Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG71_RS08425 Genome accession   NZ_CP009450
Coordinates   1791030..1792010 (+) Length   326 a.a.
NCBI ID   WP_043082077.1    Uniprot ID   -
Organism   Pluralibacter gergoviae strain FB2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1786030..1797010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG71_RS08390 (LG71_08560) ansB 1786076..1787122 (+) 1047 WP_043082070.1 L-asparaginase 2 -
  LG71_RS08395 (LG71_08565) - 1787305..1787652 (+) 348 WP_043082071.1 hypothetical protein -
  LG71_RS08400 (LG71_08570) hemW 1787701..1788840 (-) 1140 WP_043082072.1 radical SAM family heme chaperone HemW -
  LG71_RS08405 (LG71_08575) - 1788840..1789427 (-) 588 WP_043082073.1 XTP/dITP diphosphatase -
  LG71_RS08410 (LG71_08580) yggU 1789415..1789726 (-) 312 WP_043082074.1 DUF167 family protein YggU -
  LG71_RS08415 (LG71_08585) - 1789723..1790289 (-) 567 WP_043082075.1 YggT family protein -
  LG71_RS08420 (LG71_08590) - 1790311..1791015 (-) 705 WP_043082076.1 YggS family pyridoxal phosphate-dependent enzyme -
  LG71_RS08425 (LG71_08595) pilT 1791030..1792010 (+) 981 WP_043082077.1 type IV pilus twitching motility protein PilT Machinery gene
  LG71_RS08430 (LG71_08600) - 1792434..1793087 (+) 654 WP_043082078.1 LuxR C-terminal-related transcriptional regulator -
  LG71_RS08435 (LG71_08605) ruvX 1793124..1793540 (-) 417 WP_043082079.1 Holliday junction resolvase RuvX -
  LG71_RS08440 (LG71_08610) - 1793540..1794103 (-) 564 WP_043082080.1 YqgE/AlgH family protein -
  LG71_RS08445 (LG71_08615) gshB 1794202..1795149 (-) 948 WP_043082081.1 glutathione synthase -
  LG71_RS08450 (LG71_08620) rsmE 1795172..1795903 (-) 732 WP_043082082.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LG71_RS08455 (LG71_08625) endA 1795924..1796661 (-) 738 WP_043082083.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35365.64 Da        Isoelectric Point: 7.2385

>NTDB_id=129329 LG71_RS08425 WP_043082077.1 1791030..1792010(+) (pilT) [Pluralibacter gergoviae strain FB2]
MDIEEIVALSVKHNVSDLHLCSAGQVRWRRRGSLERAPFPAPDLQPLLARWLGEAGVQEWLLQRQCDFAVETAGGRRLRA
SAFQHIRGCSLVLRLLPDACPQLSELGAPQALGELLHEESGLILVTGATGSGKSTTLAAMVEHLNRQRDGHILTLEDPVE
FIHRSARCLIQQREVGAHCASFADALRVALRQDPDVILIGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLLDA
FPAGEKEQVRGQLAGSLCAVLAQKLLPDKLGGRVALYELLVNTPAAANLIREGKSHQLPGIMQTGQQAGMLTFSQSFQQR
QAAGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=129329 LG71_RS08425 WP_043082077.1 1791030..1792010(+) (pilT) [Pluralibacter gergoviae strain FB2]
ATGGATATAGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCGGGCAGGTGCG
ATGGCGGCGGCGGGGGAGCCTCGAGCGGGCGCCATTCCCGGCGCCGGATCTGCAGCCCCTGCTGGCCCGCTGGCTCGGAG
AGGCGGGGGTACAGGAGTGGCTGCTGCAGCGGCAGTGCGACTTTGCCGTCGAAACGGCGGGCGGCAGGCGGCTGCGCGCC
AGCGCTTTCCAGCATATTCGCGGCTGCTCGCTGGTGCTGCGCCTGCTGCCCGACGCGTGCCCACAGCTCTCTGAACTGGG
GGCGCCGCAGGCCCTTGGCGAGCTGCTCCACGAGGAGAGCGGGCTTATCCTCGTCACCGGAGCGACCGGCAGCGGCAAGT
CCACCACGCTTGCCGCGATGGTGGAACACCTCAACCGGCAGCGGGACGGGCATATTCTGACCCTGGAAGACCCGGTAGAG
TTTATTCACCGCAGCGCCCGCTGCCTGATCCAGCAGCGGGAGGTCGGCGCCCACTGCGCCTCGTTTGCGGACGCGCTGCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGATTGGCGAACTGCGCGACAGCGAGACTATTCGCCTGGCGCTGACCG
CTGCGGAGACCGGGCACCTGGTTCTGGCGACGCTGCACACCCGGGGCGCGGCCCAGGCCGTTGAGCGGCTGCTGGACGCC
TTCCCCGCCGGCGAAAAAGAGCAGGTGCGCGGCCAGCTGGCGGGCAGCCTGTGCGCGGTGCTGGCCCAGAAGCTGCTGCC
AGATAAGCTGGGCGGGCGGGTGGCGCTGTACGAACTGCTGGTAAATACGCCCGCCGCCGCCAACCTGATCCGCGAGGGCA
AGAGCCATCAACTGCCGGGCATCATGCAGACCGGGCAGCAGGCGGGCATGCTGACCTTCAGCCAGAGTTTTCAGCAGCGG
CAGGCCGCCGGGCGGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.826

98.773

0.472
  pilT Neisseria gonorrhoeae MS11

47.516

98.773

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Acinetobacter nosocomialis M2

46.729

98.466

0.46
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

42.236

98.773

0.417
  pilU Pseudomonas stutzeri DSM 10701

40.06

100

0.408
  pilU Acinetobacter baylyi ADP1

38.629

98.466

0.38
  pilB Vibrio cholerae strain A1552

31.234

100

0.365

Multiple sequence alignment