Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JV59_RS01030 Genome accession   NZ_CP009264
Coordinates   188713..189819 (+) Length   368 a.a.
NCBI ID   WP_038509526.1    Uniprot ID   -
Organism   Vibrio coralliilyticus strain OCN014     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 183713..194819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JV59_RS00995 (JV59_26475) - 184120..184629 (-) 510 WP_235284656.1 hypothetical protein -
  JV59_RS01000 (JV59_26480) - 184645..184902 (-) 258 WP_235284657.1 hypothetical protein -
  JV59_RS01005 (JV59_26485) yggU 185191..185481 (-) 291 WP_038509522.1 DUF167 family protein YggU -
  JV59_RS01010 (JV59_00990) - 185481..186038 (-) 558 WP_006958264.1 YggT family protein -
  JV59_RS01015 (JV59_00995) proC 186077..186895 (-) 819 WP_019277259.1 pyrroline-5-carboxylate reductase -
  JV59_RS01020 (JV59_01000) - 186923..187639 (-) 717 WP_038509524.1 YggS family pyridoxal phosphate-dependent enzyme -
  JV59_RS01025 (JV59_01005) pilT 187664..188701 (+) 1038 WP_006958261.1 type IV pilus twitching motility protein PilT Machinery gene
  JV59_RS01030 (JV59_01010) pilU 188713..189819 (+) 1107 WP_038509526.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JV59_RS01035 (JV59_26490) ruvX 189918..190340 (-) 423 WP_038509528.1 Holliday junction resolvase RuvX -
  JV59_RS01040 (JV59_01020) - 190358..190921 (-) 564 WP_019277262.1 YqgE/AlgH family protein -
  JV59_RS01045 (JV59_01025) gshB 190978..191928 (-) 951 WP_019277263.1 glutathione synthase -
  JV59_RS01050 (JV59_01030) rsmE 191942..192673 (-) 732 WP_038509531.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JV59_RS01055 (JV59_01035) - 192782..193486 (-) 705 WP_038509533.1 endonuclease -
  JV59_RS01060 (JV59_01040) - 193566..194063 (-) 498 WP_038514553.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41244.87 Da        Isoelectric Point: 5.1963

>NTDB_id=127844 JV59_RS01030 WP_038509526.1 188713..189819(+) (pilU) [Vibrio coralliilyticus strain OCN014]
MDINAYLQGMLTEKASDLYITVGAPILYRVDGELRPRGESLSEAEVLTLLHSTMDEERQSDYSHSREANFAVVRDFGRFR
VSAFYQRELPGAVIRRIETTIPTFDDLKLPETLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTERSGHILTVEDPI
EFVHEHQKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVIGQQLIRDKDGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMAKSQEIGMQTFDQALY
QLVVNDKITEEDALHSADSANDLRIMLKTQRGESFGGGALADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=127844 JV59_RS01030 WP_038509526.1 188713..189819(+) (pilU) [Vibrio coralliilyticus strain OCN014]
ATGGATATCAATGCTTATCTGCAAGGCATGCTGACTGAGAAAGCGTCAGACTTATACATCACTGTTGGTGCGCCGATACT
TTATCGTGTTGATGGAGAGCTGAGACCGCGTGGAGAGAGCTTATCGGAAGCCGAAGTGCTGACGCTACTCCACAGCACCA
TGGATGAAGAGCGCCAGAGTGACTATAGCCACAGTCGAGAAGCGAACTTTGCCGTAGTGCGGGATTTTGGTCGTTTTCGT
GTGTCCGCTTTCTACCAGCGAGAATTACCCGGTGCGGTGATTCGTCGAATCGAAACCACCATACCCACATTTGATGACTT
AAAACTGCCCGAAACTCTAAAAGATCTTTCCATTGCCAAGCGCGGTTTGGTACTAGTAGTGGGCGCCACAGGCTCCGGTA
AGTCGACCACTATGGCGGCGATGACCGGCTATCGAAATACTGAGCGTAGCGGCCATATTCTGACAGTTGAAGACCCGATT
GAGTTTGTCCATGAGCATCAGAAATGCATCGTCACGCAACGTGAGGTTGGCTTAGACACAGAAAGTTATGAAGTCGCGTT
GAAGAACTCTCTGCGTCAGGCTCCTGATATGATCTTGATCGGTGAGATCCGTTCGCGAGAGACCATGGAATACGCGATGA
CATTCGCCGAAACGGGGCATCTGTGTATGGCGACGTTGCACGCCAATAATGCAAACCAAGCTTTGGAAAGGATTCTGCAC
CTTGTACCGAAAGAGCAAAAAGAGCAGTTCTTGTTTGATCTCTCCATGAACCTTAAAGGTGTTATCGGTCAGCAGTTGAT
TCGAGATAAAGACGGCCAAGGTCGTCATGGCGTTTTTGAAATCTTACTCAACAGTCCACGAGTATCGGATCTGATTCGTC
GCGGGGACTTGCATGAACTCAAGTCAACCATGGCGAAATCTCAGGAAATTGGTATGCAGACGTTTGATCAGGCGCTTTAT
CAATTGGTAGTGAATGACAAAATTACTGAAGAAGATGCGCTGCATAGTGCGGATTCAGCCAATGACCTGCGTATTATGCT
CAAGACTCAACGAGGTGAAAGCTTCGGTGGTGGTGCGCTGGCAGATGTGAAAATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.543

92.12

0.41
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria gonorrhoeae MS11

39.71

93.75

0.372
  pilT Neisseria meningitidis 8013

39.71

93.75

0.372
  pilT Vibrio cholerae strain A1552

41.846

88.315

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.846

88.315

0.37

Multiple sequence alignment