1 XTP/dITP diphosphatase -
  LH22_RS06000 (LH22_06135) - 1290514..1291068 (-) 555 WP_034823271.1 YggT family protein -
  LH22_RS06005 (LH22_06140) - 1291081..1291788 (-) 708 WP_038644904.1 YggS family pyridoxal phosphate-dependent enzyme -
  LH22_RS06010 (LH22_06145) pilT 1291805..1292797 (+) 993 WP_038644906.1 type IV pilus twitching motility protein PilT Machinery gene
  LH22_RS06015 (LH22_06150) ruvX 1292869..1293285 (-) 417 WP_038644908.1 Holliday junction resolvase RuvX -
  LH22_RS06020 (LH22_06155) - 1293285..1293848 (-) 564 WP_038644910.1 YqgE/AlgH family protein -
  LH22_RS06025 (LH22_06160) gshB 1293931..1294875 (-) 945 WP_038644912.1 glutathione synthase -
  LH22_RS06030 (LH22_06165) rsmE 1294966..1295697 (-) 732 WP_038644914.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LH22_RS06035 (LH22_06170) endA 1295772..1296479 (-) 708 WP_038644916.1 deoxyribonuclease I -
  LH22_RS06040 (LH22_06175) - 1296556..1297068 (-) 513 WP_045831736.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35980.06 Da        Isoelectric Point: 6.2646

>NTDB_id=129382 LH22_RS06010 WP_038644906.1 1291805..1292797(+) (pilT) [Pantoea rwandensis strain ND04]
MDLDEVVALSVKHNAADLHLCSGHLPHWRRQGVLEPIPHQTALDGDWLETFLQQWLSGVQQDELEASGHVDFAITLESGV
RLRANLFMQRHGLSLALRLIASQAPNINQLQLPDVAQQLLQQEEGLILITGATGSGKSTTLAAMVDSLNREQARHIITLE
DPIEFVHHSQRSLIQQREVGTHTASFQQGLKAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGATQAVDR
LVDVFPAEEKNLVRTQLAGSLKAVLAQRLVPAKNGGRIGLFEVLVATPAVVNLIREGKMHQLPGVLQTGAQAGMQTFAQS
EQARQQAGLI

Nucleotide


Download         Length: 993 bp        

>NTDB_id=129382 LH22_RS06010 WP_038644906.1 1291805..1292797(+) (pilT) [Pantoea rwandensis strain ND04]
ATGGATTTGGATGAAGTTGTGGCCCTTAGTGTAAAGCATAACGCCGCCGATCTGCACCTTTGCAGCGGACATTTACCGCA
CTGGCGACGGCAGGGCGTGCTGGAGCCGATCCCTCATCAAACCGCATTGGATGGTGACTGGCTGGAAACCTTCTTGCAAC
AGTGGCTCAGCGGCGTGCAGCAGGATGAGCTGGAAGCGAGCGGGCACGTTGATTTTGCCATCACCCTGGAAAGCGGCGTG
CGTTTGCGTGCCAACCTGTTTATGCAGCGCCACGGTTTATCGCTGGCGCTGCGCTTGATTGCCAGCCAGGCACCCAACAT
CAACCAGTTGCAACTGCCCGATGTGGCACAGCAATTGTTGCAGCAGGAAGAGGGGCTGATCTTAATCACGGGTGCCACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGGTGGACAGCCTCAATCGCGAACAGGCACGCCACATTATTACGCTGGAA
GATCCGATTGAGTTTGTGCATCACAGCCAGCGCTCGTTGATACAGCAGCGGGAAGTGGGTACACACACGGCGTCATTCCA
GCAAGGATTGAAAGCGGCGCTGCGCGAAGACCCGGATGTGATTCTGCTGGGTGAGCTACGGGACAGCGAGACCATTCGGC
TGGCGCTGACGGCGGCAGAGACCGGGCATCTGGTACTGGCAACGCTGCACACGCGCGGTGCCACGCAAGCGGTGGATCGG
CTGGTGGATGTGTTTCCAGCCGAGGAAAAAAACCTGGTGCGCACCCAGCTGGCGGGCAGTTTGAAAGCGGTGCTGGCGCA
GCGTTTGGTGCCGGCAAAAAATGGCGGTCGGATTGGTTTGTTCGAGGTGCTGGTGGCCACGCCTGCGGTGGTCAATTTAA
TCCGCGAGGGCAAGATGCATCAATTGCCTGGCGTGCTGCAAACCGGCGCGCAGGCAGGGATGCAGACGTTCGCGCAGAGC
GAACAGGCACGTCAACAGGCGGGATTGATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

48.503

100

0.491
  pilT Neisseria meningitidis 8013

48.503

100

0.491
  pilT Vibrio cholerae strain A1552

48.457

98.182

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.457

98.182

0.476
  pilT Pseudomonas stutzeri DSM 10701

46.061

100

0.461
  pilT Acinetobacter baylyi ADP1

46.061

100

0.461
  pilT Acinetobacter nosocomialis M2

45.455

100

0.455
  pilT Acinetobacter baumannii D1279779

45.455

100

0.455
  pilT Acinetobacter baumannii strain A118

45.455

100

0.455
  pilT Pseudomonas aeruginosa PAK

45.152

100

0.452
  pilT Legionella pneumophila strain ERS1305867

45.679

98.182

0.448
  pilT Legionella pneumophila strain Lp02

45.679

98.182

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.025

96.364

0.424
  pilU Pseudomonas stutzeri DSM 10701

41.121

97.273

0.4
  pilU Vibrio cholerae strain A1552

40.498

97.273

0.394
  pilU Acinetobacter baylyi ADP1

38.509

97.576

0.376

Multiple sequence alignment